The effect of intestinal ischemia on plasma thiol/disulphide homeostasis in an experimental study

Aim: To investigate the effects of acute intestinal ischemia on plasma thiol/disulphide homeostasis (TDSH), which has been investigated in a limited number of studies in the related literature. Methods: Twenty-four rats were randomized into control (operation without ischemia, GIS), and ischemia groups (GII-60, GIII-180). For ischemia, the superior mesenteric artery was sutured and the rats were exposed to 60 and 180 minutes of intestinal ischemia, respectively. Plasma TDSH was measured in blood samples collected at the end of the ischemia, and the pathology of ileum segments resected was evaluated. Results: The experimental ischemic conditions provided were confirmed by the total histopathological scoring system statistically. The levels of serum human albumin and ischemia modified albumin (IMA) in groups were detected in quite a close range of each other. There was no found a statistically significant difference for IMA between groups (p>0.05).  The alternations on the levels of plasma TDSH parameters were observed in the study. According to ischemic conditions, the thiol/disulfide ratio fluctuations were detected in the plasma TDSH. The native thiol and total thiol levels seem to have decreased according to ischemia; no statistical difference was detected. In addition, the disulfide levels increasing according to ischemia either was not found significant statistically (p>0.05). Conclusion: Although this study showed the oxidative balance in intestinal ischemia had affected plasma TDSH, also it revealed that intestinal ischemia didn't create a statistically significant difference between plasma TDSH components

The response of total testing process in clinical laboratory medicine to COVID-19 pandemic

Following a pandemic, laboratory medicine is vulnerable to laboratory errors due to the stressful and high workloads. We aimed to examine how laboratory errors may arise from factors, e.g., flexible working order, staff displacement, changes in the number of tests, and samples will reflect on the total test process (TTP) during the pandemic period. In 12 months, 6 months before and during the pandemic, laboratory errors were assessed via quality indicators (QIs) related to TTP phases. QIs were grouped as pre-, intra- and postanalytical. The results of QIs were expressed in defect percentages and sigma, evaluated with 3 levels of performance quality: 25th, 50th and 75th percentile values. When the pre- and during pandemic periods were compared, the sigma value of the samples not received was significantly lower in pre-pandemic group than during pandemic group (4.7σ vs. 5.4σ, P = 0.003). The sigma values of samples transported inappropriately and haemolysed samples were significantly higher in pre-pandemic period than during pandemic (5.0σ vs. 4.9σ, 4.3σ vs. 4.1σ; P = 0.046 and P = 0.044, respectively). Sigma value of tests with inappropriate IQC performances was lower during pandemic compared to the pre-pandemic period (3.3σ vs. 3.2σ, P = 0.081). Sigma value of the reports delivered outside the specified time was higher during pandemic than pre-pandemic period (3.0σ vs. 3.1σ, P = 0.030). In all TTP phases, some quality indicators improved while others regressed during the pandemic period. It was observed that preanalytical phase was affected more by the pandemic

The Role of the Lipid Profile and Oxidative Stress in Fatigue, Sleep Disorders and Cognitive Impairment in Patients with Multiple Sclerosis

The aim of this study is to investigate the relationship of the lipid profile, dysfunctional high-density lipoprotein, ischaemia-modified albumin and thiol–disulfide homeostasis with cognitive impairment, fatigue and sleep disorders in patients with multiple sclerosis. The cognitive functions of patients were evaluated with the Brief International Cognitive Assessment for Multiple Sclerosis battery. Fatigue was evaluated with the Fatigue Severity Scale and the Fatigue Impact Scale. The Pittsburgh Sleep Quality Index and the Epworth Sleepiness Scale were used to assess patients’ sleep disturbance. Peripheral blood samples were collected, and lipid levels and myeloperoxidase and paraoxonase activity were measured. The myeloperoxidase/paraoxonase ratio, which indicates dysfunctional high-density lipoprotein, was calculated. Thiol–disulfide homeostasis and ischaemia-modified albumin were measured. We did not identify any relationship between dysfunctional high-density lipoprotein and the physical disability, cognitive decline, fatigue and sleep problems of multiple sclerosis. Thiol–disulfide homeostasis was associated with cognitive scores. The shift of the balance towards disulfide was accompanied by a decrease in cognitive scores. On the other hand, we did not detect any relationship between fatigue and sleep disorders and thiol–disulfide homeostasis. Our findings revealed a possible correlation between cognitive dysfunction and thiol–disulfide homeostasis in multiple sclerosis patients

