35P Wstępne wyniki kontroli radioterapii techniką zdjęć sprawdzających

Cel pracyWeryfikacja ułożenia pacjenta na aparacie terapeutycznym techniką zdjęć sprawdzających.Materiał i metodyZdjęcia sprawdzające wykonano dla pacjentów leczonych w Świętokrzyskim Centrum Onkologii w Kielcach a powodu nowotworu1)krtani (obszar głowy i szyi), napromienianych techniką dwóch pól przeciwległych; pacjent unieruchomiony maską orfitową2)z obszaru miednicy, napromienianych techniką box; pacjent unieruchomiony maską z pelvicastu oraz bez takiej maski.Zdjęcia wykonano na filmach Kodak X-Omatic V film pelicula (for therapy veryfication) w kasetach Kodak X-Omatic cassette V Radiation Therapy for Portal Verification.Zdjęcia portalowe porównano ze zdjęciami z symulatora, które zostały poddane obróbce cyfrowej przy użyciu programu komputerowego.Photoipact 3.0 SE. Na zdjęciach portalowych wyznaczono środki pól promieniowania, a następnie nałożono zdjęcia portalowe i z symulatora. Nałożenie polegało na dopasowaniu struktur kostnych, dla których przyjęto, że nie zmieniają swojego położenia podczas całego procesu leczenia.Jako miarę powtarzalności napromieniania przyjęto różnice położenia pomiędzy środkami pól oraz kąt pomiędzy płaszczyznami głównymi dla zdjęć portalowych i z symulatora.WynikiŚrednia wartość przesunięcia środka pola [mm] ze zdjęcia portalowego względem środka pola z symulatora w kierunku X (poprzeczny), Y (wzdłuż osi ciała), Z (A-P)oraz odpowiadającego im odchylenia standardowego są następujące:1)krtań (strona lewa): y=0,6, z=0,9 δy=0,47, δz=0,76;2)miednica (z pelvicastem AP):x=3,2, y=3,1, δx=1,32, δy=2,013)miednica (bez pelvicastu AP): x=0,2, y=0,1 δx=1,20, δy=0,89.PodsumowanieWstępne wyniki wskazują na bardzo dobrą powtarzalność dla pacjentów z nowotworem regionu głowy i szyi oraz dobrą powtarzalność dla pacjentów z nowotworem w rejonie miednicy

Variations in popliteal fossa venous anatomy: implications for diagnosis of deep-vein thrombosis

Background: To retrospectively review the bilateral venous system within the popliteal fossa to evaluate the types of variations and their frequency seen in venous anatomy.Materials and methods: During routine dissection of formalin-fixed cadavers, a retrospective review of 32 bilateral (64 limbs) lower limbs obtained from adult donors was performed. Deep veins present in the popliteal fossa were evaluated according to predetermined criteria for the presence of duplication of vessels and interindividual variations in venous anatomy.Results: More than one deep venous vessel was seen in the popliteal fossa in 20 (31.3%) of 64 limbs. In 12 (18.7%) cases there was a high (just below the level of the adductor hiatus) origin of the popliteal vein: from 2 tributaries in 10 (15.6%) and 3 tributaries in 2 (3.1%). In 5 (7.8%) cases true duplicated popliteal veins were observed. There were also 3 (4.7%) cases, including one bilateral, of persistent sciatic vein.Conclusions: Variations in popliteal fossa venous anatomy are common and have important implications for the diagnosis of deep vein thrombosis

Metro-Line Crossing Minimization: Hardness, Approximations, and Tractable Cases

Crossing minimization is one of the central problems in graph drawing. Recently, there has been an increased interest in the problem of minimizing crossings between paths in drawings of graphs. This is the metro-line crossing minimization problem (MLCM): Given an embedded graph and a set L of simple paths, called lines, order the lines on each edge so that the total number of crossings is minimized. So far, the complexity of MLCM has been an open problem. In contrast, the problem variant in which line ends must be placed in outermost position on their edges (MLCM-P) is known to be NP-hard. Our main results answer two open questions: (i) We show that MLCM is NP-hard. (ii) We give an $O(\sqrt{\log |L|})$-approximation algorithm for MLCM-P

Plasma medicine: The great prospects when physics meets medicine

The research has demonstrated the antimicrobial properties of plasma urging the incorporation of cold atmospheric plasma (CAP) decontamination in current clinical therapies with the aim to improve the benefits on the patients and on society.Postprint (published version

Selection of antibody single-chain variable fragments with improved carbohydrate binding by phage display.

