Influence of temperature on host location and multisensory orientation in the parasitoid Pimpla turionellae (L.) (Hymenoptera)

The ichneumonid wasp Pimpla turionellae (L.) (Hymenoptera) is a specialist parasitoid of lepidopteran pupae, and has to overcome the challenge of reduced chemical and visual cues as pupae are immobile, do not feed and do not emit excrements. Certain hymenopteran species have developed a particular mechanosensory mechanism in order to locate hosts hidden in hollow spaces inside of plant material (Broad & Quicke 2000). Similar to echolocation they use self-produced vibrations, instead of sounds, that are transmitted by the antennae onto the substrate. In analogous way to acoustics, this mechanosensory mechanism is referred to as vibrational sounding (Wäckers & al. 1998). Thermal dependence is well known in acoustical and vibrational communication of arthropods (e.g. Pires & Hoy 1992, Shimizu & Barth 1996) and is likewise presumed to affect mechanosensory host location by vibrational sounding. The species P. turionellae has recently been found to use vibrational sounding successfully in a temperature range from 8 to 28°C, but with less performance of searching behaviour and an adjusted signal production at extreme temperatures (Kroder & al. 2006, & al. 2007b, Samietz & al. 2006). Many insects have evolved strategies to maintain a balance of body temperature by ecto- and endothermic means. Raising and maintaining body temperatures above the ambient environment by endothermic means is particularly known in several hymenopteran species (Heinrich 1993). In the case of a thermally influenced host location mechanism, such means of thermoregulation could be supposed as well in order to maintain performance with changing temperatures. The study elucidates if the wasps are able to regulate their body temperature at suboptimal conditions during vibrational sounding and furthermore examines the role of vibrational sounding in multisensory orientation at different ambient temperatures.Die Schlupfwespe Pimpla turionellae parasitiert versteckte Lepidopterenpuppen und orientiert sie sich bei der Wirtssuche multisensorisch mittels visueller Reize und aktiver Vibrationsortung mit selbst produzierten Schwingungen (Vibrational-Sounding). Die Studie untersucht, inwieweit die Wespen bei Änderung der Umgebungstemperaturen von 8-26°C (1) zwischen der temperatur-sensitiven vibratorischen und der -insensitiven visuellen Orientierung wechseln und (2) gegebenenfalls selbst die Körpertemperatur regulieren können, um die sehr präzise Vibrationsortung bei niedrigen Temperaturen aufrechtzuerhalten. Messungen mit Infrarot-Thermographie zeigen, dass suchende Wespen leicht erhöhte Körpertemperaturen während der vibratorischen Wirtssuche aufweisen, welche auf metabolische Wärmeproduktion zurückzuführen sind. Wahlexperimente unter kontrollierten Temperaturen zeigen zudem, dass die Nutzung der temperatur-sensitiven vibratorischen Reize bei pessimalen Temperaturen abnimmt und die Wespen auf fast ausschließliche visuelle Orientierung wechseln. Folglich wird die Relevanz einzelner Reize bei der multisensorischen Orientierung direkt vom Faktor Temperatur beeinflusst. Solange ein zuverlässiger Reiz vorhanden ist, nimmt dabei auch die Präzision der Lokalisation insgesamt nicht ab

Temperature affects interaction of visual and vibrational cues in parasitoid host location

Parasitoid host location in nature is facilitated by simultaneously using different information sources. How multisensory orientation on the same spatial scale is influenced by environmental conditions is however poorly understood. Here we test whether changes in reliability of cues can cause parasitoids to alter multisensory orientation and to switch to cues that are more reliable under extreme temperatures. In the ichneumonid wasp Pimpla turionellae, multisensory use of thermally insensitive vision and thermally sensitive mechanosensory host location by vibrational sounding (echolocation on solid substrate) was investigated with choice experiments on plant-stem models under optimum temperature (18°C), at high- (28°C) and low-temperature limits (8°C) of vibrational sounding. Temperature affected relative importance of vibrational sounding whereas visual orientation did not vary. At 18°C, parasitoids used visual and vibrational cues with comparable relative importance. At 8 and 28°C, the role of vibrational sounding in multisensory orientation was significantly reduced in line with decreased reliability. Wasps nearly exclusively chose visual cues at 8°C. The parasitoids switch between cues and sensory systems depending on temperature. As overall precision of ovipositor insertions was not affected by temperature, the parasitoids fully compensate the loss of one cue provided another reliable cue is available on the same spatial scal

