High Order Asymptotic Preserving and Classical Semi-implicit RK Schemes for the Euler-Poisson System in the Quasineutral Limit

In this paper, the design and analysis of high order accurate IMEX finite volume schemes for the compressible Euler-Poisson (EP) equations in the quasineutral limit is presented. As the quasineutral limit is singular for the governing equations, the time discretisation is tantamount to achieving an accurate numerical method. To this end, the EP system is viewed as a differential algebraic equation system (DAEs) via the method of lines. As a consequence of this vantage point, high order linearly semi-implicit (SI) time discretisation are realised by employing a novel combination of the direct approach used for implicit discretisation of DAEs and, two different classes of IMEX-RK schemes: the additive and the multiplicative. For both the time discretisation strategies, in order to account for rapid plasma oscillations in quasineutral regimes, the nonlinear Euler fluxes are split into two different combinations of stiff and non-stiff components. The high order scheme resulting from the additive approach is designated as a classical scheme while the one generated by the multiplicative approach possesses the asymptotic preserving (AP) property. Time discretisations for the classical and the AP schemes are performed by standard IMEX-RK and SI-IMEX-RK methods, respectively so that the stiff terms are treated implicitly and the non-stiff ones explicitly. In order to discretise in space a Rusanov-type central flux is used for the non-stiff part, and simple central differencing for the stiff part. AP property is also established for the space-time fully-discrete scheme obtained using the multiplicative approach. Results of numerical experiments are presented, which confirm that the high order schemes based on the SI-IMEX-RK time discretisation achieve uniform second order convergence with respect to the Debye length and are AP in the quasineutral limit

'Template-free' hierarchical MoS<inf>2</inf>foam as a sustainable 'green' scavenger of heavy metals and bacteria in point of use water purification

Molybdenum disulfide (MoS2), with its unique optical and electrical properties, has been explored for a variety of applications in the recent past. Still, its capabilities in point-of-use heavy metal ion removal remain to be explored. Herein, for the first time using a facile approach, we fabricated three-dimensional (3D) MoS2 foam from exfoliated single to few-layered MoS2 sheets for the selective exclusion of heavy metals and stringent bactericidal response. This foam was able to exclude 99.9% of Pb(ii) and 98.7% of As(iii) instantaneously and reduced more than 98% of bacteria E. coli. Moreover, the foam exhibits selective toxicity towards bacterial cells while showing no observable toxicity towards mammalian cells. The foam can be recycled and reused for at least five cycles under accelerated conditions and thus can be used for a promising non-cytotoxic, facile, and environmentally benign process for inline water remediation to remove heavy metal ions from the feed and as a potential antibacterial agent

Antagonistic activity of cellular components of Pseudomonas species against Aeromonas hydrophila

Antagonistic effects of Pseudomonas fluorescens, P. aeruginosa and P. putida were studied against 12 strains of Aeromonas hydrophila (Ah1–Ah12). Four different fractions of cellular component (i.e. whole cell product, heat killed whole cell product, intra cellular product and extra cellular product) of all Pseudomonas species were equally effective in reducing growth of A. hydrophila strains, as measured by the zone of inhibition in an in vitro sensitivity test and have potential action against A. hydrophila infection in fishes

Prevalence of HIV Drug Resistance Mutations in HIV Type 1 Isolates in Antiretroviral Therapy Naïve Population from Northern India

