The effect of intestinal ischemia on plasma thiol/disulphide homeostasis in an experimental study

Aim: To investigate the effects of acute intestinal ischemia on plasma thiol/disulphide homeostasis (TDSH), which has been investigated in a limited number of studies in the related literature. Methods: Twenty-four rats were randomized into control (operation without ischemia, GIS), and ischemia groups (GII-60, GIII-180). For ischemia, the superior mesenteric artery was sutured and the rats were exposed to 60 and 180 minutes of intestinal ischemia, respectively. Plasma TDSH was measured in blood samples collected at the end of the ischemia, and the pathology of ileum segments resected was evaluated. Results: The experimental ischemic conditions provided were confirmed by the total histopathological scoring system statistically. The levels of serum human albumin and ischemia modified albumin (IMA) in groups were detected in quite a close range of each other. There was no found a statistically significant difference for IMA between groups (p>0.05).  The alternations on the levels of plasma TDSH parameters were observed in the study. According to ischemic conditions, the thiol/disulfide ratio fluctuations were detected in the plasma TDSH. The native thiol and total thiol levels seem to have decreased according to ischemia; no statistical difference was detected. In addition, the disulfide levels increasing according to ischemia either was not found significant statistically (p>0.05). Conclusion: Although this study showed the oxidative balance in intestinal ischemia had affected plasma TDSH, also it revealed that intestinal ischemia didn't create a statistically significant difference between plasma TDSH components

The response of total testing process in clinical laboratory medicine to COVID-19 pandemic

Following a pandemic, laboratory medicine is vulnerable to laboratory errors due to the stressful and high workloads. We aimed to examine how laboratory errors may arise from factors, e.g., flexible working order, staff displacement, changes in the number of tests, and samples will reflect on the total test process (TTP) during the pandemic period. In 12 months, 6 months before and during the pandemic, laboratory errors were assessed via quality indicators (QIs) related to TTP phases. QIs were grouped as pre-, intra- and postanalytical. The results of QIs were expressed in defect percentages and sigma, evaluated with 3 levels of performance quality: 25th, 50th and 75th percentile values. When the pre- and during pandemic periods were compared, the sigma value of the samples not received was significantly lower in pre-pandemic group than during pandemic group (4.7σ vs. 5.4σ, P = 0.003). The sigma values of samples transported inappropriately and haemolysed samples were significantly higher in pre-pandemic period than during pandemic (5.0σ vs. 4.9σ, 4.3σ vs. 4.1σ; P = 0.046 and P = 0.044, respectively). Sigma value of tests with inappropriate IQC performances was lower during pandemic compared to the pre-pandemic period (3.3σ vs. 3.2σ, P = 0.081). Sigma value of the reports delivered outside the specified time was higher during pandemic than pre-pandemic period (3.0σ vs. 3.1σ, P = 0.030). In all TTP phases, some quality indicators improved while others regressed during the pandemic period. It was observed that preanalytical phase was affected more by the pandemic

The relationship between thiol-disulfide balance and prostate cancer

This study aimed to investigatethe possible association of thiol/disulfidehomeostasiswith prostate-specific antigen levelsin prostate cancer patients and to compare the results with a normal healthy population ofa similar age group for the first time in literature. Forty-twopatients (20 patientswith prostate cancer and 22 volunteers) were included in the study. Thiol/disulfide homeostasis tests were measured with an automated method. Albumin, carcinoembryonic antigen (CEA), prostate-specific antigen (PSA), ischemia modified albumin (IMA), total thiol (TT), native thiol (SH), and disulfide (SS) levelsand, thiol-disulfide ratios (disulfide / native thiol, disulfide/total thiol and native thiol/total thiol) were assessed to detect any differences between prostate cancer group and control group. The mean PSA value of the prostate cancer group was 2.56 ng/mL, the mean PSA value of the control group was 1.21 ng/mL, and the mean age of the prostate cancer group was 67.32 years, the mean age of control group was 60.09. Although native thiol and total thiol levels were significantly lower in patients with prostate cancer (p 0.05). In our study, we could not show thiol-disulfide values as a biochemical prognostic factor in patients with prostate cancer. We believe that serum TT, SH, SS concentrations cannot serve as a noninvasive biomarker for prostate cancer. To verify the biochemical role of thiol/disulfide balance, studies need to be done by increasing the number of patients with prostate cancer. [Med-Science 2020; 9(3.000): 779-83

