Impaired Ciliogenesis in differentiating human bronchial epithelia exposed to non-Cytotoxic doses of multi-walled carbon Nanotubes

Abstract Background Multi-walled carbon nanotubes (MWCNTs) are engineered nanomaterials used for a variety of industrial and consumer products. Their high tensile strength, hydrophobicity, and semi-conductive properties have enabled many novel applications, increasing the possibility of accidental nanotube inhalation by either consumers or factory workers. While MWCNT inhalation has been previously shown to cause inflammation and pulmonary fibrosis at high doses, the susceptibility of differentiating bronchial epithelia to MWCNT exposure remains unexplored. In this study, we investigate the effect of MWCNT exposure on cilia development in a differentiating air-liquid interface (ALI) model. Primary bronchial epithelial cells (BECs) were isolated from human donors via bronchoscopy and treated with non-cytotoxic doses of MWCNTs in submerged culture for 24 h. Cultures were then allowed to differentiate in ALI for 28 days in the absence of further MWCNT exposure. At 28 days, mucociliary differentiation endpoints were assessed, including whole-mount immunofluorescent staining, histological, immunohistochemical and ultrastructural analysis, gene expression, and cilia beating analysis. Results We found a reduction in the prevalence and beating of ciliated cells in MWCNT-treated cultures, which appeared to be caused by a disruption of cellular microtubules and cytoskeleton during ciliogenesis and basal body docking. Expression of gene markers of mucociliary differentiation, such as FOXJ1 and MUC5AC/B, were not affected by treatment. Colocalization of basal body marker CEP164 with γ-tubulin during days 1–3 of ciliogenesis, as well as abundance of basal bodies up to day 14, were attenuated by treatment with MWCNTs. Conclusions Our results suggest that a single exposure of bronchial cells to MWCNT during a vulnerable period before differentiation may impair their ability to develop into fully functional ciliated cells

The TESS Triple-9 Catalog II: a new set of 999 uniformly-vetted exoplanet candidates

The Transiting Exoplanet Survey Satellite (TESS) mission is providing the scientific community with millions of light curves of stars spread across the whole sky. Since 2018 the telescope has detected thousands of planet candidates that need to be meticulously scrutinized before being considered amenable targets for follow-up programs. We present the second catalog of the Plant Patrol citizen science project containing 999 uniformly-vetted exoplanet candidates within the TESS ExoFOP archive. The catalog was produced by fully exploiting the power of the Citizen Science Planet Patrol project. We vetted TESS Objects of Interest (TOIs) based on the results of Discovery And Vetting of Exoplanets DAVE pipeline. We also implemented the Automatic Disposition Generator, a custom procedure aimed at generating the final classification for each TOI that was vetted by at least three vetters. The majority of the candidates in our catalog, $752$ TOIs, passed the vetting process and were labelled as planet candidates. We ruled out $142$ candidates as false positives and flagged $105$ as potential false positives. Our final dispositions and comments for all the planet candidates are provided as a publicly available supplementary table.Comment: 17 pages, 11 figures, 5 tables. Accepted for publication on MNRA

Relevansi Pemikiran Pendidikan KH. Imam Zarkasyi Dalam Pembentukan Intrapersonal Intelegensi

This article discusses about the relevance of KH Imam Zarkasi thoughts of education in developing intrapersonal intelligence. It is a kind of library research using descriptive-qualitative method. The resutt of this research shows that KH. Imam Zarkasi`s thoughts are still very relevant to be implemented in developing students` intrapersonal intelligence. Among of his thoughts are; 1) developing intrapersonal intelligence can be through two ways; intentionally, that is through education in formal institutions, and unintentionally that is through interaction with the surrounding community and the nature. 2) The method of learning to develop intrapersonal intelligence is through individual training; by entrusting children to carry out their own duties with full responsibility, giving children the opportunity to make their own decisions and be responsible for their decisions, and other similar activities. These thoughts have been applied by KH Imam Zarkasi through the institution he developed, namely Pondok Pesantren Gontor. Among of his renewal efforts are; The implementation learning using classical system, the use of learning methods that provide opportunities for children to develop their thinking power critically, independently and responsibly, and provide extracurricular activities as a forum for students to develop themselves. Education Thoughts, KH. Imam Zarkasyi, Intrapersonal Intelligenc

La Charente

16 janvier 19101910/01/16 (A39,N14968)-1910/01/16.Appartient à l’ensemble documentaire : PoitouCh

The TESS Triple-9 Catalog: 999 uniformly vetted candidate exoplanets

The Transiting Exoplanet Survey Satellite (TESS) has detected thousands of exoplanet candidates since 2018, most of which have yet to be confirmed. A key step in the confirmation process of these candidates is ruling out false positives through vetting. Vetting also eases the burden on follow-up observations, provides input for demographics studies, and facilitates training machine learning algorithms. Here we present the TESS Triple-9 (TT9) catalog -- a uniformly-vetted catalog containing dispositions for 999 exoplanet candidates listed on ExoFOP-TESS, known as TESS Objects of Interest (TOIs). The TT9 was produced using the Discovery And Vetting of Exoplanets pipeline, DAVE, and utilizing the power of citizen science as part of the Planet Patrol project. More than 70% of the TOIs listed in the TT9 pass our diagnostic tests, and are thus marked as true planetary candidates. We flagged 144 candidates as false positives, and identified 146 as potential false positives. At the time of writing, the TT9 catalog contains ~20% of the entire ExoFOP-TESS TOIs list, demonstrates the synergy between automated tools and citizen science, and represents the first stage of our efforts to vet all TOIs. The DAVE generated results are publicly available on ExoFOP-TESS.Comment: 15 pages, 11 figures. Accepted on MNRA

Students' participation in collaborative research should be recognised

Letter to the editor