Modulation of the hepatocyte rough endoplasmic reticulum single chloride channel by nucleotide-Mg 2+ interaction

The effect of nucleotides on single chloride channels derived from rat hepatocyte rough endoplasmic reticulum vesicles incorporated into bilayer lipid membrane was investigated. The single chloride channel currents were measured in 200/50 mmol/l KCl cis/trans solutions. Adding 2.5 mM adenosine triphosphate (ATP) and adenosine diphosphate (ADP) did not influence channel activity. However, MgATP addition inhibited the chloride channels by decreasing the channel open probability (Po) and current amplitude, whereas mixture of Mg 2+ and ADP activated the chloride channel by increasing the Po and unitary current amplitude. According to the results, there is a novel regulation mechanism for rough endoplasmic reticulum (RER) Cl - channel activity by intracellular MgATP and mixture of Mg 2+ and ADP that would result in significant inhibition by MgATP and activation by mixture of Mg 2+ and ADP. These modulatory effects of nucleotide-Mg 2+ complexes on chloride channels may be dependent on their chemical structure configuration. It seems that Mg-nucleotide-ion channel interactions are involved to produce a regulatory response for RER chloride channels. © Springer-Verlag 2012

The analysis of anticcp antibodies in the serum: a comparison between the patients with rheumatoid arthritis

Rheumatoid Arthritis (RA) is a chronic systemic autoimmune disease that causes inflammation, pain, stiffness and destructive changes in the joints. Although, Rheumatoid Factor (RF), has been the primary blood test used to detect RA, the anti-ccp antibodies detection test is a relatively new assay to detect the citrulline antibodies in blood. These autoantibodies are produced by immune system in response to a perceived threat of citrulline, an amino acid produced from arginine in the citrullination process. The objective of this study was to investigate the presence and prediction value of anti-ccp in RA patients and evaluate its sensitivity and specificity comparing to that of classic laboratory tests, CRP and RF. The serum of 84 patients with RA and 80 healthy control subjects were enrolled into the study. The anti-ccp, RF and CRP levels in the serums were assayed by ELISA and agglutination procedure, respectively. Our results provided evidence that anti-ccp level was significantly higher in patients with RA comparing to that of corresponding controls (p<0.0001). Anti-ccp was found to have the highest sensitivity and specificity (91%-91%) comparing to the other two tests (RF, CRP). The latter tests were found to have (97%- 92%) and (27%- 75%) sensitivity and specificity, respectively. The diagnostic value of anti-ccp is better than RF and CRP, individually. It can be detected early in the disease in unselected early arthritis patients. It is recommended to use RF test together with anti-ccp antibodies detection, in RA patients to ensure a higher diagnostic effectiveness

Dielectronic Recombination in Photoionized Gas. II. Laboratory Measurements for Fe XVIII and Fe XIX

In photoionized gases with cosmic abundances, dielectronic recombination (DR) proceeds primarily via nlj --> nl'j' core excitations (Dn=0 DR). We have measured the resonance strengths and energies for Fe XVIII to Fe XVII and Fe XIX to Fe XVIII Dn=0 DR. Using our measurements, we have calculated the Fe XVIII and Fe XIX Dn=0 DR DR rate coefficients. Significant discrepancies exist between our inferred rates and those of published calculations. These calculations overestimate the DR rates by factors of ~2 or underestimate it by factors of ~2 to orders of magnitude, but none are in good agreement with our results. Almost all published DR rates for modeling cosmic plasmas are computed using the same theoretical techniques as the above-mentioned calculations. Hence, our measurements call into question all theoretical Dn=0 DR rates used for ionization balance calculations of cosmic plasmas. At temperatures where the Fe XVIII and Fe XIX fractional abundances are predicted to peak in photoionized gases of cosmic abundances, the theoretical rates underestimate the Fe XVIII DR rate by a factor of ~2 and overestimate the Fe XIX DR rate by a factor of ~1.6. We have carried out new multiconfiguration Dirac-Fock and multiconfiguration Breit-Pauli calculations which agree with our measured resonance strengths and rate coefficients to within typically better than <~30%. We provide a fit to our inferred rate coefficients for use in plasma modeling. Using our DR measurements, we infer a factor of ~2 error in the Fe XX through Fe XXIV Dn=0 DR rates. We investigate the effects of this estimated error for the well-known thermal instability of photoionized gas. We find that errors in these rates cannot remove the instability, but they do dramatically affect the range in parameter space over which it forms.Comment: To appear in ApJS, 44 pages with 13 figures, AASTeX with postsript figure

