Quince peel polyphenolic extract blocks human colon adenocarcinoma LS174 cell growth and potentiates 5-fluorouracil efficacy

Additional file: Figure S1. The effect of combination of Peph phenolic compounds on proliferation of LS174 cells compared to total Peph extract. Human colon adenocarcinoma LS174 cells were treated for 72 h with different combinations (A. 3 combinations, B. 4 combinations, C. 5 combinations) of Peph phenolic compounds. All compounds where tested at equivalent concentrations to that present in 5 Οg/ml of the total peel polyphenolic extract. Cell viability was determined by MTT assay

Retroperitoneal Abscess: A Rare Localization of Tubercular Infection

Incidence of tuberculosis infection has considerably increased during the past 20 years due to the HIV pandemic and continues to be one of the most prevalent and deadly infections worldwide. Extrapulmonary tuberculosis lacks specific clinical manifestation and can mimic many diseases. It can invade neighbouring tissue and form a big cyst with manifesting clinical symptoms. We describe a rare case of 31-year-old immunocompetent man affected by a retroperitoneal abscess secondary to tubercular infection. Exploratory laparotomy and histopathological examinations of tissue were required for achieving diagnosis of tuberculosis. No pulmonary or spinal involvement was identified. The patient was successfully treated with standard four-drug antitubercular therapy

EOS workshop

EOS provides a very detailed log system which provides useful information of all the user and system operations that are performed at any time. Each EOS daemon has its own log file and tracing operations that involve different components can be a time consuming task (MGM -> FST1 -> FST2). With Grafana Loki and Promtail, we setup a logging aggregation system that allows tracing operations across different EOS components from a central and unified interface, considerably reducing the time needed for debugging sessions or usage patterns investigation

Oncocytoma with a vascular extension, associated with a papillary carcinoma: A case report

This is a case report about a patient which presents with two right renal tumors, one of them being an oncocytoma with typical histopathological features and renal vein extension. Recent studies show that despite renal vein thrombus being a histological sign of malignancy; when associated with renal oncocytoma, it should not alter the benign prognosis of oncocytoma, and a simple follow-up may be carried. Further explorations should be done when easily available, and when the histopathologic diagnosis of oncocytoma is uncertain, to rule out the differential diagnosis of a chromophobe renal cell carcinoma, oncocytic variant

Biodegradation of fluoranthene by a newly isolated strain of Bacillus stratosphericus from Mediterranean seawater of the Sfax fishing harbour, Tunisia

International audienceA physico-chemical characterization of seawater taken from the fishing harbour of Sfax, Tunisia, revealed a contamination by organic and inorganic micropollutants. An aerobic marine halotolerant Bacillus stratosphericus strain FLU5 was isolated after enrichment on fluoranthene, a persistent and toxic polycyclic aromatic hydrocarbon (PAH). GC-MS analyses showed that strain FLU5 was capable of degrading almost 45 % of fluoranthene (100 mg l−1), without yeast extract added, after 30 days of incubation at 30 g l−1 NaCl and 37 °C. In addition, the isolate FLU5 showed a remarkable capacity to grow on a wide range of aliphatic, aromatic and complex hydrocarbons. This strain could also synthesize a biosurfactant which was capable of reducing the surface tension of the cell-free medium, during the growth on fluoranthene. The biodegradative abilities of PAHs are promising and can be used to perform the bioremediation strategies of seawaters and marine sediments contaminated by hydrocarbons

Inflammatory suppurated tumor of urachus mimicking a malignant urachus adenocarcinoma

This is a case report about a patient presenting with a urachal mass mimicking a urachus adenocarcinoma. Cystoscopy showed a vesicourachal patent diverticulum. Histological findings after the removal of the umbilicus, urachus, urachal tumor, as well as a bladder cuff, consisted of a nonspecific polymorphous suppurative inflammatory infiltrate. Urachal adenocarcinoma is an aggressive tumor with poor prognosis if not treated while it is still localized. Surgical excision is the only recommended treatment that offers the best chances of survival. As no preoperative procedure has been proven accurate enough to rule out the diagnosis of adenocarcinoma, surgery appears to be inevitable

