Physicochemical and antigenic properties correlation in Streptococcus gordonii vaccine vectors and development of a Streptococcus pneumoniae intra-tracheal mouse model of pneumonia

In the present thesis, the correlation between the physicochemical and antigenic properties of different recombinant Streptococcus gordonii vaccine vectors was studied. Evaluation of vaccine efficacy, antigenicity and immugenicity is a crucial step in developing vaccines, thus investigating a simple method to analyze vaccine efficacy besides other methods could be a major part of developing bacterial vaccine vectors. To approach this, isoelectric point measurements and zeta-potential titration as well as antigenicity and immunogenicity of S.gordonii vaccine vectors (with fbpA, RPS, gtfg genes mutations expressing H1 antigen) were used. These data showed that strains with more positive surface charge had higher heterologous antigen recognition and lower antibody responses in the serum of immunized mice. This correlation between surface charge and antigenicity and immunogenicity revealed the importance of using simple methods such as zeta potential titration and isoelectric point measurements to predict engineered vaccine vectors antigenicity and possible efficacy. In the second part of the thesis the immune recall in the days following Streptococcus pneumoniae lung infection by transcriptomic analysis was studied. S. pneumoniae is the most common bacterial cause of community-acquired pneumonia. Host-pathogen interaction is poorly understood, and factors that drive a more severe phenotype are unknown. One way to study host response to pathogen is using the stimulation of immune system cells with live or killed bacteria. We combined transcriptomic and cytokine level analysis on stimulated mouse splenocytes revealing the presence of a recall immune response involving both innate and adaptive immunity, stronger from the fourth day after infection. This model could analyze immune responses involved in pneumococcal infection as well as vaccine and experimental therapies efficacy in future studies. Finally, the development of a S. pneumoniae mouse model of pneumonia by intra-tracheal infection was set up. The nasopharynx of humans is the only natural reservoir for the pneumococci. To mimic human pneumonia, mice models are widely used. Bacteria can be administered to mice intranasally, intratracheally or as aerosols. Pneumococcal pneumonia was induced in mice by intra-tracheal inoculation with different doses of S. pneumoniae TIGR4. Data showed high colonization of bacteria in lung, liver and spleen starting 24 hours post-infection. Pneumonia mortalities were observed in all mice infected by 108 within 24 hours of infection. Further analysis should be done to investigate the host-pathogen interaction as well as vaccine and experimental therapies efficacy by using this model.

Metaphors in Arabic and English Texts: A Case Study of Translation of Metaphors in the English Versions of Al-Sahifah Al-Sajjadiyyah

The aim of the present research is the study of translation of metaphor as one of the literary devices of Al-Sahifah Al-Sajjadiyah from Arabic into English. Al-Sahifah Al-Sajjadiyah contains 54 valuable supplications regarding political, social, military, family, religious, etc, issues. The secret of its permanency among Moslems and Shi'ites, in particular, has been its elegance and fluency. It is replete with literary devices such as proverbs, parables, metaphors, etc. It contains beautiful metaphors throughout. This study is an attempt to examine the translation of this literary device from Arabic into English based on Roman Jakobson’s (1956) framework. Therefore, a group of 65 metaphors of that book was selected from the entire book. The translation of selected metaphors by two English translators, namely William C. Chittick (2008) and Sayyid Ahmad Muhani (1984) were compared with the original ones according to the Roman Jakobson’ s (1956) syntagmatic and paradigmatic axes.On the syntagmatic axis, the axis of combination, words are linked, or chained, together according to grammatical rules, but we make choices about which words to link together on the paradigmatic axis, the axis of selection. The study finally comes up with the conclusion that Chittick has tried to render the metaphors as literally and of course faithfully as possible. He has observed two axes in his translation more than second translator, Muhani and for this reason approaches the original text’s literary style. Muhani mostly has converted the metaphors to their senses. He has more attention to meaning and content rather than linguistic form and in some cases ignoring the form to present the meaning. Keywords: Al-Sahifah Al-Sajjadiyah, Metaphor, Jakobson, Syntagmatic axis, Paradigmatic axis

The Relationship Between Knee Moments and Function with Western Ontario and McMaster Universities in Moderate Knee Osteoarthritis

