10,486 research outputs found
Knee kinematics of total knee replacement patients: pre and post operative analysis using computer generated images
This project aims to show a comparison of knee kinematics in pre- and post-operative knee replacement surgery, using computer animation to represent a patient specific model of the knee joint interactions under every day conditions
Toolkit for gender analysis of crop and livestock production, technologies and service provision
Opportunities for promoting gender equality in rural Ethiopia through the commercialization of agriculture
Rural women in Ethiopia represent a tremendous productive resource in the agricultural sector. They are major contributors to the agricultural workforce, either as family members or in their own right as women heading households. However, despite recent policy initiatives to strengthen the position of women in the agricultural sector, a mixture of economic constraints, cultural norms and practices continue to limit their contribution to household food security and, to a lesser extent, inhibits the commercialization of the sector. Gender roles and relationships influence the division of work, the use of resources, and the sharing of the benefits of production between women and men. In particular, the introduction of new technologies and practices, underpinned by improved service provision, often disregards the gendered-consequences of market-oriented growth and many benefits bypass women. Not only do these circumstances have implications for issues of equality but also may be detrimental to the long-term sustainability of development initiatives. Despite the crucial role of the agricultural sector in the Ethiopian economy, studies on gender aspects of agricultural commercialization are relatively scarce. The main purpose of this paper is to contribute to the knowledge base about implications of gender roles and responsibilities for the development of the agricultural sector. This paper discusses gender issues in the context of the Improving Productivity and Market Success (IPMS) of Ethiopian Farmers’ Project being implemented by the International Livestock Research Institute (ILRI) and the Ethiopian Ministry of Agriculture and Rural Development. The findings are based on qualitative studies undertaken by the IPMS gender research team and Research and Development Officers in 10 pilot learning woredas (PLWs) located in 4 regions of the country. The study had three objectives: to increase the understanding of the different roles of women and men in agricultural activities, marketing and decision making, and their share in the benefits; to identify potential barriers for women’s and men’s participation in market-led development initiatives and technology adoption; and to identify what actions may overcome some of these barriers
Evaluation of mTOR-regulated mRNA translation.
mTOR, the mammalian target of rapamycin, regulates protein synthesis (mRNA translation) by affecting the phosphorylation or activity of several translation factors. Here, we describe methods for studying the impact of mTOR signalling on protein synthesis, using inhibitors of mTOR such as rapamycin (which impairs some of its functions) or mTOR kinase inhibitors (which probably block all functions).To assess effects of mTOR inhibition on general protein synthesis in cells, the incorporation of radiolabelled amino acids into protein is measured. This does not yield information on the effects of mTOR on the synthesis of specific proteins. To do this, two methods are described. In one, stable-isotope labelled amino acids are used, and their incorporation into new proteins is determined using mass spectrometric methods. The proportions of labelled vs. unlabeled versions of each peptide from a given protein provide quantitative information about the rate of that protein's synthesis under different conditions. Actively translated mRNAs are associated with ribosomes in polyribosomes (polysomes); thus, examining which mRNAs are found in polysomes under different conditions provides information on the translation of specific mRNAs under different conditions. A method for the separation of polysomes from non-polysomal mRNAs is describe
Higher biomass accumulation by increasing phosphoribosylpyrophosphate synthetase activity in Arabidopsis thaliana and Nicotiana tabacum
Plants are able to produce all the organic compounds required for development and growth. As developmental processes and metabolic pathways use a common resource pool, the tight regulation of the distribution of metabolites between growth, production of defence compounds and storage products can be assumed. A transgenic approach was used to investigate the importance of supplying the key intermediate phosphoribosylpyrophosphate (PRPP) for plant growth and biomass accumulation in the model plant Arabidopsis thaliana and in Nicotiana tabacum. For this purpose, the Ashbya gossypii genes coding for either PRPP synthetase (PRS) or a mutated variant of the same gene were over-expressed under the control of a constitutive promoter. It was shown that increased PRS activity in A. thaliana or N. tabacum leads to a substantial increase in biomass accumulation under different standardized growth conditions. Growth enhancement was accompanied by significant changes in the amount of sugars and other metabolites. This study provides evidence that the supply of PRPP co-limits growth rates, and has obvious implications for biotechnological strategies aiming to increase plant biomass as an alternative renewable energy source
Dynamics of the HIV/AIDS epidemic in value chain development in rural Ethiopia and responses through market-led agricultural initiatives
This paper discusses the rural HIV epidemic in the context of the Improving Productivity and Market Successes (IPMS) of Ethiopian Farmers' Project being implemented by the International Livestock Research Institute and the Ministry of Agriculture and Rural Development. Its purpose is to contribute to the knowledge base about HIV/AIDS in the agricultural sector by, firstly, increasing understanding about the HIV/AIDS in the context of rural communities and, secondly, identifying opportunities to minimize the risk of HIV infection and mitigate the impacts of AIDS in rural communities through market-led agricultural initiatives A fieldwork was conducted to examine three questions: What are the sources of risk of HIV infection and who is at risk? To what extent does AIDS already impact rural communities and their livelihood systems? What might a production and market-oriented project, such as IPMS, offer to address the epidemic? The first section of this working paper is introduction. The second section examines three sources of risk of infection associated with the urban hinterland, the movement of people into and from rural communities, and cultural traditions and practices within rural communities, and concludes with a summary of risk by person. The third section draws on field experience from the PLWs (Pilot Learning Woreda) to demonstrate the potential effect of market-led agricultural growth on hastening the spread of HIV by examining six principal commodity value chains, namely cereals, oilseeds, spices, vegetables, coffee and livestock. The extent to which AIDS has already impacted the rural communities in the 10 PLWs is examined in section four, and sources of vulnerability to future impacts of AIDS are identified. The final section summarizes the main implications of the disease for IPMS and discusses a range of opportunities for reducing the risk of HIV infection and mitigating the impacts of AIDS by improving agricultural productivity and market linkages. They are illustrated with examples of innovative approaches that have been developed, field-tested and implemented by IPMS in several woredas, often in partnership with other organizations
Reliable microsatellite genotyping of the Eurasian badger (Meles meles) using faecal DNA
The potential link between badgers and bovine tuberculosis has made it vital to develop
accurate techniques to census badgers. Here we investigate the potential of using genetic
profiles obtained from faecal DNA as a basis for population size estimation. After trialling
several methods we obtained a high amplification success rate (89%) by storing faeces in
70% ethanol and using the guanidine thiocyanate/silica method for extraction. Using 70%
ethanol as a storage agent had the advantage of it being an antiseptic. In order to obtain reliable
genotypes with fewer amplification reactions than the standard multiple-tubes
approach, we devised a comparative approach in which genetic profiles were compared
and replication directed at similar, but not identical, genotypes. This modified method
achieved a reduction in polymerase chain reactions comparable with the maximumlikelihood
model when just using reliability criteria, and was slightly better when using
reliability criteria with the additional proviso that alleles must be observed twice to be considered
reliable. Our comparative approach would be best suited for studies that include
multiple faeces from each individual. We utilized our approach in a well-studied population
of badgers from which individuals had been sampled and reliable genotypes obtained.
In a study of 53 faeces sampled from three social groups over 10 days, we found that direct
enumeration could not be used to estimate population size, but that the application of
mark–recapture models has the potential to provide more accurate results
Gender and HIV/AIDS mainstreaming in a market-oriented agricultural development context: a training manual for frontline staff
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