Objective. The increased use of antiretroviral therapy (ART) has reduced the morbidity and mortality associated with HIV, adversely leading to the emergence of HIV drug resistance (HIVDR). In this study we aim to evaluate the prevalence of HIVDR mutations in ART-naive HIV-1 infected patients from northern India. Design. Analysis was performed using Viroseq genotyping system based on sequencing of entire protease and two-thirds of the Reverse Transcriptase (RT) region of pol gene. Results. Seventy three chronic HIV-1 infected ART naïve patients eligible for first line ART were enrolled from April 2006 to August 2008. In 68 patients DNA was successfully amplified and sequencing was done. 97% of HIV-1 strains belonged to subtype C, and one each to subtype A1 and subtype B. The overall prevalence of primary DRMs was 2.9% [2/68, 95% confidence interval (CI), 0.3%–10.2%]. One patient had a major RT mutation M184V, known to confer resistance to lamivudine, and another had a major protease inhibitor (PI) mutation D30N that imparts resistance to nelfinavir. Conclusion. Our study shows that primary HIVDR mutations have a prevalence of 2.9% among ART-naive chronic HIV-1 infected individuals

Synthesis of palladium complexes derived from amido linked N-heterocyclic carbenes and their use in Suzuki cross coupling reactions

One of the authors (RSC) is grateful to DST for the financial support under the DST young scientist scheme YSS/2014/000797.Treatment of 1-(n-butyl)-3-N-(2-Ar)acetamido-1, 3-imidazolium chloride (Ar=furylmethyl,phenylmethyl) with excess K2CO3 and [PdCl2(L−L)] (L−L=2 PPh3, dppf) afforded orange compounds of composition [(1-(n-butyl)-3-N-(2-Ar)acetamido-1,3-imidazol-2-ylidene)]2Pd (Ar=furylmethyl; phenylmethyl). These complexes were characterized by NMR (1H and 13C{1H} NMR), IR and micro-analysis data. Subsequently, the catalytic efficiency of these complexes for cross coupling reactions between 4-haloarenens (halo=Br, I) and phenylboronic acid was studied under different solvents (acetonitrile, THF and DMF), temperatures with different catalyst loadings. The molecular structure of [(1-(n-butyl)-3-N-(2-furylmethyl)acetamido-1, 3-imidazol-2-ylidene)]2Pd was established by single crystal X-ray diffraction analysis.PostprintPeer reviewe

Correlation between CD4 counts of HIV patients and enteric protozoan in different seasons – An experience of a tertiary care hospital in Varanasi (India)

<p>Abstract</p> <p>Background</p> <p>Protozoan infections are the most serious among all the superimposed infections in HIV patients and claim a number of lives every year. The line of treatment being different for diverse parasites necessitates a definitive diagnosis of the etiological agents to avoid empirical treatment. Thus, the present study has been aimed to elucidate the associations between diarrhoea and CD4 counts and to study the effect of HAART along with management of diarrhoea in HIV positive patients. This study is the first of its kind in this area where an attempt was made to correlate seasonal variation and intestinal protozoan infestations.</p> <p>Methods</p> <p>The study period was from January 2006 to October 2007 wherein stool samples were collected from 366 HIV positive patients with diarrhea attending the ART centre, inpatient department and ICTC of S.S. hospital, I.M.S., B.H.U., Varanasi. Simultaneously, CD4 counts were recorded to assess the status of HIV infection vis-à-vis parasitic infection. The identification of pathogens was done on the basis of direct microscopy and different staining techniques.</p> <p>Results</p> <p>Of the 366 patients, 112 had acute and 254 had chronic diarrhea. The percentages of intestinal protozoa detected were 78.5% in acute and 50.7% in chronic cases respectively. Immune restoration was observed in 36.6% patients after treatment on the basis of clinical observation and CD4 counts. In 39.8% of HIV positive cases <it>Cryptosporidium </it>spp. was detected followed by <it>Microsporidia </it>spp. (26.7%). The highest incidence of intestinal infection was in the rainy season. However, infection with <it>Cyclospora </it>spp. was at its peak in the summer. Patients with chronic diarrhea had lower CD4 cell counts. The maximum parasitic isolation was in the patients whose CD4 cell counts were below 200 cells/μl.</p> <p>Conclusion</p> <p>There was an inverse relation between the CD4 counts and duration of diarrhea. <it>Cryptosporidium </it>spp. was isolated maximum among all the parasites in the HIV patients. The highest incidence of infection was seen in the rainy season.</p