Lusin-type approximation of Sobolev by Lipschitz functions, in Gaussian and RCD(K,∞)RCD(K,\infty) spaces

We establish new approximation results, in the sense of Lusin, of Sobolev functions by Lipschitz ones, in some classes of non-doubling metric measure structures. Our proof technique relies upon estimates for heat semigroups and applies to Gaussian and $RCD(K, \infty)$ spaces. As a consequence, we obtain quantitative stability for regular Lagrangian flows in Gaussian settings

Machining of Aluminum Alloy

Import 23/07/2015Diplomová práce se zabývá kvalitou opracované hliníkové slitiny EN AW-6082, speciálně drsností povrchu a měřením a vyhodnocením složek řezných sil. Teoretická část objasňuje základní pojmy věnované čelnímu frézování, obrobitelnosti hliníku, obráběným materiálům, řezným podmínkám a geometrii obrábění. V návrhu experimentální části práce je popsáno použití stroje, nástroje a vyměnitelných břitových destiček, přístrojů na měření drsnosti, velikosti řezných sil a navržené řezné podmínky. V experimentální části práce jsou změřeny drsnosti povrchu a presentovány výsledky naměřených hodnot drsnosti Ra a Rz. Řezné síly byly měřeny na piezoelektrickém dynamometru.This master thesis is concerned with the quality of machined aluminium alloy EN AW-6082, especially surface roughness and the measurement and evaluation components of the cutting forces. The theoretical part explains the basic concepts of frontal milling, machinability aluminium, machined material, cutting conditions and geometry processing. In the proposal of the experimental part is described the using of machine, tool and indexable inserts, devices for measuring roughness, cutting forces and proposed cutting conditions. In the experimental part of the work are measured surface roughnesses and presented the results of the measured values of roughness Ra and Rz. Cutting forces were measured on the piezoelectric dynamometer.346 - Katedra obrábění, montáže a strojírenské metrologievelmi dobř

Microfluidic Low-Input Fluidized-Bed Enabled ChIP-seq Device for Automated and Parallel Analysis of Histone Modifications

Genome-wide epigenetic changes, such as histone modifications, form a critical layer of gene regulations and have been implicated in a number of different disorders such as cancer and inflammation. Progress has been made to decrease the input required by gold-standard genome-wide profiling tools like chromatin immunoprecipitation followed by sequencing (i.e., ChIP-seq) to allow scarce primary tissues of a specific type from patients and lab animals to be tested. However, there has been practically no effort to rapidly increase the throughput of these low-input tools. In this report, we demonstrate LIFE-ChIP-seq (low-input fluidized-bed enabled chromatin immunoprecipitation followed by sequencing), an automated and high-throughput microfluidic platform capable of running multiple sets of ChIP assays on multiple histone marks in as little as 1 h with as few as 50 cells per assay. Our technology will enable testing of a large number of samples and replicates with low-abundance primary samples in the context of precision medicine

Immunofluorescence and FISH analysis of pachytene spermatocytes.

<p>(A) Spermatocytes were immunostained using antibodies against SYCP3/SYCP1, MLH1 and CREST to visualize the SC (red), crossover sites (green) and centromeres (blue). A spermatocyte with 45 crossovers from patient OA20 is shown. Although patient OA20 displayed normal rates of recombination, the crossover distribution on chromosome 18 was altered. We observed an increase in crossovers near the centromere and telomere on 18q and 18p, respectively. (B) Subsequent FISH was performed to identify chromosomes 13 (green, LSI 13), 18 (blue, CEP 18) and 21 (red, LSI 21) in the previously immunostained spermatocytes. (C) A spermatocyte from patient OA19 is shown. Although the patient showed normal rates of recombination, we observed an increased rate of synaptic errors compared to controls (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0156817#pone.0156817.t001" target="_blank">Table 1</a>). The unsynapsed region along a bivalent is indicated by the white arrow, where there is an absence of staining for SYCP3/SYCP1.</p