Enhanced dielectronic recombination of lithium-like Ti19+ ions in external ExB fields

Dielectronic recombination(DR) of lithium-like Ti19+(1s2 2s) ions via 2s->2p core excitations has been measured at the Heidelberg heavy ion storage ring TSR. We find that not only external electric fields (0 <= Ey <= 280 V/cm) but also crossed magnetic fields (30 mT <= Bz <= 80 mT) influence the DR via high-n (2p_j nl)-Rydberg resonances. This result confirms our previous finding for isoelectronic Cl14+ ions [Bartsch T et al, PRL 82, 3779 (1999)] that experimentally established the sensitivity of DR to ExB fields. In the present investigation the larger 2p_{1/2}-2p_{3/2} fine structure splitting of Ti19+ allowed us to study separately the influence of external fields via the two series of Rydberg DR resonances attached to the 2s -> 2p_{1/2} and 2s -> 2p_{3/2} excitations of the Li-like core, extracting initial slopes and saturation fields of the enhancement. We find that for Ey > 80 V/cm the field induced enhancement is about 1.8 times stronger for the 2p_{3/2} series than for the 2p_{1/2} series.Comment: 10 pages, 3 figures, to be published in Journal of Physics B, see also http://www.strz.uni-giessen.de/~k

Dielectronic Recombination (via N=2 --> N'=2 Core Excitations) and Radiative Recombination of Fe XX: Laboratory Measurements and Theoretical Calculations

We have measured the resonance strengths and energies for dielectronic recombination (DR) of Fe XX forming Fe XIX via N=2 --> N'=2 (Delta_N=0) core excitations. We have also calculated the DR resonance strengths and energies using AUTOSTRUCTURE, HULLAC, MCDF, and R-matrix methods, four different state-of-the-art theoretical techniques. On average the theoretical resonance strengths agree to within <~10% with experiment. However, the 1 sigma standard deviation for the ratios of the theoretical-to-experimental resonance strengths is >~30% which is significantly larger than the estimated relative experimental uncertainty of <~10%. This suggests that similar errors exist in the calculated level populations and line emission spectrum of the recombined ion. We confirm that theoretical methods based on inverse-photoionization calculations (e.g., undamped R-matrix methods) will severely overestimate the strength of the DR process unless they include the effects of radiation damping. We also find that the coupling between the DR and radiative recombination (RR) channels is small. We have used our experimental and theoretical results to produce Maxwellian-averaged rate coefficients for Delta_N=0 DR of Fe XX. For kT>~1 eV, which includes the predicted formation temperatures for Fe XX in an optically thin, low-density photoionized plasma with cosmic abundances, our experimental and theoretical results are in good agreement. We have also used our R-matrix results, topped off using AUTOSTRUCTURE for RR into J>=25 levels, to calculate the rate coefficient for RR of Fe XX. Our RR results are in good agreement with previously published calculations.Comment: To be published in ApJS. 65 pages with 4 tables and lots of figure

Adenosine Deaminase 1 as a Biomarker for Diagnosis and Monitoring of Patients with Acute Lymphoblastic Leukemia

Background: Acute lymphoblastic leukemia (ALL) is known as the most prevalent pediatric malignancy all around the world. Identification of specific biomarker is necessary for early diagnosis and effective therapy. It is believed that Adenosine deaminase (ADA) as an enzyme involved in the purine salvage pathway increases in ALL patients. Herein, the quantity and pattern of ADA isoenzymes were surveyed among ALL patients in comparison to healthy subjects. Methods: Serum and RBC samples of three different groups of ALL patients, including newly diagnosed cases without any drugs administration, subjects with the relapsed disease, patients in the remission stage after therapy, and the healthy subjects were enrolled in the study. Then, the activity and pattern of ADA1, ADA2 and ADA1+cp were determined using ADA kit and electrophoresis on SDS-PAGE, respectively. To confirm the presence of ADA enzyme, the fresh serums, extractions from erythrocytes, JM cell line as a human T lymphocyte line and J774 A.1 as mouse monocyte line were electrophoresed on 1.2 agarose gel and stained with the specific dye. Results: The activities of ADA1 isoenzyme and total ADA in new cases and subjects with the relapsed disease were significantly higher than their activities in the patients in the remission stage and healthy controls (p<0.001). The unbounded ADA1 isoenzyme was found to exist in the erythrocyte, lymphocyte and monocyte. But in serum, all the ADA1 was bounded to the cp protein. Conclusions: ADA1 is the key isoenzyme elevating in ALL patients, therefore this isoenzyme could be a useful biomarker to diagnose ALL patients and monitor their therapies. © 2017 Mina Ebrahimi-Rad et al

