HUBUNGAN ANTARA PENGUNGKAPAN DIRI MELALUI MEDIA SOSIAL WHATSAPP DENGAN KOMUNIKASI INTERPERSONAL PADA SISWA SEMESTER EMPAT SMA NEGERI 1 SALATIGA

Pengungkapan diri adalah proses membagikan informasi pribadi kepada orang lain. Komunikasi interpersonal merupakan proses komunikasi tatap muka yang dilakukan antara dua orang atau lebih yang menghasilkan respon baik verbal mapun nonverbal. Perkembangan teknologi khususnya media sosial saat ini membuat banyaknya individu khususnya remaja yang beralih menggunakan media sosial sebagai media pengungkapan diri. Penelitian ini bertujuan untuk mengetahui ada atau tidaknya hubungan antara pengungkapan diri melalui media sosial dengan komunikasi interpersonal pada remaja. Subjek dari penelitian ini adalah siswa semester empat di SMA Negeri 1 Salatiga. Populasi berjumlah 284 siswa dengan sampel penelitian sebanyak 161 siswa. Teknik pengambilan data pada penelitian ini menggunakan teknik cluster random sampling. Metode pengumpulan data menggunakan skala komunikasi interpersonal (27 aitem valid, α = .853) dan pengungkapan diri melalui media sosial WhatsApp (31 aitem valid, α = .895). Hasil analisis data menggunakan analisis regresi sederhana menunjukkan koefisien korelasi rxy = .483 dengan p = .000 (p<0,05). Hasil tersebut menunjukkan bahwa hipotesis diterima, yaitu terdapat hubungan positif antara pengungkapan diri melalui media sosial WhatsApp dengan komunikasi interpersonal siswa semester empat SMA Negeri 1 Salatiga dengan sumbangan efektif sebesar 23,4% sedangkan 76,6% sisanya dipengaruhi oleh faktor lain yang tidak diungkap dalam penelitian ini

Generation of mesenchymal stromal cells from cord blood: evaluation of in vitro quality parameters prior to clinical use

Dexamethasone scheduling in MNC culture. The supplement was added in standard medium until the detection of MSC colonies (n = 16 CB units) or alternatively added for the first week of MNC culture only (n = 34). (DOCX 120 kb

In vitro analysis of Quantum Molecular Resonance effects on human mesenchymal stromal cells