The relationship between thiol-disulfide balance and prostate cancer

This study aimed to investigatethe possible association of thiol/disulfidehomeostasiswith prostate-specific antigen levelsin prostate cancer patients and to compare the results with a normal healthy population ofa similar age group for the first time in literature. Forty-twopatients (20 patientswith prostate cancer and 22 volunteers) were included in the study. Thiol/disulfide homeostasis tests were measured with an automated method. Albumin, carcinoembryonic antigen (CEA), prostate-specific antigen (PSA), ischemia modified albumin (IMA), total thiol (TT), native thiol (SH), and disulfide (SS) levelsand, thiol-disulfide ratios (disulfide / native thiol, disulfide/total thiol and native thiol/total thiol) were assessed to detect any differences between prostate cancer group and control group. The mean PSA value of the prostate cancer group was 2.56 ng/mL, the mean PSA value of the control group was 1.21 ng/mL, and the mean age of the prostate cancer group was 67.32 years, the mean age of control group was 60.09. Although native thiol and total thiol levels were significantly lower in patients with prostate cancer (p 0.05). In our study, we could not show thiol-disulfide values as a biochemical prognostic factor in patients with prostate cancer. We believe that serum TT, SH, SS concentrations cannot serve as a noninvasive biomarker for prostate cancer. To verify the biochemical role of thiol/disulfide balance, studies need to be done by increasing the number of patients with prostate cancer. [Med-Science 2020; 9(3.000): 779-83

Evaluation of periodontal status in different phenotypes of polycystic ovary syndrome in untreated patients of early reproductive age: A case-control study

GURLEK, BERIL/0000-0002-4050-3193;WOS: 000506464700001PubMed: 31922343Aim A few studies demonstrated an increased risk of periodontal disease in patients with polycystic ovary syndrome (PCOS). the aim of this study was to evaluate the periodontal status in women of reproductive age who were recently diagnosed with different phenotypes of PCOS. Methods This prospectively designed cross-sectional case-control study included 116 consecutive subjects with PCOS and 90 healthy volunteers, aged between 18 and 24, who were admitted to the gynecology polyclinic between April 2018 and December 2018. All subjects of PCOS diagnosed using the Rotterdam criteria were subdivided into four phenotypes based on clinical, biochemical and ultrasonographic parameters according to the guidelines of the National Institutes of Health. the same dentist investigated periodontal parameters including plaque index, gingival index, bleeding on probing (%), probing depth, clinical attachment loss and the decayed, missing, filled teeth index. Results Probing depth, a periodontal parameter, was higher in all sub-phenotypes of the PCOS group compared to the control group. There was no difference in other periodontal parameters. Conclusion Periodontal probing depth, which is an important parameter in the diagnosis of periodontal diseases, was higher in all phenotypic subgroups of PCOS in early reproductive age

Plasma thiol levels are associated with disease severity in nonsegmental vitiligo