Dielectronic Recombination of Ground-State and Metastable Li+ Ions

Dielectronic recombination has been investigated for Delta-n = 1 resonances of ground-state Li+(1s^2) and for Delta-n = 0 resonances of metastable Li+(1s2s ^3S). The ground-state spectrum shows three prominent transitions between 53 and 64 eV, while the metastable spectrum exhibits many transitions with energies < 3.2 eV. Reasonably good agreement of R-matrix, LS coupling calculations with the measured recombination rate coefficient is obtained. The time dependence of the recombination rate yields a radiative lifetime of 52.2 +- 5.0 s for the 2 ^3S level of Li+.Comment: Submitted to Phys. Rev. A; REVTeX, 4 pages, 3 figure

Adenosine Deaminase 1 as a Biomarker for Diagnosis and Monitoring of Patients with Acute Lymphoblastic Leukemia

Background: Acute lymphoblastic leukemia (ALL) is known as the most prevalent pediatric malignancy all around the world. Identification of specific biomarker is necessary for early diagnosis and effective therapy. It is believed that Adenosine deaminase (ADA) as an enzyme involved in the purine salvage pathway increases in ALL patients. Herein, the quantity and pattern of ADA isoenzymes were surveyed among ALL patients in comparison to healthy subjects. Methods: Serum and RBC samples of three different groups of ALL patients, including newly diagnosed cases without any drugs administration, subjects with the relapsed disease, patients in the remission stage after therapy, and the healthy subjects were enrolled in the study. Then, the activity and pattern of ADA1, ADA2 and ADA1+cp were determined using ADA kit and electrophoresis on SDS-PAGE, respectively. To confirm the presence of ADA enzyme, the fresh serums, extractions from erythrocytes, JM cell line as a human T lymphocyte line and J774 A.1 as mouse monocyte line were electrophoresed on 1.2 agarose gel and stained with the specific dye. Results: The activities of ADA1 isoenzyme and total ADA in new cases and subjects with the relapsed disease were significantly higher than their activities in the patients in the remission stage and healthy controls (p<0.001). The unbounded ADA1 isoenzyme was found to exist in the erythrocyte, lymphocyte and monocyte. But in serum, all the ADA1 was bounded to the cp protein. Conclusions: ADA1 is the key isoenzyme elevating in ALL patients, therefore this isoenzyme could be a useful biomarker to diagnose ALL patients and monitor their therapies. © 2017 Mina Ebrahimi-Rad et al

Dielectronic Recombination of Fe XIX Forming Fe XVIII: Laboratory Measurements and Theoretical Calculations

We have measured resonance strengths and energies for dielectronic recombination (DR) of Fe XIX forming Fe XVIII via N = 2 → N' = 2 and N = 2 → N' = 3 core excitations. All measurements were carried out using the heavy-ion Test Storage Ring at the Max Planck Institute for Nuclear Physics in Heidelberg, Germany. We have also calculated these resonance strengths and energies using two independent, state-of-the-art techniques: the perturbative multiconfiguration Breit-Pauli (MCBP) and multiconfiguration Dirac-Fock (MCDF) methods. Overall, reasonable agreement is found between our experimental results and theoretical calculations. The most notable discrepancies are for the 3l3l' resonances. The calculated MCBP and MCDF resonance strengths for the n = 3 complex lie, respectively, ≈47% and ≈31% above the measured values. These discrepancies are larger than the estimated ≲ 20% total experimental uncertainty in our measurements. We have used our measured 2 → 2 and 2 → 3 results to produce a Maxwellian-averaged rate coefficient for DR of Fe XIX. Our experimentally derived rate coefficient is estimated to be good to better than ≈20% for kBTe ≥ 1 eV. Fe XIX is predicted to form in photoionized and collisionally ionized cosmic plasmas at kBTe Gt 1 eV. Hence, our rate coefficient is suitable for use in ionization balance calculations of these plasmas. Previously published theoretical DR rate coefficients are in poor agreement with our experimental results. None of these published calculations reliably reproduce the magnitude or temperature dependence of the experimentally derived rate coefficient. Our MCBP and MCDF results agree with our experimental rate coefficient to within ≈20%