Introduction: The knee is the most affected weight-bearing joint by osteoarthritis. The kinetics parameters are correlated with the progression of knee osteoarthritis (KOA). This study was done to investigate the relationship between kinetics parameters and functional tests with Western Ontario and McMaster Universities osteoarthritis index (WOMAC) scores in people with moderate KOA. Materials and Methods: Twenty- three participants with moderate KOA participated in this study. Gait analysis involved the measurement of the external peak knee adduction moment (PKAM), peak knee flexion moment (PKFM), knee adduction moment impulse (KAM impulse), and knee flexion moment impulse (KFM impulse) during level walking. Functional tests included timed up and go (TUG) and figure of eight walkings (FO8W) tests. Pearson’s correlation coefficient was used to investigate the correlation between kinetics parameters and functional test scores with WOMAC total scores and sub-scores. Results: There was a significant inverse correlation between the first PKAM and WOMAC total score and pain sub-score (r=-0.43 P=0.03 and r=-0.6 P=0.002, respectively). Also, there was a significant inverse correlation between the second PKAM and pain sub-score (r=-0.46 P=0.02). There was no significant correlation between functional tests and WOMAC scores. Conclusion: The low score of the WOMAC in the moderate KOA should not be attributed to the low level of joint knee moments

Serodiagnosis of fasciolosis by fast protein liquid chromatography-fractionated excretory/secretory antigens

In several studies, different antigenic preparations and diverse immunological tests were applied for serodiagnosis of Fasciola hepatica infections. Most of these preparations showed cross-reactivity with proteins of other parasites. Application of purified antigens might reduce these cross-reactivities. Here, we used fast protein liquid chromatography (FPLC)-fractionated extracts of F. hepatica excretory/secretory antigens (E/S Ags) for serodiagnosis of human and sheep fasciolosis. To develop an improved diagnostic method, we fractionated F. hepatica E/S Ags by anion exchange chromatography on a Sepharose CL-6B column and then tested the serodiagnostic values of the fractions. We used sera from F. hepatica-infected human and sheep as positive controls. Sera from patients with hydatidosis and strongyloidiasis were used for cross-reactivity studies. Enzyme-linked immunosorbent assays (ELISA) of the second FPLC peak, containing 20, 25, and 70 kDa proteins, discriminated between F. hepatica-infected and uninfected human and sheep samples. Fractionation of F. hepatica E/S Ags by FPLC is a fast and reproducible way of obtaining antigens useful for serodiagnosis of human and sheep fasciolosis with acceptable sensitivity and specificity

Simplifying Stochastic Mathematical Models of Biochemical Systems

Stochastic modeling of biochemical reactions taking place at the cellular level has become the subject of intense research in recent years. Molecular interactions in a single cell exhibit random fluctuations. These fluctuations may be significant when small populations of some reacting species are present and then a stochastic description of the cellular dynamics is required. Often, the biochemically reacting systems encountered in applications consist of many species interacting through many reaction channels. Also, the dynamics of such systems is typically non-linear and presents multiple time-scales. Consequently, the stochastic mathematical models of biochemical systems can be quite complex and their analysis challenging. In this paper, we present a method to reduce a stochastic continuous model of well-stirred biochemical systems, the Chemical Langevin Equation, while preserving the overall behavior of the system. Several tests of our method on models of practical interest gave excellent results

Development of a persian version of the children’s fear survey schedule-dental subscale (cfss-ds) among 8-12 year-old female students in Tehran

Background and Aims: Children&rsquo;s Fear Survey Schedule- Dental Subscale (CFSS-DS) is considered as the most popular instrument available to assess dental fear in children. The aim of this study was to develop a Persian version of the CFSS-DS among 8-12 year-old female children resident in Tehran. Materials and Methods: At first, the questionnaire was translated in Farsi and&nbsp; back translated in English afterwards. While, the back translated version was confirmed, we considered the Persian version as the final instrument. Content validity and reliability of the questionnaire was assessed. We used test-retest method to evaluate the reliability, 287 female students aged 8 to 12 from 7 schools in 6th district of education ministry of Tehran were interviewed two times with interval of seven days. In addition, the infraclass correlation coefficient (ICC) and percentage of agreement were calculated. The score of dental fear was measured with range of 13 to 65 and changed to a number between 0 and 100. The relationship between dental fear score and the independent variables including age, history of hospitalization, last medical visit, and history of dental treatment were evaluated using the linear regression models. Results: The mean score of dental fear was calculated 20.52. The score of dental fear was significantly associated with the last medical visit (P=0.04) and past dental history (P<0.001). The percentage of agreement was more than 80% for all the questions. In each question, Kappa was evaluated as moderate to good and ICC was good to very good (more than 0.4). Conclusion: The findings of this study demonstrated that the Persian version of CFSS-DS was valid and reliable among 8-12 year-old children