A educação sexual da comunidade surda: perspectiva da enfermagem / The deaf community sex education: nursing perspective

O tema em questão constitui como objetivo debater as questões sobre sexualidade a partir de estudos que envolvem os surdos e as categorias de gênero, corpo, tradução cultural. Entende-se que a comunidade surda, não por opção, mas por condição, têm por “obrigação” aprender duas línguas: a que melhor responde às suas necessidades de comunicação, no caso a LIBRAS, e a língua padrão da sociedade em que estão inseridos. Dessa forma, além dos problemas provenientes da discriminação a que são submetidos, por serem vistos como “deficientes”, também precisam enfrentar as dificuldades próprias da falta de informação e comunicação em que se encontram, visto que o seu meio social é estruturado para pessoas ouvintes. As pessoas que possuem algum problema de audição são denominadas como “deficientes auditivos”, ou DA. Segundo Behares (1993 apud NÉLO 1995), “deficiente auditivo é um dos termos utilizados para designar as pessoas que apresentam algum grau de surdez, podendo ter quatro níveis: leve; média ou moderada; severa; e profunda”. Este termo parte de uma concepção médico-organicista que vê a pessoa surda como um ouvinte com defeito. No momento, existe uma discussão sobre o melhor termo de tratamento à estas pessoas. Nesse sentido, Nélo (1995) afirma que o termo “surdo” é mais apropriado para designar as pessoas surdas, por apresentar uma concepção mais particular sobre a surdez. Este termo parte de uma visão sócio-cultural que trata a surdez como um fenômeno com vários determinantes. A referida autora destaca que “o surdo não é diferente unicamente porque não ouve, mas sim porque desenvolve potencialidades psico-culturais diferentes das dos ouvintes” (NÉLO 1995 p.9). Podemos dizer então que as comunidades surdas desenvolvem comportamentos com características próprias de sua maneira específica de internalizar as coisas que acontecem ao seu redor, propiciando, assim, a formação de uma cultura surda. Considerando esta especificidade da cultura surda, o presente estudo tem por finalidade conhecer como são elaboradas as construções sociais de gênero e sexualidade no seio da mesma, procurando identificar as construções de masculinidades e feminilidades, a partir de aspectos relacionados à divisão sexual do trabalho, e conhecer os posicionamentos dos/as surdos/as acerca das vivências da sexualidade (homossexualidade, heterossexualidade, bissexualidade). Concebemos gênero como uma construção social que se expressa nas atitudes, nos valores, nas atividades públicas e privadas de homens e mulheres que fazem com que sejam vistos como tendo naturezas diferenciadas e, assim, posicionados socialmente como diferentes. Nesta perspectiva, o gênero, tomado enquanto categoria analítica, teoriza a questão da diferença sexual, indicando uma rejeição ao determinismo biológico implícito no uso dos termos como sexo ou diferença sexual

Phylogenetic relationships, distribution, and conservation of Roosmalens’ dwarf porcupine, Coendou roosmalenorum Voss & da Silva, 2001 (Rodentia, Erethizontidae)

The New World porcupines of the genus Coendou comprise 16 species of arboreal nocturnal rodents. Some of these species are poorly known and have not been included in phylogenetic analyses. Based on recently collected specimens with associated tissue from the Brazilian Amazonia, we investigate the distribution and phylogenetic relationships of Roosmalens’ dwarf porcupine, Coendou roosmalenorum, using an integrative approach using mitochondrial gene sequences and morphological data from new specimens and localities. Our results recovered C. roosmalenorum in the subgenus Caaporamys. However, analyses of our molecular and combined datasets produced different topologies. The new record shows the presence of C. roosmalenorum 480 km to the southeast of the Rio Madeira and 95 km away from Rio Juruena in Mato Grosso state, indicating a wider distribution in southern Amazonia than suspected. All known records of C. roosmalenorum are in the Madeira biogeographical province, to which it might be endemic

New karyotype for Mesomys stimulax (Rodentia, Echimyidae) from the Brazilian Amazon: A case for species complex?

