9 research outputs found
Dermofarmazia. Osasuna kanpoaldetik zaintzea
Get PDF196 p.Aurkibidea:
1. Sarrera.
2. Larruazalaren eta larruazal-eranskinen histologia eta fisiologÃa.
3. Aurpegiaren garbiketa.
4. Aurpegiaren hidratazioa.
5. Aknea.
6. Larruazala zahartzea.
7. Larruazalaren pigmentazioa.
8. Eguzki-babesa.
9. Zelulitisa eta ildoak.
10. Bainurako prestakinak.
11. Ilearen garbiketa.
12. Ilea zaintzeko beste produktu batzuk.
13. Buruko ile-larruaren kosmetika. Ileko arazoak.
14. Zahia eta seborrea.
15. Ahoko higienea.
16. Bizarrerako produktuak.
17. Produktu kosmetikoen kontrolak eta legeria
Dermofarmazia. Osasuna kanpoaldetik zaintzea
Get PDF196 p.Aurkibidea:
1. Sarrera.
2. Larruazalaren eta larruazal-eranskinen histologia eta fisiologÃa.
3. Aurpegiaren garbiketa.
4. Aurpegiaren hidratazioa.
5. Aknea.
6. Larruazala zahartzea.
7. Larruazalaren pigmentazioa.
8. Eguzki-babesa.
9. Zelulitisa eta ildoak.
10. Bainurako prestakinak.
11. Ilearen garbiketa.
12. Ilea zaintzeko beste produktu batzuk.
13. Buruko ile-larruaren kosmetika. Ileko arazoak.
14. Zahia eta seborrea.
15. Ahoko higienea.
16. Bizarrerako produktuak.
17. Produktu kosmetikoen kontrolak eta legeria
Malaria Vaccine Adjuvants: Latest Update and Challenges in Preclinical and Clinical Research
Get PDFThere is no malaria vaccine currently available, and the most advanced candidate has recently reported a modest 30% efficacy against clinical malaria. Although many efforts have been dedicated to achieve this goal, the research was mainly directed to identify antigenic targets. Nevertheless, the latest progresses on understanding how immune system works and the data recovered from vaccination studies have conferred to the vaccine formulation its deserved relevance. Additionally to the antigen nature, the manner in which it is presented (delivery adjuvants) as well as the immunostimulatory effect of the formulation components (immunostimulants) modulates the immune response elicited. Protective immunity against malaria requires the induction of humoral, antibody-dependent cellular inhibition (ADCI) and effector and memory cell responses. This review summarizes the status of adjuvants that have been or are being employed in the malaria vaccine development, focusing on the pharmaceutical and immunological aspects, as well as on their immunization outcomings at clinical and preclinical stages.This project was partially supported by the "Ministerio de Ciencia e Innovacion" (SAF2007-66115), the University of the Basque Country (UPV/EHU) (UFI 11/32), and FEDER funds. E. Mata thanks the Basque Government for a fellowship grant
Computational and Experimental Evaluation of the Immune Response of Neoantigens for Personalized Vaccine Design
Get PDFIn the last few years, the importance of neoantigens in the development of personalized antitumor vaccines has increased remarkably. In order to study whether bioinformatic tools are effective in detecting neoantigens that generate an immune response, DNA samples from patients with cutaneous melanoma in different stages were obtained, resulting in a total of 6048 potential neoantigens gathered. Thereafter, the immunological responses generated by some of those neoantigens ex vivo were tested, using a vaccine designed by a new optimization approach and encapsulated in nanoparticles. Our bioinformatic analysis indicated that no differences were found between the number of neoantigens and that of non-mutated sequences detected as potential binders by IEDB tools. However, those tools were able to highlight neoantigens over non-mutated peptides in HLA-II recognition (p-value 0.03). However, neither HLA-I binding affinity (p-value 0.08) nor Class I immunogenicity values (p-value 0.96) indicated significant differences for the latter parameters. Subsequently, the new vaccine, using aggregative functions and combinatorial optimization, was designed. The six best neoantigens were selected and formulated into two nanoparticles, with which the immune response ex vivo was evaluated, demonstrating a specific activation of the immune response. This study reinforces the use of bioinformatic tools in vaccine development, as their usefulness is proven both in silico and ex vivo.This work was supported by Basque Government funding (IT456-22; IT1448-22, IT693-22 and IT1524-22; ONKOVAC 2021111042), as well as by the UPV/EHU (GIU20/035; US21/27; US18/21; PIF18/295) and Basque Center of Applied Mathematics (US21/27 and US18/21)
Dermofarmazia. Osasuna kanpoaldetik zaintzea
No full text196 p.Aurkibidea:
1. Sarrera.
2. Larruazalaren eta larruazal-eranskinen histologia eta fisiologÃa.
3. Aurpegiaren garbiketa.
4. Aurpegiaren hidratazioa.
5. Aknea.
6. Larruazala zahartzea.
7. Larruazalaren pigmentazioa.
8. Eguzki-babesa.
9. Zelulitisa eta ildoak.
10. Bainurako prestakinak.
11. Ilearen garbiketa.
12. Ilea zaintzeko beste produktu batzuk.
13. Buruko ile-larruaren kosmetika. Ileko arazoak.
14. Zahia eta seborrea.
15. Ahoko higienea.
16. Bizarrerako produktuak.
17. Produktu kosmetikoen kontrolak eta legeria
Overcoming the Inflammatory Stage of Non-Healing Wounds: In Vitro Mechanism of Action of Negatively Charged Microspheres (NCMs)
Get PDFNegatively charged microspheres (NCMs) represent a new therapeutic approach for wound healing since recent clinical trials have shown NCM efficacy in the recovery of hard-to-heal wounds that tend to stay in the inflammatory phase, unlocking the healing process. The aim of this study was to elucidate the NCM mechanism of action. NCMs were extracted from a commercial microsphere formulation (PolyHeal® Micro) and cytotoxicity, attachment, proliferation and viability assays were performed in keratinocytes and dermal fibroblasts, while macrophages were used for the phagocytosis and polarization assays. We demonstrated that cells tend to attach to the microsphere surface, and that NCMs are biocompatible and promote cell proliferation at specific concentrations (50 and 10 NCM/cell) by a minimum of 3 fold compared to the control group. Furthermore, NCM internalization by macrophages seemed to drive these cells to a noninflammatory condition, as demonstrated by the over-expression of CD206 and the under-expression of CD64, M2 and M1 markers, respectively. NCMs are an effective approach for reverting the chronic inflammatory state of stagnant wounds (such as diabetic wounds) and thus for improving wound healing.The present work was partially funded by the Basque Government (Consolidated Groups, IT-907-16 and HAZITEK, ZE-2017/00014) and co-funded by the European Regional Development Fund (ERDF)
