15 research outputs found
Impaired Ciliogenesis in differentiating human bronchial epithelia exposed to non-Cytotoxic doses of multi-walled carbon Nanotubes
Abstract Background Multi-walled carbon nanotubes (MWCNTs) are engineered nanomaterials used for a variety of industrial and consumer products. Their high tensile strength, hydrophobicity, and semi-conductive properties have enabled many novel applications, increasing the possibility of accidental nanotube inhalation by either consumers or factory workers. While MWCNT inhalation has been previously shown to cause inflammation and pulmonary fibrosis at high doses, the susceptibility of differentiating bronchial epithelia to MWCNT exposure remains unexplored. In this study, we investigate the effect of MWCNT exposure on cilia development in a differentiating air-liquid interface (ALI) model. Primary bronchial epithelial cells (BECs) were isolated from human donors via bronchoscopy and treated with non-cytotoxic doses of MWCNTs in submerged culture for 24 h. Cultures were then allowed to differentiate in ALI for 28 days in the absence of further MWCNT exposure. At 28 days, mucociliary differentiation endpoints were assessed, including whole-mount immunofluorescent staining, histological, immunohistochemical and ultrastructural analysis, gene expression, and cilia beating analysis. Results We found a reduction in the prevalence and beating of ciliated cells in MWCNT-treated cultures, which appeared to be caused by a disruption of cellular microtubules and cytoskeleton during ciliogenesis and basal body docking. Expression of gene markers of mucociliary differentiation, such as FOXJ1 and MUC5AC/B, were not affected by treatment. Colocalization of basal body marker CEP164 with γ-tubulin during days 1–3 of ciliogenesis, as well as abundance of basal bodies up to day 14, were attenuated by treatment with MWCNTs. Conclusions Our results suggest that a single exposure of bronchial cells to MWCNT during a vulnerable period before differentiation may impair their ability to develop into fully functional ciliated cells
The TESS Triple-9 Catalog II: a new set of 999 uniformly-vetted exoplanet candidates
The Transiting Exoplanet Survey Satellite (TESS) mission is providing the
scientific community with millions of light curves of stars spread across the
whole sky. Since 2018 the telescope has detected thousands of planet candidates
that need to be meticulously scrutinized before being considered amenable
targets for follow-up programs. We present the second catalog of the Plant
Patrol citizen science project containing 999 uniformly-vetted exoplanet
candidates within the TESS ExoFOP archive. The catalog was produced by fully
exploiting the power of the Citizen Science Planet Patrol project. We vetted
TESS Objects of Interest (TOIs) based on the results of Discovery And Vetting
of Exoplanets DAVE pipeline. We also implemented the Automatic Disposition
Generator, a custom procedure aimed at generating the final classification for
each TOI that was vetted by at least three vetters. The majority of the
candidates in our catalog, TOIs, passed the vetting process and were
labelled as planet candidates. We ruled out candidates as false positives
and flagged as potential false positives. Our final dispositions and
comments for all the planet candidates are provided as a publicly available
supplementary table.Comment: 17 pages, 11 figures, 5 tables. Accepted for publication on MNRA
Relevansi Pemikiran Pendidikan KH. Imam Zarkasyi Dalam Pembentukan Intrapersonal Intelegensi
This article discusses about the relevance of KH Imam Zarkasi thoughts of education in developing intrapersonal intelligence. It is a kind of library research using descriptive-qualitative method. The resutt of this research shows that KH. Imam Zarkasi`s thoughts are still very relevant to be implemented in developing students` intrapersonal intelligence. Among of his thoughts are; 1) developing intrapersonal intelligence can be through two ways; intentionally, that is through education in formal institutions, and unintentionally that is through interaction with the surrounding community and the nature. 2) The method of learning to develop intrapersonal intelligence is through individual training; by entrusting children to carry out their own duties with full responsibility, giving children the opportunity to make their own decisions and be responsible for their decisions, and other similar activities. These thoughts have been applied by KH Imam Zarkasi through the institution he developed, namely Pondok Pesantren Gontor. Among of his renewal efforts are; The implementation learning using classical system, the use of learning methods that provide opportunities for children to develop their thinking power critically, independently and responsibly, and provide extracurricular activities as a forum for students to develop themselves.
Education Thoughts, KH. Imam Zarkasyi, Intrapersonal Intelligenc
La Charente
16 janvier 19101910/01/16 (A39,N14968)-1910/01/16.Appartient à l’ensemble documentaire : PoitouCh
The TESS Triple-9 Catalog: 999 uniformly vetted candidate exoplanets
The Transiting Exoplanet Survey Satellite (TESS) has detected thousands of
exoplanet candidates since 2018, most of which have yet to be confirmed. A key
step in the confirmation process of these candidates is ruling out false
positives through vetting. Vetting also eases the burden on follow-up
observations, provides input for demographics studies, and facilitates training
machine learning algorithms. Here we present the TESS Triple-9 (TT9) catalog --
a uniformly-vetted catalog containing dispositions for 999 exoplanet candidates
listed on ExoFOP-TESS, known as TESS Objects of Interest (TOIs). The TT9 was
produced using the Discovery And Vetting of Exoplanets pipeline, DAVE, and
utilizing the power of citizen science as part of the Planet Patrol project.
More than 70% of the TOIs listed in the TT9 pass our diagnostic tests, and are
thus marked as true planetary candidates. We flagged 144 candidates as false
positives, and identified 146 as potential false positives. At the time of
writing, the TT9 catalog contains ~20% of the entire ExoFOP-TESS TOIs list,
demonstrates the synergy between automated tools and citizen science, and
represents the first stage of our efforts to vet all TOIs. The DAVE generated
results are publicly available on ExoFOP-TESS.Comment: 15 pages, 11 figures. Accepted on MNRA