Background: Vitiligo is a depigmenting cutaneous disorder with complex pathogenesis. Thiol compounds are well-known organic structures that play a major role in melanogenesis. Aim: The aim of this study was to determine the association between plasma thiol level and disease severity in patients with nonsegmental vitiligo. Methods: A total of 73 patients with nonsegmental vitiligo (57 generalized and 16 localized type) and age- and sex-matched 69 healthy controls were enrolled in the study. Plasma levels of native thiols, disulfides, and total thiols were measured by a novel and automated assay. Disease severity of vitiligo was assessed with Vitiligo Area Scoring Index (VASI) score. The extent, stage, and spread of vitiligo of patients were evaluated according to the Vitiligo European Task Force (VETF) system. Results: The native and total thiol levels of vitiligo patients were higher than those of healthy control group (P≤0.001 and 0.001, respectively). The median VASI score of patients was 0.7 (0.02–28.30). Univariate analyses showed that plasma native thiol levels, VETF spread score, disease duration, and vitiligo type significantly correlated with VASI scores (r=0.237, P=0.043; r=0.458, P<0.001; and P<0.001, respectively). Stepwise multivariate analysis revealed that disease duration (β=0.017; P=0.005) and spread score (β=1.301; P=0.001) were found statistically significant as independent factors on VASI score. Conclusion: Although plasma native thiol level significantly correlated with VASI scores of patients, it is not a predictive factor for vitiligo severity

Total antioxidant capacity and total oxidant status of synovial fluids in patients with temporomandibular joint pain and dysfunction

Objectives The objective of this study was to investigate whether a relationship exists between total antioxidant capacity (TAC) and total oxidant status (TOS) of synovial fluids (SFs) of temporomandibular joint (TMJ) pain patients with pain and dysfunction

Total antioxidant capacity and total oxidant status of synovial fluids in patients with temporomandibular joint pain and dysfunction

Objectives The objective of this study was to investigate whether a relationship exists between total antioxidant capacity (TAC) and total oxidant status (TOS) of synovial fluids (SFs) of temporomandibular joint (TMJ) pain patients with pain and dysfunction

The Role of Ischemia-modified Albumin and Ischemia-Modified Albumin to Albumin Ratios in Patients with Alopecia Areata

Introduction: Objective: To investigate the role of ischemia-modified albumin (IMA) and IMA/albumin levels in patients with AA. Methods: The present prospective crossectional study includes patients & GE;18 who were admitted to the Dermatology and Venerology Department of Hitit University Hospital between April 1, 2021, and September 30, 2021. 70 patients participated in the study (n=34 for the study group and n=36 for the control group). Demographic features, clinical characteristics, IMA, and IMA/albumin levels were compared between the groups. The study group was divided into subgroups based on the number of patches, disease duration, and the number of disease attacks. IMA and IMA/albumin levels were compared between each subgroup. Results: The study and control groups were similar with regard to demographic features and clinical characteristics. Significant differences were observed between the mean IMA and IMA/albumin ratio (p=0.004 and 0.012, respectively). The study subgroups were comparable in the number of patches, disease duration, and number of disease attacks. Conclusion: Although oxidative stress is an important component in the etiology of AA, IMA and IMA/albumin may not be useful in the prediction of disease severity in patients with AA

Does Subclinical Hypothyroidism Affect Dynamic Thiol/Disulfide Homeostasis and ischemia-modified Albumin Levels in Children?

karahan, irfan/0000-0003-4669-1751WOS:000561256500010PubMed: 32811603Objective: To determine the effects of subclinical hypothyroidism on oxidative stress in children. Study Design: A cross-sectional study. Place and Duration of Study: Department of Paediatrics, Paediatric Endocrinology, and General Outpatient Clinics, Kirikkale University, School of Medicine, from May 2017 to October 2018. Methodology: This study included 92 subjects aged between 2 and 18 years. The subjects were divided into two groups. Forty-seven children with subclinical hypothyroidism and 45 healthy controls were evaluated. In order to evaluate oxidative damage, native thiol, total thiol, disulfides, their ratios, and ischemia-modified albumin (IMA) levels were compared between the two groups. The relationship between TSH and IMA levels was assessed. Results: Age and gender were not significantly different in the two groups. Native thiol, total thiol, disulfides and their ratios were similar in the two groups. lschemia-modified albumin levels were significantly higher in the patient group than the controls (p<0.001). There was no correlation between TSH and IMA levels in the patient group (r=0.069 p=0.645). Conclusion: Subclinical hypothyroidism may be related to the impairment of IMA, and have a neutral effect on thiol/disulfide balance. Further research is needed to explain the effects of oxidative stress in subclinical hypothyroidism