DC-DC 4-Switch Buck-Boost Converter for Energy Harvesting from Elliptical Machines

The senior project report documents the process of replacing the network of resistors inside an elliptical machine with a DC-DC buck-boost converter. The buck-boost DC-DC converter accepts a wide input range of 5-60 Volts, with an output of 36 Volts for the most efficient use of the already available microinverter. The microinverter reclaims the lost energy and safely distributes it back to the electrical grid. The addition of this project reduces heat emissions from wasted energy and shrinks the carbon footprint of its users

Efficiency of vibrational sounding in parasitoid host location depends on substrate density

Parasitoids of concealed hosts have to drill through a substrate with their ovipositor for successful parasitization. Hymenopteran species in this drill-and-sting guild locate immobile pupal hosts by vibrational sounding, i.e., echolocation on solid substrate. Although this host location strategy is assumed to be common among the Orussidae and Ichneumonidae there is no information yet whether it is adapted to characteristics of the host microhabitat. This study examined the effect of substrate density on responsiveness and host location efficiency in two pupal parasitoids, Pimpla turionellae and Xanthopimpla stemmator (Hymenoptera: Ichneumonidae), with different host-niche specialization and corresponding ovipositor morphology. Location and frequency of ovipositor insertions were scored on cylindrical plant stem models of various densities. Substrate density had a significant negative effect on responsiveness, number of ovipositor insertions, and host location precision in both species. The more niche-specific species X. stemmator showed a higher host location precision and insertion activity. We could show that vibrational sounding is obviously adapted to the host microhabitat of the parasitoid species using this host location strategy. We suggest the attenuation of pulses during vibrational sounding as the energetically costly limiting factor for this adaptatio

Host Location of a Pupal Parasitoid in a Tritrophic System Compared to a Model Offering Mechanosensory Cues Only

Several species of hymenopteran parasitoids are able to locate concealed pupal hosts by vibrational sounding. However, the specific role of this technique of mechanosensory host location has not yet been investigated in a natural, tritrophic system with multiple cues. Here we compared the host location of the pupal parasitoid Xanthopimpla stemmator in a tritrophic system with corn borer pupae in maize stem to the behavior on a paper model offering mechanosensory cues only. In general, the behavioral pattern and the behavioral states exhibited by host-searching female parasitoid were identical in the model and in the tritrophic system, while quantitative aspects differed. Our results demonstrate that vibrational sounding maintains its significance for host location in an environment with multiple cues, and that additional cues may increase responsiveness of female

Visual Orientation of Overwintered Anthonomus pomorum (Coleoptera: Curculionidae)

Vision is considered to be an important component of the sensory system used by herbivorous insects to locate host plants. We investigated preferences for transmissive colors and contrasts in the apple blossom weevil, Anthonomus pomorum L., which colonizes apple trees, Malus domestica Borkh., in early spring. The main components of the supposed visual system, green, blue, and UV light, were offered to the weevils versus black or versus each other in a dual choice arena. Furthermore, silhouettes of two different achromatic contrasts were offered in front of the colors blue and green. We also tested whether visual preferences change with time, i.e., 2, 4, and 6 d after termination of diapause in early spring. Female weevils chose UV, green, and blue over black, whereas males only chose UV over black in a series of dual choice experiments. Both sexes preferred blue to green. Weevil response to silhouettes of different contrasts was influenced by the background color, i.e., the silhouette with the higher contrast was preferred in front of green, whereas the silhouette with the lower contrast was preferred in front of blue. The preferences during visual orientation did not change with time after diapause. The more discriminating response of females may reflect a greater visual acuity or a greater visual responsiveness in females than in males because of a higher affinity for the host plant. Our findings indicate that weevils might have a trichromatic visual system and that they are able to include visual cues for the location of host plants in the fiel