A single-chain variable fragment (Fv) version of a murine monoclonal antibody, Se155-4, specific for Salmonella serogroup B O-polysaccharide, was used as a model system for testing monovalent phage display as a route for enhancing the relatively low affinities that typify anti-carbohydrate antibodies. Random single-chain Fv mutant libraries generated by chemical and error-prone polymerase chain reaction methods were panned against the serogroup B lipopolysaccharide. Panning of a randomly mutated heavy chain variable domain library indicated selection for improved serogroup B binders and yielded six mutants, five of which showed wild type activity by enzyme immunoassay. Two of these were apparently selected on the basis of better functional single-chain Fv yield in Escherichia coli. A heavy chain mutation (Ile77-->Thr) in one mutant, 3B1, appeared to have a particularly dramatic effect, resulting in yields of approximately 120 mg/liter of functional periplasmic product. The sixth mutant, 4B2, had complementarity determining region 1 (CDR1) and CDR2 mutations and demonstrated 10-fold improved binding, by enzyme immunoassay, relative to the wild type. Extensive analysis of antigen-antibody interactions indicated that the improved binding properties of 4B2 were attributable to a higher association rate constant and interaction with an epitope that is larger than the trisaccharide recognized by the wild type. None of the mutations involved known trisaccharide contact residues; this was consistent with analysis of wild type and mutant single-chain Fvs by titration microcalorimetry. Examination of the structure indicated that two mutations in the heavy chain CDR2 provided improved surface complementarity between the protein and the extended epitope encompassing 2 additional hexose residues. However, introduction of only the CDR2 mutations into the wild type structure failed to confer the improved binding properties of 4B2, indicating an indirect effect by the more distant mutations. Panning of randomly mutated light chain variable domain and full-length single-chain Fv mutant libraries did not yield mutants with improved assembly or binding properties

Neutralizing Anti-Interleukin-1β Antibodies Reduce Ischemia-Related Interleukin-1β Transport Across the Blood-Brain Barrier in Fetal Sheep

Hypoxic ischemic insults predispose to perinatal brain injury. Pro-inflammatory cytokines are important in the evolution of this injury. Interleukin-1β (IL-1β) is a key mediator of inflammatory responses and elevated IL-1β levels in brain correlate with adverse neurodevelopmental outcomes after brain injury. Impaired blood-brain barrier (BBB) function represents an important component of hypoxic-ischemic brain injury in the fetus. In addition, ischemia-reperfusion increases cytokine transport across the BBB of the ovine fetus. Reducing pro-inflammatory cytokine entry into brain could represent a novel approach to attenuate ischemia-related brain injury. We hypothesized that infusions of neutralizing IL-1β monoclonal antibody (mAb) reduce IL-1β transport across the BBB after ischemia in the fetus. Fetal sheep were studied 24-h after 30-min of carotid artery occlusion. Fetuses were treated with placebo- or anti-IL-1β mAb intravenously 15-min and 4-h after ischemia. Ovine IL-1β protein expressed from IL-1β pGEX-2T vectors in E. Coli BL-21 cells was produced, purified, and radiolabeled with 125I. BBB permeability was quantified using the blood-to-brain transfer constant (Ki) with 125I-radiolabeled-IL-1β. Increases in anti-IL-1β mAb were observed in the brain of the mAb-treated group (P \u3c 0.001). Blood-to-brain transport of 125I-IL-1β was lower (P \u3c 0.04) across brain regions in the anti-IL-1β mAb treated than placebo-treated ischemic fetuses. Plasma 125I-IL-1β counts were higher (P \u3c 0.001) in the anti-IL-1β mAb than placebo-treated ischemic fetuses. Systemic infusions of anti-IL-1β mAb reduce IL-1β transport across the BBB after ischemia in the ovine fetus. Our findings suggest that conditions associated with increases in systemic pro-inflammatory cytokines and neurodevelopmental impairment could benefit from an anti-cytokine therapeutic strategy

Bacterial expression and secretion of various single-chain Fv genes encoding proteins specific for a Salmonella serotype B O-antigen.

Active single-chain Fv molecules encoded by synthetic genes have been expressed and secreted to the periplasm of Escherichia coli using the ompA secretory signal. Four different constructs were developed to investigate the effects of peptide linker design and VL-VH orientation on expression, secretion, and binding to a Salmonella O-polysaccharide antigen. Peptide linker sequences derived from the elbow regions of the Fab molecule were used alone or in combination with the flexible (GGGGS)2 sequence. VL and VH domain order in the single chain molecules had a profound effect on the level of secretion but hardly influenced total expression levels, which were approximately 50 mg/liter, chiefly in the form of inclusion bodies. With VL in the NH2-terminal position, the amount of secreted product obtained was 2.4 mg/liter, but when VH occupied this position the yield was less than 5% of this value. Enzyme immunoassays of the four products showed domain order and linker sequence affected antigen binding by less than an order of magnitude. Attempts to express active Fv from dicistronic DNA were unsuccessful, but active Fv was obtained from single-chain Fv by enzymic cleavage at a site in the elbow linker peptide. The thermodynamic binding parameters of intact and cleaved single-chain Fvs determined by titration microcalorimetry were similar to those of bacterially produced Fab and mouse IgG

CALIBRATION AND APPLICATION OF NUCLEAR TRACK DETECTORS FOR HIGH-TEMPERATURE PLASMA DIAGNOSTICS

Abstract The paper reports on features of so-called solid-state nuclear track detectors (SSNTDs), their calibration measurements performed with known ion beams, and their different applications for detailed studies of charged particle emissions from various high-temperatures plasma facilities