Microfluidic Low-Input Fluidized-Bed Enabled ChIP-seq Device for Automated and Parallel Analysis of Histone Modifications

Genome-wide epigenetic changes, such as histone modifications, form a critical layer of gene regulations and have been implicated in a number of different disorders such as cancer and inflammation. Progress has been made to decrease the input required by gold-standard genome-wide profiling tools like chromatin immunoprecipitation followed by sequencing (i.e., ChIP-seq) to allow scarce primary tissues of a specific type from patients and lab animals to be tested. However, there has been practically no effort to rapidly increase the throughput of these low-input tools. In this report, we demonstrate LIFE-ChIP-seq (low-input fluidized-bed enabled chromatin immunoprecipitation followed by sequencing), an automated and high-throughput microfluidic platform capable of running multiple sets of ChIP assays on multiple histone marks in as little as 1 h with as few as 50 cells per assay. Our technology will enable testing of a large number of samples and replicates with low-abundance primary samples in the context of precision medicine

Microfluidic Low-Input Fluidized-Bed Enabled ChIP-seq Device for Automated and Parallel Analysis of Histone Modifications

Genome-wide epigenetic changes, such as histone modifications, form a critical layer of gene regulations and have been implicated in a number of different disorders such as cancer and inflammation. Progress has been made to decrease the input required by gold-standard genome-wide profiling tools like chromatin immunoprecipitation followed by sequencing (i.e., ChIP-seq) to allow scarce primary tissues of a specific type from patients and lab animals to be tested. However, there has been practically no effort to rapidly increase the throughput of these low-input tools. In this report, we demonstrate LIFE-ChIP-seq (low-input fluidized-bed enabled chromatin immunoprecipitation followed by sequencing), an automated and high-throughput microfluidic platform capable of running multiple sets of ChIP assays on multiple histone marks in as little as 1 h with as few as 50 cells per assay. Our technology will enable testing of a large number of samples and replicates with low-abundance primary samples in the context of precision medicine

Microfluidic Low-Input Fluidized-Bed Enabled ChIP-seq Device for Automated and Parallel Analysis of Histone Modifications

Genome-wide epigenetic changes, such as histone modifications, form a critical layer of gene regulations and have been implicated in a number of different disorders such as cancer and inflammation. Progress has been made to decrease the input required by gold-standard genome-wide profiling tools like chromatin immunoprecipitation followed by sequencing (i.e., ChIP-seq) to allow scarce primary tissues of a specific type from patients and lab animals to be tested. However, there has been practically no effort to rapidly increase the throughput of these low-input tools. In this report, we demonstrate LIFE-ChIP-seq (low-input fluidized-bed enabled chromatin immunoprecipitation followed by sequencing), an automated and high-throughput microfluidic platform capable of running multiple sets of ChIP assays on multiple histone marks in as little as 1 h with as few as 50 cells per assay. Our technology will enable testing of a large number of samples and replicates with low-abundance primary samples in the context of precision medicine

Chromosomes 21 and 18 displaying altered crossover distributions in NOA men.

<p>Chromosome arms were divided into 10% intervals, and the crossover frequency in each interval was calculated. The Y-axis represents the frequency of crossovers in each interval. The X-axis represents the relative crossover position from the centromere with the values representing the upper limit of each interval. The centromere is labeled ‘C’ with the p-arm to the left and q-arm to the right. As crossovers in the p-arm of chromosome 21 are extremely rare, the p-arm is not shown. The black bars indicate the control group and the white bars indicate the individual NOA man. The crossover frequencies in each interval were compared to the control group and significant differences are indicated by asterisks (P < 0.05, Fisher test).</p

Analysis of crossover frequencies on chromosome 13, 18 and 21 in control and OA men.

<p>Analysis of crossover frequencies on chromosome 13, 18 and 21 in control and OA men.</p

Analysis of recombination and synaptic errors in control and OA men.

<p>Analysis of recombination and synaptic errors in control and OA men.</p