Comparison of Static and Countermovement Jump Variables in Relation to Estimated Training Load and Subjective Measures of Fatigue

The purpose of this study was to compare changes in static and countermovement jump variables across a competitive season of collegiate soccer to estimated training load and subjective measures of fatigue. Monitoring data from 21 male collegiate soccer players were retrospectively examined. Nine vertical jump sessions occurred across the season in addition to daily training load assessment and daily mood-state assessment. Group average changes from the first testing session were calculated and compared to the group average training load for the 7 days preceding each vertical jump testing session for static and countermovement jump height and allometrically scaled peak power. Statistical analysis demonstrated strong relationships between changes in vertical jump height for both conditions, allometrically scaled peak power for static jumps, and estimated training load. The results indicate changes in static jump height and allometrically scaled peak power may be more useful athlete fatigue monitoring tools than countermovement jump variables

Establishing a Duration Standard for the Calculation of Session Rating of Perceived Exertion in Ncaa Division I Men’s Soccer

Objectives: The purpose of this study was to determine the best predictor of training and/or match load using session RPE. Design and Methods: 20 NCAA DI male soccer players participated in the study during the 2014 and 2015 competitive seasons. Players completed 15.20 ± 1.05 matches for a total of 304 individual data points and 29.90 ± 1.89. training sessions for a total of 598 individual data points. GPS variables (total distance, High-intensity running distance, and Player load) were analyzed with session RPE using Pearson product-moment correlations. To evaluate various methods of session RPE, “match duration” was recorded using eight different definitions: total match duration including warm-up and half-time, total match duration and warm-up, total match duration and half-time, total match duration only, minutes played including warm-up and half-time, minutes played and warm-up, minutes played and half-time, and minutes played only. A one-way ANOVA with repeated measures was used to determine if differences existed between the eight session RPE calculations. Results: Results from the ANOVA showed that all session RPE measures were significantly different from one another (P \u3c 0.05). Very large correlations were reported between session RPE calculated using minutes played and total distance (0.81), while session RPE calculated using match duration showed less magnitude (0.57). Conclusions: Minutes played should be used to calculate session RPE as it was found to most closely reflect the actual workloads incurred during competitive matches

Decoding brain basis of laughter and crying in natural scenes

Laughter and crying are universal signals of prosociality and distress, respectively. Here we investigated the functional brain basis of perceiving laughter and crying using naturalistic functional magnetic resonance imaging (fMRI) approach. We measured haemodynamic brain activity evoked by laughter and crying in three experiments with 100 subjects in each. The subjects i) viewed a 20-minute medley of short video clips, and ii) 30 min of a full-length feature film, and iii) listened to 13.5 min of a radio play that all contained bursts of laughter and crying. Intensity of laughing and crying in the videos and radio play was annotated by independent observes, and the resulting time series were used to predict hemodynamic activity to laughter and crying episodes. Multivariate pattern analysis (MVPA) was used to test for regional selectivity in laughter and crying evoked activations. Laughter induced widespread activity in ventral visual cortex and superior and middle temporal and motor cortices. Crying activated thalamus, cingulate cortex along the anterior-posterior axis, insula and orbitofrontal cortex. Both laughter and crying could be decoded accurately (66–77% depending on the experiment) from the BOLD signal, and the voxels contributing most significantly to classification were in superior temporal cortex. These results suggest that perceiving laughter and crying engage distinct neural networks, whose activity suppresses each other to manage appropriate behavioral responses to others’ bonding and distress signals

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

An Examination of the Workloads and the Effectiveness of an Athlete Monitoring Program in NCAA Division I Men's Soccer

