FABRICATION OF NOVEL ANTICANCER POLYOXOMETALATE [CoW11O39(CpTi)]7- -CHITOSAN NANO-COMPOSITE, ITS TOXICITY REDUCTION AND SUSTAINED RELEASE

Objectives: Polyoxometalates (POMs) are proved to be important for applications in medicine and in material science. Here, we representnanocomposite formation of tungsten-containing potent anticancer polyanion, K 6 H [CoW 11 O 39  (CpTi)].13H 2 O (CoW CpTi) with biocompatible ChitosanYC-100 (CSYC100) with the goal to reduce its heavy metal toxicity.Methods: Synthesis of POM-CSYC100 nanocomposite†was attained without the aid of any cross-linker through electrostatic interaction technique. Nanocomposites were characterized using Fourier transform infrared spectroscopy, dynamic light scattering, transmission electron microscopy, and thermogravimetric analysis. The release profile recorded was slow and sustained at physiological pH. In vitro cytotoxicity assays which show an attribute to reduce the toxicity of these POM were performed on C2C12 (mouse myoblast cell line) and A-549 (lung cancer cell line), which proved the reduced toxicity of nanocomposites as compared to the bare drugs.Results: Sustained release studies showed there was a slow and steady release of CoW CpTi for 11 hrs, with the 98% of collective release at the end. From in vitro cytotoxic assay, it was deduced that CoW 11 11 CpTi -CSYC100 nanocomposite at the concentrations of 1.25 mM, and lower did not exhibit toxic effect on C2C12 cells as 95% total C2C12 cell mass remained viable. While in the case of A549 cells highest 5 mM concentration of bare CoW 11 CpTi is toxic to the cancer cells and after encapsulation cell viability increases from 10% to 55%.Conclusion: Thus, this study has designated the probability of using POM-chitosan nanocomposite for less toxic and effective biomedicinal applications.Keywords: Anticancer, Chitosan, Nanocomposite, In vitro cytotoxicity, Drug release

Large Amplitude Oscillatory Shear Study of a Colloidal Gel at the Critical State

We investigate the nonlinear viscoelastic behavior of a colloidal dispersion at the critical gel state using large amplitude oscillatory shear (LAOS) rheology. The colloidal gel at the critical point is subjected to oscillatory shear flow with increasing strain amplitude at different frequencies. We observe that the first harmonic of the elastic and viscous moduli exhibits a monotonic decrease as the material undergoes a linear to nonlinear transition. We analyze the stress waveform across this transition and obtain the nonlinear moduli and viscosity as a function of frequency and strain amplitude. The analysis of the nonlinear moduli and viscosities suggests intracycle strain stiffening and intracycle shear thinning in the colloidal dispersion. Based on the insights obtained from the nonlinear analysis, we propose a potential scenario of the microstructural changes occurring in the nonlinear region. We also develop an integral model using the time-strain separable K-BKZ constitutive equation with a power-law relaxation modulus and damping function obtained from experiments. At low strain amplitudes, this model compares well with experimental data at all frequencies. However, a stronger damping function, which can be efficiently inferred using a spectral method, is required to obtain quantitative fits across the entire range of strain amplitudes and the explored frequencies.Comment: 34 pages, 10 figure

Stability and Dynamics of Complex Order Fractional Difference Equations

We extend the definition of $n$-dimensional difference equations to complex order $\alpha\in \mathbb{C}$. We investigate the stability of linear systems defined by an $n$-dimensional matrix $A$ and derive conditions for the stability of equilibrium points for linear systems. For the one-dimensional case where $A =\lambda \in \mathbb {C}$, we find that the stability region, if any is enclosed by a boundary curve and we obtain a parametric equation for the same. Furthermore, we find that there is no stable region if this parametric curve is self-intersecting. Even for $\lambda \in \mathbb{R}$, the solutions can be complex and dynamics in one-dimension is richer than the case for $\alpha\in \mathbb{R}$. These results can be extended to $n$-dimensions. For nonlinear systems, we observe that the stability of the linearized system determines the stability of the equilibrium point.Comment: 21 pages, 17 figure

A RARE CASE SCENARIO- ASSOCIATION OF POSTEROLATERAL OSTEOCHONDRAL INJURY WITH PCL AVULSION, ACL TEAR AND LATERAL MENISCUS INJURY

Association of posterolateral osteochondral (OCD) injury with multi- ligamentous knee injury (MLKI) can be a devastating injury which often results in long-term knee instability, loss of function and early osteoarthritis. For such patients, paucity of literature persists on management of such injury for better outcome. This case critically demonstrates an attempt to manage the patient with such rare scenario with the best options for early recovery

Survey on Wireless Intelligent Video Surveillance System Using Moving Object Recognition Technology