Abstract Mesomys Wagner, 1845 (Rodentia, Echimyidae, Eumysopinae) currently has four recognized species, three of which occur in Brazil: Mesomys hispidus (probably a species complex), M. occultus, and M. stimulax. Mesomys leniceps is found in montane forests of northern Peru. Mesomys stimulax, the focus of the present study, has a distribution that is restricted to the central and eastern Amazonia south of the Amazon River, extending from the left bank of the Tapajós River to the right bank of the Tocantins River, and south to the southeast portion of Pará State. The genus presents karyotypes with diploid number 2n = 60 and Fundamental Number (FN) = 116 for M. hispidus and M. stimulax, and 2n = 42, FN = 54 for M. occultus. We studied the karyotype of a female specimen of M. stimulax collected from the Tapirapé‐Aquiri National Forest, Marabá, Pará, Brazil, in the Xingu/Tocantins interfluvium. The obtained karyotype (2n = 60 and FN = 110) differs from that described in the literature for both M. stimulax and M. hispidus by exhibiting more biarmed chromosomes, probably due to pericentric inversions and/or centromeric repositioning, and exhibiting differences in the amount and distribution of constitutive heterochromatin (CH). These results suggest that, similar to what has already been proposed for M. hispidus, M. stimulax may represent a species complex and/or cryptic species. The mechanisms of chromosomal diversification in Mesomys and the biogeographic implications are discussed reinforcing the need for broad systematic review for Mesomys

Phylogenetic relationships, distribution, and conservation of Roosmalens’ dwarf porcupine, Coendou roosmalenorum Voss & da Silva, 2001 (Rodentia, Erethizontidae)

The New World porcupines of the genus Coendou comprise 16 species of arboreal nocturnal rodents. Some of these species are poorly known and have not been included in phylogenetic analyses. Based on recently collected specimens with associated tissue from the Brazilian Amazonia, we investigate the distribution and phylogenetic relationships of Roosmalens’ dwarf porcupine, Coendou roosmalenorum, using an integrative approach using mitochondrial gene sequences and morphological data from new specimens and localities. Our results recovered C. roosmalenorum in the subgenus Caaporamys. However, analyses of our molecular and combined datasets produced different topologies. The new record shows the presence of C. roosmalenorum 480 km to the southeast of the Rio Madeira and 95 km away from Rio Juruena in Mato Grosso state, indicating a wider distribution in southern Amazonia than suspected. All known records of C. roosmalenorum are in the Madeira biogeographical province, to which it might be endemic

Supplementary material 3 from: Menezes FH, Semedo TBF, Saldanha J, Garbino GST, Fernandes-Ferreira H, Cordeiro-Estrela P, da Costa IR (2023) Phylogenetic relationships, distribution, and conservation of Roosmalens' dwarf porcupine, Coendou roosmalenorum Voss & da Silva, 2001 (Rodentia, Erethizontidae). ZooKeys 1179: 139-155. https://doi.org/10.3897/zookeys.1179.108766

Dataset

Supplementary material 4 from: Menezes FH, Semedo TBF, Saldanha J, Garbino GST, Fernandes-Ferreira H, Cordeiro-Estrela P, da Costa IR (2023) Phylogenetic relationships, distribution, and conservation of Roosmalens' dwarf porcupine, Coendou roosmalenorum Voss & da Silva, 2001 (Rodentia, Erethizontidae). ZooKeys 1179: 139-155. https://doi.org/10.3897/zookeys.1179.108766

Erethizontid cytochrome b sequences utilized in phylogenetic analyse

The emergence of a new sex-system (XX/XY1Y2) suggests a species complex in the "monotypic" rodent Oecomys auyantepui (Rodentia, Sigmodontinae).

X-autosome translocation (XY1Y2) has been reported in distinct groups of vertebrates suggesting that the rise of a multiple sex system within a species may act as a reproductive barrier and lead to speciation. The viability of this system has been linked with repetitive sequences located between sex and autosomal portions of the translocation. Herein, we investigate Oecomys auyantepui, using chromosome banding and Fluorescence In Situ Hybridization with telomeric and Hylaeamys megacephalus whole-chromosome probes, and phylogenetic reconstruction using mtDNA and nuDNA sequences. We describe an amended karyotype for O. auyantepui (2n = 64♀65♂/FNa = 84) and report for the first time a multiple sex system (XX/XY1Y2) in Oryzomyini rodents. Molecular data recovered O. auyantepui as a monophyletic taxon with high support and cytogenetic data indicate that O. auyantepui may exist in two lineages recognized by distinct sex systems. The Neo-X exhibits repetitive sequences located between sex and autosomal portions, which would act as a boundary between these two segments. The G-banding comparisons of the Neo-X chromosomes of other Sigmodontinae taxa revealed a similar banding pattern, suggesting that the autosomal segment in the Neo-X can be shared among the Sigmodontinae lineages with a XY1Y2 sex system

