Quality of eggs produced from wild and captive spawners of Penaeus Indicus H. Milne Edwards and their bacterial load

The seeds of Penaeus indicus H. Milne Edwards were produced following the modified Galveston system at a hatchery located at Narakkal near Cochin, India. Unilaterally eyestalk-ablated wild females were used for seed production. Some adult prawns collected from grow-out ponds attached to the hatchery were also used for breeding purposes. In all, 20 spawnings were observed in the present study. Poor-quality eggs and abnormal nauplii were observed, usually from the pond-reared eyestalk-ablated females during the period of investigation. The present study was carried out to describe different types of eggs based on morphology and to determine the bacterial load of eggs and its relationship with hatching rate

Aquaculture and Marine Biotechnology Programme in India

Marine biotechnology has been a major focus world-wide through bio-discovery of marine microbes, invertebrates, micro-algae and macro-algae. In the commercial production of bio-active molecules and pharmaceuticals it plays a pivotal role. The global market for products from marine biotechnology is expected to reach over US$ 4 billion by 2015 and therefore marine bio-resources emerging with a lot of scope for the health and well being of aquacultur

Experimental transmission and histopathology of brown spot disease in shrimp (Penaeus indicus) and lobster (Panulirus homarus)

The occurrence of shell disease in prawn and lobster is reported. Black lesion was observed on abdominal appendages and telson of larvae and adult Penaeus indicus and adult Panulirus homarus. Bacterial isolates belonging to the genus Vibrio, especially V. alginolyticus, were isolated. Vibno alginolyticus caused black lesion on abdominal segments of larvae in experimental transmission. Histopathological changes were observed in hepatopancreas, gut, and muscle of infected animal

Bioencapsulation and Colonization Characteristics of Lactococcus lactis subsp. lactis CF4MRS in Artemia franciscana: a Biological Approach for the Control of Edwardsiellosis in Larviculture

Predominance of beneficial bacteria helps to establish a healthy microbiota in fish gastrointestinal system and thus to reduce emerging pathogen. In this study, the colonization efficacy of Lactococcus lactis subsp. lactis CF4MRS in Artemia franciscana and its potential as a probiotic in suppressing Edwardsiella sp. infection were investigated in vivo. The colonization extent of the bioencapsulated L. lactis was established through visualization of gfp gene-transformed L. lactis in A. franciscana. Here, we demonstrate that when A. franciscana is administrated with L. lactis at 108 CFU mL−1 for 8 h, the highest relative percentage of survival (RPS = 50.0) is observed after inoculation with Edwardsiella sp. The total counts of L. lactis entrapped in Artemia were the highest (ranged from 3.2 to 5.1 × 108 CFU mL−1), when 108–109 CFU mL−1 of L. lactis was used as starting inoculum, with the bioencapsulation performed within 8–24 h. Fluorescent microscopy showed gfp-transformed L. lactis colonized the external trunk surfaces, mid-gut and locomotion antennules of the A. franciscana nauplii. These illustrations elucidate the efficiency of colonization of L. lactis in the gastrointestinal tract and on the body surfaces of Artemia. In conclusion, L. lactis subsp. lactis CF4MRS shows a good efficacy of colonization in Artemia and has the potential for biocontrol/probiotic activity against Edwardsiella sp. infection

Studies on the pathobiology of penaeid larvae and postlarvae

Studies on the pathobiology of penaeid larvae and postlarvae. One of the major fac~ltors which influences the production and quality or seed in the hatchery and their subsequent culture in the grow-out system is tl!e uisczses and parasites affecting the stock. Under certain un fa-~surnble c~nditions~ diseases due to biotic and abiotic factors affect the iavYse ana postlarvae of penacid prawns. Further, these developing stages ale founu to be rrisre susceptible to diseases than the adult

Not Available

Not AvailableA Leptomonas like parasite was found to be responsible for mortality of larvae of Penaeus indicus and P. semisu2catus in the hatcheries. The mortality occurred in protozoea and mysis stages but not in naupliar and postlarval stages of prawn. The parasite exhibited polymorphism and three different forms were observed. It occurred in the appendages, eyestalks, eyes and rostrum of the host. Histological sections showed the presence of this parasite in internal organs such as hepatopancreas, gut and abdominal muscle.Not Availabl

Mortality in protozoea and mysis of Penaeus indicus and P. semisulcatus by Leptomonas like parasite in the hatcheries

A Leptomonas like parasite was found to be responsible for mortality of larvae of Penaeus indicus and P. semisu2catus in the hatcheries. The mortality occurred in protozoea and mysis stages but not in naupliar and postlarval stages of prawn. The parasite exhibited polymorphism and three different forms were observed. It occurred in the appendages, eyestalks, eyes and rostrum of the host. Histological sections showed the presence of this parasite in internal organs such as hepatopancreas, gut and abdominal muscle

Not Available

Not AvailableThe seeds of Penaeus indicus H. Milne Edwards were produced following the modified Galveston system at a hatchery located at Narakkal near Cochin, India. Unilaterally eyestalk-ablated wild females were used for seed production. Some adult prawns collected from grow-out ponds attached to the hatchery were also used for breeding purposes. In all, 20 spawnings were observed in the present study. Poor-quality eggs and abnormal nauplii were observed, usually from the pond-reared eyestalk-ablated females during the period of investigation. The present study was carried out to describe different types of eggs based on morphology and to determine the bacterial load of eggs and its relationship with hatching rate.Not Availabl

A comparative study of quality of eggs produced from wild and captive spawners of Penaeus indicus and their bacterial populations

Based on the morphology, the eggs of Penaeus indicus were classified into five different types : type A eggs with normal development; type B eggs with delayed or abnormal development; type C eggs with undifferentiated embryonic mass; type D eggs with unequal cells and type E unfertilised eggs with orange colour. The incidence of abnormal eggs was high in eyestalk ablated pond reared prawn. Statistical analysis revealed a significant difference in the percentage of abnormal eggs, hatching rate and bacterial numbers between eyestalk ablated pond reared and wild females of prawn. A significant correlation was found between the bacterial numbers and percentage of abnormal eggs, and bacterial numbers and hatching rate of eggs

A study of the aerobic heterotrophic bacterial flora of hatchery-reared eggs, larvae and post-larvae of Penaeus indicus

A study was carried out to investigate the aerobic heterotrophic bacterial flora of eggs, larvae and post-larvae of Penaeus indicus and larval rearing tank water in a hatchery using a modified Galveston rearing system. Physicochemical characteristics of larval rearing tank water, production history, and bacterial counts of eggs, nauplii, protozoeae, mysis and post-larvae were determined for three tanks in December 1986, January 1987 and February 1987. Temperature, pH, salinity and dissolved oxygen ranged from 27.4 to 31.3°C, 7.9 to 8.3, 30 to 32 ppt and 7.3 to 9.0 mg/l, respectively, during the period of investigation. Total viable bacterial counts ranged from 4.3×103 to 3.9×105 colony-forming units (cfu) per gram, 8.9×103 to 6.2×105 cfu/g, 8.1×104 to 3.7×106 cfu/g, 2.8×105 to 2.9×107 cfu/g and 9.5×105 to 1.2×108 cfu/g in the eggs, nauplii, protozoeae, mysis and post-larvae respectively and from 9.0×102 to 1.0×105 cfu/ml in the tank water. Vibrio sp. were found to be the dominant taxa in eggs, larvae and post-larvae, followed by Pseudomonas, Alcaligenes, Aeromonas and Flavobacterium, while Alcaligenes predominated in water samples, followed by Vibrio, Pseudomonas, Aeromonas and Flavobacteriu