Isolation and characterization of a newly naphthalene degrading Halomonas pacifica, strain Cnaph3: biodegradation and biosurfactant production studies

A newly marine Halomonas pacifica strain Cnaph3 was isolated, as a naphthalene degrader and biosurfactant producer, from contaminated seawater collected in Ataya's fishing harbor, located in Kerkennah Islands, Tunisia. Chromatography flame ionization detector analysis revealed that 98.8% of naphthalene (200 mg/L) was degraded after 7 days of incubation, at 30 g/L NaCl and 37 C. Strain Cnaph3 showed also a noticeable capacity to grow on a wide range of aliphatic, aromatic, and complex hydrocarbons. Interestingly, strain Cnaph3 showed a significant potential to produce biosurfactants in the presence of all tested substrates, particularly on glycerol (1%, v/v). Electrospray ionization analysis of the biosurfactant, designated Bios-Cnaph3, suggested a lipopeptide composition. The critical micelle concentration of Bios-Cnaph3 was about 500 mg/L. At this concentration, the surface tension of the water was reduced to 27.6 mN/m. Furthermore, Bios-Cnaph3 displayed interesting stabilities over a wide range of temperatures (4-105 C), salinities (0-100 g/L NaCl), and pH (2.2-12.5). In addition, it showed promising capacities to remove used motor oil from contaminated soils. The biodegradation and biosurfactant-production potential of the Halomonas sp. strain Cnaph3 would present this strain as a favorite agent for bioremediation of hydrocarbon-contaminated sites under saline conditions

Isolated ureteral injury from multiple stab wounds: A case report

This is a case report about a 44-year-old woman who sustained multiple stab wounds, resulting in an isolated right ureteral injury. Despite a precise diagnosis from an urgent CT scan showing contrast material extravasation, the patient's stable vital signs allowed for a thorough surgical exploration, leading to an accurate diagnosis. Immediate surgical repair of the right ureter using an end-to-end anastomosis following precise surgical principles achieved complete restoration of the initial injuries. The case highlights the accuracy of CT scan assessment, the unreliability of hematuria in diagnosing ureteral injuries, and the need for a high index of suspicion during surgical exploration to avoid overlooking such cases

Production, characterization and biotechnological potential of lipopeptide biosurfactants from a novel marine Bacillus stratosphericus strain FLU5

This work aimed to study the potential of a newly marine bacterium, Bacillus stratosphericus FLU5, to produce an efficient surface active agent BS-FLU5. Biosurfactant production was examined on different carbon sources using the surface tension measurement and the oil displacement test. Strain FLU5 showed its capacity to produce biosurfactants on the most of tested substrates and in particular the residual frying oil, which is an alternative, cheap and renewable carbon source, thus minimizing the high cost of producing surfactants. MALDI-TOF MS/MS analyses confirmed the presence of lipopeptides identified as members of surfactin and pumilacidin isomers. The critical micellar concentration of the purified lipopeptides produced by strain FLU5 was 50 mg/l and, at this concentration, the surface tension of the water was reduced from 72 to 28 mN/m. Furthermore, the crude lipopeptides showed interest stability against a broad range of pH (2.1-12), temperature (10-121 °C) and salinity (0-120 g/l NaCl). The biosurfactant BS-FLU5 demonstrated negligible cytotoxic effect against mammalian cells (HEK293 human embryonic kidney cell line) at all of tested concentration (125-1000 µg/ml). The application of BS-FLU5 in oil recovery from soil contaminated by hydrocarbons ( used motor oil) showed that it was more effective on the hydrocarbon-remobilization than some tested synthetic surfactants. These results highlight the applicability of the lipopeptides produced by the new marine Bacillus stratosphericus strain FLU5 in different fields, especially in environmental remediation processes. Production of lipopeptides is a characteristic of several Bacillus species, but to the best of our knowledge, this is the first report showing the potential of Bacillus stratosphericus for efficient production of biosurfactants or lipopeptides