Total antioxidant capacity and total oxidant status of synovial fluids in patients with temporomandibular joint pain and dysfunction

Objectives The objective of this study was to investigate whether a relationship exists between total antioxidant capacity (TAC) and total oxidant status (TOS) of synovial fluids (SFs) of temporomandibular joint (TMJ) pain patients with pain and dysfunction

Total antioxidant capacity and total oxidant status of synovial fluids in patients with temporomandibular joint pain and dysfunction

Objectives The objective of this study was to investigate whether a relationship exists between total antioxidant capacity (TAC) and total oxidant status (TOS) of synovial fluids (SFs) of temporomandibular joint (TMJ) pain patients with pain and dysfunction

Does Subclinical Hypothyroidism Affect Dynamic Thiol/Disulfide Homeostasis and ischemia-modified Albumin Levels in Children?

karahan, irfan/0000-0003-4669-1751WOS:000561256500010PubMed: 32811603Objective: To determine the effects of subclinical hypothyroidism on oxidative stress in children. Study Design: A cross-sectional study. Place and Duration of Study: Department of Paediatrics, Paediatric Endocrinology, and General Outpatient Clinics, Kirikkale University, School of Medicine, from May 2017 to October 2018. Methodology: This study included 92 subjects aged between 2 and 18 years. The subjects were divided into two groups. Forty-seven children with subclinical hypothyroidism and 45 healthy controls were evaluated. In order to evaluate oxidative damage, native thiol, total thiol, disulfides, their ratios, and ischemia-modified albumin (IMA) levels were compared between the two groups. The relationship between TSH and IMA levels was assessed. Results: Age and gender were not significantly different in the two groups. Native thiol, total thiol, disulfides and their ratios were similar in the two groups. lschemia-modified albumin levels were significantly higher in the patient group than the controls (p<0.001). There was no correlation between TSH and IMA levels in the patient group (r=0.069 p=0.645). Conclusion: Subclinical hypothyroidism may be related to the impairment of IMA, and have a neutral effect on thiol/disulfide balance. Further research is needed to explain the effects of oxidative stress in subclinical hypothyroidism

The utility of ischemia modified albumin as an oxidative stress biomarker in seborrheic dermatitis

Aim: Seborrheic dermatitis (SD) is a commonly seen chronic inflammatory skin disease that occurs as scaly reddish-brown itchy patches on sebaceous, gland-rich areas of the scalp, face, and trunk. The relation between SD disease and serum IMA (Ischemia modified albumin) levels remains unknown. To investigate the potential role of serum IMA and corrected IMA levels in SD disease. Materials and Methods: Thirty-seven participants who were diagnosed with SD disease and sixty-two healthy subjects (control group) were enrolled in the study. Venous blood samples were collected from each participant, and serum IMA was measured spectrophotometrically using the albumin cobalt binding test. Results: The serum IMA and corrected IMA levels were statistically significant between the groups, and the levels of IMA and corrected IMA were measured as SD patients group 0.70, 0.70 and control group 0.52, 0.51 ABSU (absorbance units), respectively (p < 0.05). Moreover, serum IMA and corrected IMA levels were statistically significant between male and female groups in terms of gender as 0.75 and 0.69 ABSU, respectively (p < 0.05). Serum albumin levels, age, and BMI (body mass index) were statistically insignificant between these groups. Conclusion: Our results show increased serum IMA and corrected IMA levels in patients with SD. Evaluation of IMA and corrected IMA levels in SD disease may contribute to diagnosis and prognosis. Further and comprehensive studies are neede