Efficiency of vibrational sounding in parasitoid host location depends on substrate density

ISSN:0340-7594ISSN:1432-135

Ambient temperature affects mechanosensory host location in a parasitic wasp

Certain parasitic wasps (Ichneumonidae, Pimplinae) use self-produced vibrations transmitted on plant substrate to locate their immobile concealed hosts (i.e. lepidopteran pupae). This mechanosensory mechanism, called the vibrational sounding, depends both on physical cues of the environment and physical activity of the parasitoid and is postulated to depend on ambient temperature. We analysed the influences of temperature on vibrational sounding by choice experiments using plant-stem models with hidden host mimics in the temperate species Pimpla turionellae. The results show a significant effect of temperature on host-location activity and on the success of this process. Outside an optimum range, the performance of the wasps decreased both at low and high temperatures. Below 10°C and beyond 24°C, the wasps displayed (1) substantial reduction in responsiveness, i.e. proportion of females showing ovipositor insertions, (2) reduction of quantitative activity with ovipositor insertions in the individuals, and (3) reduced precision of mechanosensory host location. Nevertheless, female wasps were able to locate their host over a surprisingly broad range of ambient temperatures which indicates that the wasps are able to compensate for temperature effects on vibrational soundin

Perception of Chromatic Cues During Host Location by the Pupal Parasitoid Pimpla turionellae (L.) (Hymenoptera: Ichneumonidae)

Chromatic and achromatic plant cues are expected to be particularly important for parasitoids of endophytic pupal hosts, because these stages do not feed and therefore avoid volatile emission caused by plant tissue damage. Endophytic feeding can cause discoloration or desiccation, leading to changes in color and/or brightness of infested plant parts that may be visually detected by parasitoids. The role of color cues in the host-finding behavior of parasitoids is poorly understood, and the visual system of most parasitoid species has not yet been investigated. We studied color discrimination ability and innate color preferences in the pupal parasitoid Pimpla turionellae (L.) (Hymenoptera: Ichneumonidae) during location of concealed hosts. Responses to combinations of yellow and blue bands of different reflectance intensities were investigated on cylindrical models of plant stems. The parasitoid's reaction to these chromatic cues was evaluated by scoring the number of ovipositor insertions into the colored bands. Female parasitoids discriminated blue from yellow irrespective of total reflectance and inserted their ovipositors significantly more often into the blue area. True color vision is demonstrated for the examined species, and responses to chromatic cues are discussed in relation to their importance for host location in parasitoids. Results of this study and of our previous work suggest that P. turionellae uses contrasts (chromatic or achromatic) rather than specific color characteristics in visual host locatio

Implications of changes in seasonal mean temperature for agricultural production systems: three case studies

- The performance of dairy cows will suffer from elevated temperatures, reflecting the extent and uncertainty of projected warming in different scenarios, with a marked increase in heat stress for non-intervention scenarios (A1B and A2) toward the end of the century. This calls for the adoption of protective measures in the management of indoor and outdoor animal environments. - A substantial risk of a prolonged pest control season for the codling moth (an apple pest) is projected toward the end of the century for Northern Switzerland sites, and mid-century for the Ticino. Timely preventive programs are anticipated to represent a key ingredient of adaptation to changing risks from agricultural pests. - Results suggest that in the near future viticulture could benefit from increasing temperatures as a wider range of grape varieties could be grown. Toward the end of the century negative impacts from extreme temperatures are nevertheless expected to become important