Detecção de potenciais marcadores moleculares séricos da doença de Hodgkin

MOTIVAÇÃO: Neste trabalho foi analisado o perfil de proteínas séricas de pacientes com doença de Hodgkin (DH) localizada e avançada em busca de novos e potenciais biomarcadores para o diagnóstico médico. MATERIAIS E MÉTODOS: O perfil de proteínas presentes no soro de 14 indivíduos saudáveis, 14 pacientes com DH avançada e 15 pacientes com DH localizada, assim como pools de soro dos respectivos grupos, foi analisado em gel desnaturante de poliacrilamida a 12% corado pela prata. A densitometria e a intensidade média das bandas de interesse foram estudadas utilizando-se o Kodak 1D Scientific Imaging System. RESULTADOS E CONCLUSÕES: O perfil protéico apresentou acentuada variação entre os pacientes examinados; entretanto foi observada a indução predominante de determinadas proteínas (aproximadamente 26kDa e 18kDa), cuja expressão foi substancialmente diferente quando em comparação com os controles (p < 0,01). Estas proteínas podem potencialmente constituir-se em marcadores moleculares de acompanhamento da evolução e do tratamento da doença

X‑rays Reveal the Internal Structure of Keratin Bundles in Whole Cells

In recent years, X-ray imaging of biological cells has emerged as a complementary alternative to fluorescence and electron microscopy. Different techniques were established and successfully applied to macromolecular assemblies and structures in cells. However, while the resolution is reaching the nanometer scale, the dose is increasing. It is essential to develop strategies to overcome or reduce radiation damage. Here we approach this intrinsic problem by combing two different X-ray techniques, namely ptychography and nanodiffraction, in one experiment and on the same sample. We acquire low dose ptychography overview images of whole cells at a resolution of 65 nm. We subsequently record high-resolution nanodiffraction data from regions of interest. By comparing images from the two modalities, we can exclude strong effects of radiation damage on the specimen. From the diffraction data we retrieve quantitative structural information from intracellular bundles of keratin intermediate filaments such as a filament radius of 5 nm, hexagonal geometric arrangement with an interfilament distance of 14 nm and bundle diameters on the order of 70 nm. Thus, we present an appealing combined approach to answer a broad range of questions in soft-matter physics, biophysics and biology

Genetic diversity analysis in the Brazilian Amazon reveals a new evolutionary lineage and new karyotype for the genus Mesomys (Rodentia, Echimyidae, Eumysopinae).

Morphological, molecular and chromosomal studies in the genera Lonchothrix and Mesomys have contributed to a better understanding of taxonomic design, phylogenetic relationships and karyotypic patterns. Recent molecular investigations have shown a yet undescribed diversity, suggesting that these taxa are even more diverse than previously assumed. Furthermore, some authors have questioned the limits of geographic distribution in the Amazon region for the species M. hispidus and M. stimulax. In this sense, the current study sought to understand the karyotypic evolution and geographic limits of the genus Mesomys, based on classical (G- and C-banding) and molecular cytogenetic analysis (FISH using rDNA 18S and telomeric probes) and through the sequencing of mitochondrial genes Cytochrome b (Cytb) and Cytochrome Oxidase-Subunit I (CO using phylogeny, species delimitation and time of divergence, from samples of different locations in the Brazilian Amazon. The species M. stimulax and Mesomys sp. presented 2n = 60/FN = 110, while M. hispidus presented 2n = 60/FN = 112, hitherto unpublished. Molecular dating showed that Mesomys diversification occurred during the Plio-Pleistocene period, with M. occultus diverging at around 5.1 Ma, followed by Mesomys sp. (4.1 Ma) and, more recently, the separation between M. hispidus and M. stimulax (3.5 Ma). The ABGD and ASAP species delimiters support the formation of 7 and 8 potential species of the genus Mesomys, respectively. Furthermore, in both analyzes Mesomys sp. was recovered as a valid species. Our multidisciplinary approach involving karyotypic, molecular and biogeographic analysis is the first performed in Mesomys, with the description of a new karyotype for M. hispidus, a new independent lineage for the genus and new distribution data for M. hispidus and M. stimulax