Intranasal insulin treatment improves memory and learning in a rat amyloid-beta model of Alzheimer’s disease

Recently, insulin has been used as a pro-cognitive agent for the potential treatment of Alzheimer’s disease (AD), because of its ability to cross the brain–blood barrier (BBB) by a saturable transport system. This study has been designed to evaluate the effects of intranasal insulin regimen, as a bypass system of BBB, on spatial memory in amyloid-beta (Aβ) model of AD in rat. Unilateral infusion of Aβ25–35 (10 nmol/2 µl/rat) into the lateral ventricular region of brain was used to produce a rat model of AD. After a 24-h recovery period, rats received insulin or vehicle via intraperitoneal or intranasal route (0.1, 0.2, and 0.3 IU) for 14 days. Memory function in rats was assessed by Morris water maze test, with 5 days of training and consequent probe test protocol. Different doses of intraperitoneal insulin did not have a significant effect on learning and memory in AD rats. However, intranasal insulin at doses of 0.2 and 0.3 IU improved the learning and memory in Aβ-received rats. In conclusion, intranasal insulin as a non-invasive strategy improves spatial learning and memory in AD model

Comparative survey on anesthetizing effects of medicinal herbs Valerian officinalis, Melissa officinalis, Papaver somniferum, and Papaver bracteatum on gold fish (Carassius auratus)

Anesthetic drugs are widely used aquaculture farms and can create consumption problems for humans, so there is a great need to safer drugs. With regard to long record of herbal drugs consumption in Iran and their benefits, we survey the possibility of using Valerian officinalis, Melissa officinalis, Papaver somniferum, and P. bracteatum as fish anesthetic. We provided, processed, and made consistent extractions of V. officinalis (2, 3 4g/lit), M. officinalis (5, 10, 15g/lit), P. somniferum (0.85, 1.6, 3.2g/lit) and P. bracteatum (0.3, 0.6, 0.9g/lit). We selected 60 goldfish Carassius auratus in the weight range of 7.41 plus or minus 0/2g, and the length range of 8.4 plus or minus 0/11cm and kept them in laboratory conditions under the same oxygen and temperature. The fish were divided into four groups each containing 15 fish and further into three subgroups of five fish each. During the experiments, two herbs P. somniferum and P. bracteaturn were eliminated from statistical analysis because of biased results

Proton-Coupled Electron-Transfer Mechanism for the Radical Scavenging Activity of Cardiovascular Drug Dipyridamole

Dipyridamole (DIP) is a well-known pharmaceutical drug used as a coronary vasodilator and anti-platelet agent in clinics for treating several cardiovascular diseases. Primarily, the therapeutic effects of the drug are attributed to its antioxidant potency. In this research, we aim to declare the unknown antioxidant mechanism of DIP as well as its potent chain-breaking antioxidant activity in polar aqueous medium inside the cells, using different experimental methods and theoretical quantum calculations. Data demonstrated the higher antioxidant capacity of DIP against ROS and free radicals in polar cell's interior. DIP is capable of generating long living and noninvasive DIP• radicals in oxidant condition that leads to an effective “chain-breaking antioxidant” activity. Quantum computational data indicated that DIP antioxidant has more favorable ionization potential than trolox which means DIP has higher antioxidant activity. Also, data showed that the direct hydrogen-transfer is not a favorable process to construct DIP• because of high barrier energy, though electron-transfer process can more easily to produce DIP•+ with the lowest barrier energy. Altogether, the electron donating potency of DIP to reduce ferric ion, having the low anodic oxidation peak potential, producing long lived stable DIP• radicals and protecting myoblast cells from oxidation, proposed the excellent “chain-breaking antioxidant” potency via electron-transfer mechanism of this vasodilator DIP drug in polar aqueous medium

D-galactose-induced brain ageing model:A systematic review and meta-analysis on cognitive outcomes and oxidative stress indices

Animal models are commonly used in brain ageing research. Amongst these, models where rodents are exposed to d-galactose are held to recapitulate a number of features of ageing including neurobehavioral and neurochemical changes. However, results from animal studies are often inconsistent. To better understand the characteristics of the model and effects of d-galactose on neurobehavioral and neurochemical outcomes in rodents we performed a systematic review and meta-analysis. We applied random-effects meta-analysis to evaluate the effect of study features. Our results give an overview of the characteristics of the d-galactose rodent ageing model, including neurobehavioral and neurochemical outcomes. We found that few studies took measures to reduce risks of bias, and substantial heterogeneity in the reported effects of d-galactose in included studies. This highlights the need for improvements in the use of the d-galactose rodent ageing model if it is to provide useful in the development of drugs to treat human ageing

