Production d’alevins : 2.5 Action n°19

National audienceDans ce chapitre, seules les principales méthodologies employées durant la saison 2008 ont été décrites. Elles devront évoluer dans les années à venir en fonction de l’avancée des connaissances et des retours d’expériences

Réalisation de reproductions assistées en 2009 : 2.2 Action n°15

Extrait de documentLes indicateurs utilisés classiquement pour le suivi de la maturation des esturgeons sont décrits. Ils manquent de précision et permettent principalement de comparer l’état de maturation des femelles entre elles. L’intérêt de nouveaux indicateurs: physiologiques (dosage d'analytes sanguins) et morphologique (TCJ) est discuté à partir de premières données obtenues chez A.sturio

Réalisation de reproductions assistées en 2008 : 2.3 Action n°15

Extrait de documentRésultats des biopsies, cultures in vitro. Reproduction du 29 mai 2009

Recent progress in larval rearing of the European sturgeon, Acipenser sturio: chap. 33

International audienceThis chapter deals with updated larvae management techniques, focusing on feed management. A compound diet was produced, and the reasoning behind this, the choice of ingredients, and manufacturing process are described here. Stocking, feeding sequences and results are also given. Co-feeding was based successively on 1-day-old Artemia salina nauplii (7–35 dph), chironomids (20–49 dph), and the compound diets (from 29 dph onwards). For 65-day-old fish raised at a water temperature range of 18.5–19C, high survival rates (89%), and average weight of 3 g were recorded. Six-month-old fish weaned on compound diet weighed three times more (90 vs 35 g) than their naturally fed counterparts

Effect of general anaesthesia on functional outcome in patients with anterior circulation ischaemic stroke having endovascular thrombectomy versus standard care: a meta-analysis of individual patient data

Background: General anaesthesia (GA) during endovascular thrombectomy has been associated with worse patient outcomes in observational studies compared with patients treated without GA. We assessed functional outcome in ischaemic stroke patients with large vessel anterior circulation occlusion undergoing endovascular thrombectomy under GA, versus thrombectomy not under GA (with or without sedation) versus standard care (ie, no thrombectomy), stratified by the use of GA versus standard care. Methods: For this meta-analysis, patient-level data were pooled from all patients included in randomised trials in PuMed published between Jan 1, 2010, and May 31, 2017, that compared endovascular thrombectomy predominantly done with stent retrievers with standard care in anterior circulation ischaemic stroke patients (HERMES Collaboration). The primary outcome was functional outcome assessed by ordinal analysis of the modified Rankin scale (mRS) at 90 days in the GA and non-GA subgroups of patients treated with endovascular therapy versus those patients treated with standard care, adjusted for baseline prognostic variables. To account for between-trial variance we used mixed-effects modelling with a random effect for trials incorporated in all models. Bias was assessed using the Cochrane method. The meta-analysis was prospectively designed, but not registered. Findings: Seven trials were identified by our search; of 1764 patients included in these trials, 871 were allocated to endovascular thrombectomy and 893 were assigned standard care. After exclusion of 74 patients (72 did not undergo the procedure and two had missing data on anaesthetic strategy), 236 (30%) of 797 patients who had endovascular procedures were treated under GA. At baseline, patients receiving GA were younger and had a shorter delay between stroke onset and randomisation but they had similar pre-treatment clinical severity compared with patients who did not have GA. Endovascular thrombectomy improved functional outcome at 3 months both in patients who had GA (adjusted common odds ratio (cOR) 1·52, 95% CI 1·09–2·11, p=0·014) and in those who did not have GA (adjusted cOR 2·33, 95% CI 1·75–3·10, p<0·0001) versus standard care. However, outcomes were significantly better for patients who did not receive GA versus those who received GA (covariate-adjusted cOR 1·53, 95% CI 1·14–2·04, p=0·0044). The risk of bias and variability between studies was assessed to be low. Interpretation: Worse outcomes after endovascular thrombectomy were associated with GA, after adjustment for baseline prognostic variables. These data support avoidance of GA whenever possible. The procedure did, however, remain effective versus standard care in patients treated under GA, indicating that treatment should not be withheld in those who require anaesthesia for medical reasons