Video cameras are becoming a ubiquitous feature of modern life, useful for surveillance, crime prevention, and forensic evidence. We cannot solely rely upon human efforts to watch and shift through hundreds and thousands of video frames for crime alerts and forensic analysis. That is a non scalable task. We need a semi automated video analysis and event recognition system that can provide timely warnings to alert security personnel, and that can substantially reduce the search space for forensic analysis tasks. This survey describes the approach of wireless intelligent video surveillance system using moving object recognition technique. Keywords: Wireless, Video surveillance, moving object recognitio

Evaluation of used eye drop containers for microbial contamination in outpatient department of tertiary care teaching hospital

Background: Contaminated eyedrops are considered as serious risk factor for many iatrogenic ocular infections. Apart from the risk of infection, microbial contamination may alter the pH of the solution thereby reducing the efficacy of drugs. Presently many preservatives are added to these eye drops preparations to extend the duration of use. Hence authors aimed this study to find the contamination rates in such eye drop preparations.Methods: This was a prospective observational research conducted at Ophthalmology OPD, of tertiary care teaching hospital for the period of 2 months. Total fifty five used eyedrops were collected.Results: Authors found that 25.45% of the collected eye drops were contaminated with various organisms, viz. E. coli (10.90%), Staphylococcus aureus (9.09%), Pseudomonas aerugenosa (1.81%), Bacillus subtilis (1.81%) and Candida albicans (1.81%). Among various eyedrops, mydriatic (60%) eyedrops had the highest rate of contamination. We also found that, different preservatives in the eye drops were presents with different level of microbial contamination.Conclusions: The present study showed that there is a definite co-occurrence between eyedrop contamination and ocular infections irrespective of preservatives. This research raises a concern about questionable efficacy of preservatives.

A narrative review comparing clinical effectiveness of commonly used uterine balloon tamponade devices for postpartum haemorrhage management in India

 Background: World Health Organisation recommends using Uterine Balloon Tamponade (UBT) for refractory atonic postpartum haemorrhage (PPH) management provided treatment protocols and surgical recourse is possible. Methods: This review collated literature from three electronic databases between January 2010 to December 2019 to compare clinical effectiveness, safety and use related parameters for condom-UBT, Bakri balloon and Every Second Matters (ESM) UBT devices used in India. Results: Thirty-three eligible studies reported effectiveness in managing all PPH causes ranging from 84.2% to 98.3% for condom-UBT and from 65.3% to 94.8% for Bakri-UBT. Three ESM-UBT studies reported PPH survival rates of 94% to 97.4%. Mean UBT effectiveness in controlling atonic PPH was 92.3% for condom-UBT, 84.3% for Bakri-UBT and 97.3% for ESM-UBT. Condom-UBT and Bakri-UBT were comparable across parameters whereas limited ESM-UBT evidence reported success in preventing maternal deaths. Conclusions: For limitations and heterogeneity in methodology and outcome parameters with existing evidence, a robust comparative RCT between UBT devices in India is recommended

Low-pressure Chemical Vapour Deposition of Silicon Nanoparticles:Synthesis and Characterisation

emiconductor nanostructures such as quantum wells, quantum wires or quantum dots exhibit superior properties in comparison to their bulk forms. Quantum dots are described aszero-dimensional electron gas system, as carriers are confined in all the three directions. Densityof states is discrete function of energy. Allowed energy spectrum is discrete like in an atom.Energy band gap is broadened due to carriers confinement. Semiconductor quantum dots exhibittypical coulomb blockade characteristic which is exploited for development of new generationof nanoelectronic devices namely single-electron transistor, memories, etc, whose operationdepends on quantum mechanical tunneling of carriers through energy barriers. Thesesemiconductor nanostructures emit light in visible range upon excitation by optical means. Inrecent years,  research  has been focused on different nano-scale materials; metals (Au, Ag, Fe,Mn, Ni), metal oxides (SnO2, ZnO2), compound semiconductors (GaAs, GaAlAs, CdSe, CdS,GaN), and elemental semiconductors (silicon and germanium). As silicon is the most favouredmaterial in the established integrated circuits manufacturing technology, research is being donefor controlled synthesis and characterisation of Si nanoparticles. The Si nanoparticles havebeen synthesised on oxide and nitride layers over  Si substrate by IC technology compatiblelow-pressure chemical vapour deposition technique. Atomic force microscopy (AFM)characterisation has been extensively carried out on the samples. It is shown that the tip radiusand shape of tip lead to less accurate estimate of the actual size. The AFM images have been evaluated based on the real surface topography and shape of the tip. Photolumine scence (PL) studies have been performed to characterise the samples. The PL measurements showed visiblelight emission from synthesised silicon nanoparticles.Defence Science Journal, 2008, 58(4), pp.550-558, DOI:http://dx.doi.org/10.14429/dsj.58.167