The purposes of this dissertation were to examine common athlete training and monitoring practices in men’s collegiate soccer and to report the programming strategies and monitoring outcomes for an NCAA Division I men’s collegiate soccer team whose coaching and sport science staff collaborated on a daily basis. The following are the major findings of the dissertation. Study 1 – Coaches from all divisions of play responded to a custom survey. A majority of coaches developed an in-season training plan that varied both daily and weekly volume and intensity. One-third of the coaches performed no athlete monitoring, and a number of coaches performed purely subjective monitoring of training load and fatigue. Common athlete monitoring tools included sport performance, self-report questionnaires, and physical performance tests. Most coaches believed their athletes did not change or improved in all aspects of performance, while injuries were a mix of new and recurrent. Study 2 – Statistical differences in training load were found between each phase of the season, and training load variation was found with respect to the number of days before a match. Phasic training loads were highest during the pre-season and non-conference portions of the season and decreased significantly during conference play and the post-season. The daily training load values reflected the player groups’ match involvement and therefore led to different loading strategies between the groups. Study 3 – No statistically significant decreases in squat jump height occurred across the season, although a moderate practical decline occurred following the pre-season. The correlation between training load and squat jump height were statistically non-significant, while the crosscorrelation was significant. The athlete monitoring program was successful in managing the athletes’ neuromuscular fatigue across the season as evidenced by the maintenance of squat jump height and positive relationship between training load and changes in squat jump height. Coach education on the importance of athlete training load and fatigue monitoring is imperative. Collaboration between coaching and sport science staffs in conjunction with an athlete monitoring program can ensure variation in training load and can help manage athlete fatigue across a competitive season

The Pull to Knee-Proper Biomechanics for a Weightlifting Movement Derivative

The pull to knee is an exercise that allows an athlete to become efficient in producing force with an overload stimulus, as well as it is a teaching modality for the initial pull from the floor in weightlifting. This movement emphasizes the precursor movement leading into the double knee bend position

Global Positioning System Analysis of a High School Football Scrimmage

The purpose of this study was to examine the physical demands of a high school American football scrimmage. Male high school football players (N = 25) participated in a spring scrimmage. Global positioning system data and game film were recorded throughout the entirety of the scrimmage to determine the total distance covered, the distance covered in different velocity bands, the number of accelerations and decelerations performed, and the work-to-rest ratio of the scrimmage. The athletes were divided into 2 groups: linemen (L) (N = 7) vs. nonlinemen (NL) (N = 8) for statistical analysis, and independent T-tests with Holm\u27s sequential Bonferroni adjustment were used to determine differences in movement characteristics between the L and NL groups. Average play duration was 5.7 ± 2.1 seconds, whereas the rest interval was 33.4 ± 13.6 seconds between plays, for an overall exercise-to-rest ratio of 1:5.9. Total distance, standing and walking distance, running distance, striding distance, sprinting distance, and total high-speed running distance covered by NL was greater than L (statistically significant at p ≤ 0.05). Distances traveled in each velocity band by position and by play are also included to provide context of our findings. Data from the present study add to the pool of support for the use of position-specific training in preparing high school football players for competition

A Reevaluation Of The Assignment Of The Vibrational Fundamentals And The Rotational Analysis Of Bands In The High-resolution Infrared Spectra Of Trans- And Cis-1,3,5-hexatriene

Assignments of the vibrational fundamentals of cis- and trans-1,3,5-hexatriene are reevaluated with new infrared and Raman spectra and with quantum chemical predictions of intensities and anharmonic frequencies. The rotational structure is analyzed in the high-resolution (0.0013-0.0018 cm(-1)) infrared spectra of three C-type bands of the trans isomer and two C-type bands of the cis isomer. The bands for the trans isomer are at 1010.96 cm(-1) (v(14)), 900.908 cm(-1) (v(16)), and 683.46 cm(-1) (v(17)). Ground state (GS) rotational constants have been fitted to the combined ground state combination differences (GSCDs) for the three bands of the trans isomer. The bands for the cis isomer are at 907.70 cm(-1) (v(33)) and 587.89 cm(-1) (v(35)). GS rotational constants have been fitted to the combined GSCDs for the two bands of the cis isomer and compared with those obtained from microwave spectroscopy. Small inertial defects in the GSs confirm that both molecules are planar. Upper state rotational constants were fitted for all five bands