Survey of Ergosan additive effects on gain weight, perpetuity rate and food conversion index in Rainbow trout

Evaluation of the effect of Ergosan additive on growth rate, feed conversion index and survival rate of 1gr Rainbow trout hatchlings. The purpose of the present study was to evaluate the effect of consumption of Ergosan additive on production indices of 1gr Rainbow trouts namely growth rate, feed conversion index and survival rate. Thus the effect of this additive was investigated by selecting five experimental groups (one control and four treatment groups) each consisting of 1500 fish with a weight of 1gram reared in exactly identical conditions. The treatment groups were fed 1, 2, 3 and 4 kg/ton of Ergosan with their diet for 60 days and the data were then analyzed. According to the results, Ergsan had the best effect on the production indices of the third treatment group (3 kg/ton) (

MicroRNA-4422-5p as a Negative Regulator of Amyloidogenic Secretases: A Potential Biomarker for Alzheimer's Disease

Beta-secretase (BACE1) and gamma-secretase activating protein (GSAP) are pivotal enzymes in the cleavage of amyloid precursor protein (APP). Beta-amyloid (A�) formation is considered one of the main reasons for Alzheimer's disease (AD) pathology. In our preliminary study, a series of microRNAs (miRs) with possible interaction with BACE1 and/or GSAP was selected using computational analysis. Our results showed that miR-4422-5p had a reduced level in the serum of AD patients. In this study, the effect of miR-4422-5p using miR-4422-5p mimic and inhibitor on BACE1 and GSAP were investigated, and a probable novel early diagnostic marker for AD was introduced. The effect of miR-4422-5p interaction with BACE1 and GSAP was evaluated via in vitro experiments using dual-luciferase assays, western blotting, and Immunocytochemistry. Luciferase assay demonstrated that miR-4422-5p mimic suppresses BACE1 and GSAP expression by directly targeting the 3�UTR of BACE1 and GSAP mRNA in HEK293T cells. Also, western blotting and immunocytochemistry confirmed the regulatory role of miR-4422-5p mimic on BACE1 and GSAP genes. miR-4422-5p mimic significantly decreased BACE1 and GSAP protein expression in SH-SY5Y and A549 cells, respectively. Moreover, miR-4422-5p-inhibitor reversed the expression processes in both cell lines. Our data suggest that miR-4422-5p may be an important regulator of both BACE1 and GSAP genes and can represent a novel potential biomarker or therapeutic target in AD. © 2021 IBR

Immunogenicity of commercial, formaldehyde and binary ethylenimine inactivated Newcastle disease virus vaccines in specific pathogen free chickens

Newcastle disease (ND) is one of the most important diseases that affect birds; the epizootic nature of the disease has caused severe economic losses in the poultry industry worldwide. In this experiment ND virus (NDV) was inactivated by two different chemicals binary ethylenimine (BEI) and formaldehyde. Formaldehyde was used at 0.1%, while BEI was used at concentrations of 1 to 4 mM. NDV inactivation with BEI was done in various incubation temperatures and periods and the best result (30 °C, 4 mM BEI and 21 hrs treatment) used as an experimental vaccine. Prepared inactivated NDV vaccines and a commercial vaccine were tested for their efficiency in generating humoral immune response in different groups of specific pathogen free (SPF) chicks. Test groups received 0.2 ml formaldehyde inactivated NDV (NDVF), BEI inactivated NDV (NDVEI) and Razi institute produced NDV vaccine (NDVR) subcutaneously respectively. HI Log 2 total mean titer of NDVEI group (8.42 ± 0.12) were significantly higher than NDVF (7.64 ± 0.16) and NDVR (7.86 ± 0.11) groups (p<0.05). BEI-inactivated vaccine gave higher antibody titers than formaldehyde-inactivated vaccine and preserves both structural integrity and antigenicity of the virus. Thus, it might be possible to use these compounds as an inactivator agent for commercial NDV inactivated vaccines in future