Effects of high frequency electromagnetic fields and electric currents on biological systems, in particular concerning stem cells, are not extensively studied. Medical devices based on Quantum Molecular Resonance (QMR) technology are actually used in clinical practice for the treatment of musculoskeletal disorders and post-surgical articulation conditions. QMR is a new technology based on the quantum theory and assumes that a quantum value of energy exists for breaking every type of molecular bond without any increase of temperature. QMR produces waves at high frequencies (4-64 MHz) and low intensity through oscillating electric currents. This work aimed at understanding how QMR acts on the regenerative capacities of human bone marrow mesenchymal stromal cells (MSC). MSC are multipotent non-hematopoietic cells with peculiar immunomodulatory and angiogenic properties and a supportive role in hematopoiesis. Moreover their capacity to be recruited in damaged tissues and to differentiate in tissues of mesodermal origin, make them suitable for cellular therapy and in regenerative medicine. MSC cultures were exposed to QMR for two cycles of treatment at two different nominal powers (40 and 80) using an experimental medical device supplied and patented by Telea Electronic Engineering S.r.l. (Italy). QMR treatments maintained MSC identity and function in terms of morphology, phenotype and multilineage differentiation (adipogenesis, osteogenesis and chondrogenesis). Moreover the treatment did not affect cell viability or proliferation and preserved their intrinsic migration capacity. Microarray analysis revealed that QMR stimulation at 40 nominal power was likely more effective than 80 in inducing molecular changes, as demonstrated by the greater number of up- and down-regulated genes. Specifically, it was observed that genes modulated at 40 were involved into cellular and tissue regeneration processes like extracellular matrix (ECM) remodeling, angiogenesis, cellular migration and regulation of actin filaments. In this regard, quantitative real time PCR results confirmed expression of MMP1, PLAT and A2M genes. These genes generate transcripts for secreted proteins and are involved in ECM remodeling through the fibrinolytic system, which is also implicated in embryogenesis, wound healing and angiogenesis. We conclude that QMR stimulation might favor tissue regeneration probably supporting neoangiogenesis. Further studies are needed to evaluate how these proteins are implicated in MSC regenerative response after QMR exposition.Gli effetti dei campi elettromagnetici e delle correnti elettriche ad alta frequenza sui sistemi biologici, in particolare nei confronti delle cellule staminali, non sono stati ancora studiati in modo approfondito. Strumentazioni mediche che si basano sulla tecnologia della Risonanza Quantica Molecolare (QMR) sono attualmente utilizzate nella pratica clinica per trattare patologie muscolo-scheletriche e traumi post-chirurgici alle articolazioni. La QMR è una nuova tecnologia che si basa sulla teoria dei quanti ed assume che esiste un valore quantico di energia capace di rompere ogni tipo di legame molecolare senza produrre un incremento di temperatura. La QMR genera onde ad alta frequenza (4-64 MHz) e a basse intensità mediante correnti elettriche oscillanti. Questo lavoro è focalizzato nella comprensione dei meccanismi alla base dell’azione della QMR sulle capacità rigenerative di cellule mesenchimali stromali (MSC) umane ottenute da midollo osseo. Quest’ultime sono cellule multipotenti non ematopoietiche con peculiari proprietà immunomodulanti e di supporto all’ematopoiesi ed alla neoangiogenesi. In virtù della proprietà di queste cellule di essere reclutate in presenza di un danno tissutale, esse trovano applicazione in protocolli di terapia cellulare e medicina rigenerativa. Le colture di MSC sono state esposte a due cicli di trattamento con QMR applicando due diversi settaggi noti come potenze nominali 40 e 80, mediante l’uso di uno strumento medico sperimentale fornito e patentato dalla Telea Electronic Engineering S.r.l. (Italia). In questo studio abbiamo dimostrato che il trattamento con QMR conserva l’identità e la funzione delle MSC in termini di morfologia, fenotipo e capacità di differenziare in tessuto adiposo, osseo e cartilagineo. Inoltre la stimolazione non altera la vitalità o la proliferazione delle cellule e mantiene la loro intrinseca capacità migratoria. L’analisi mediante microarray ha suggerito una maggiore efficacia della stimolazione alla potenza nominale 40 nell’indurre cambiamenti a livello molecolare, come dimostrato dal maggior numero di geni up- e down- regolati. In modo specifico, è stato osservato che i geni modulati con il settaggio 40 sono coinvolti nei processi di rimodellamento della matrice extracellulare, angiogenesi, migrazione cellulare e regolazione dei filamenti actinici. Infine risultati ottenuti in real time PCR quantitativa hanno confermato l’espressione dei geni MMP1, PLAT e A2M. Questi geni producono trascritti per proteine secrete e sono coinvolti nel rimodellamento della matrice extracellulare attraverso il sistema fibrinolitico, il quale è implicato nell’embriogenesi, nella guarigione delle ferite e nell’angiogenesi. In conclusione, la stimolazione con QMR potrebbe favorire la rigenerazione tissutale coinvolgendo probabilmente vie di segnale implicate nella neoangiogenesi. Successivi studi saranno necessari per valutare in modo approfondito come queste proteine possano essere implicate nella risposta rigenerativa delle MSC dopo esposizione con QMR

samples_dendrogram.pdf

Cluster dendrogram of samples according by similarity of gene expression profile<br

Platelet lysate as a substitute for animal serum for the ex-vivo expansion of mesenchymal stem/stromal cells: present and future

The use of fetal bovine serum (FBS) as a cell culture supplement is discouraged by regulatory authorities to limit the risk of zoonoses and xenogeneic immune reactions in the transplanted host. Additionally, FBS production came under scrutiny due to animal welfare concerns. Platelet derivatives have been proposed as FBS substitutes for the ex-vivo expansion of mesenchymal stem/stromal cells (MSCs) since platelet-derived growth factors can promote MSC ex-vivo expansion. Platelet-derived growth factors are present in platelet lysate (PL) obtained after repeated freezing-thawing cycles of the platelet-rich plasma or by applying physiological stimuli such as thrombin or CaCl2.PL-expanded MSCs have been used already in the clinic, taking advantage of their faster proliferation compared with FBS-expanded preparations. Should PL be applied to other biopharmaceutical products, its demand is likely to increase dramatically. The use of fresh platelet units for the production of PL raises concerns due to limited availability of platelet donors. Expired units might represent an alternative, but further data are needed to define safety, including pathogen reduction, and functionality of the obtained PL. In addition, relevant questions concerning the definition of PL release criteria, including concentration ranges of specific growth factors in PL batches for various clinical indications, also need to be addressed. We are still far from a common definition of PL and standardized PL manufacture due to our limited knowledge of the mechanisms that mediate PL-promoting cell growth. Here, we concisely discuss aspects of PL as MSC culture supplement as a preliminary step towards an agreed definition of the required characteristics of PL for the requirements of manufacturers and users

The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro

Abstract Background The use of fetal bovine serum (FBS) as a media supplement for the ex vivo expansion of bone-marrow derived mesenchymal stromal cells (BM-MSC) has been discouraged by regulatory agencies, due to the risk of transmitting zoonoses and to elicit immune reactions in the host once transplanted. Platelet derivatives are valid FBS substitutes due to their content of growth factors that can be released disrupting the platelets by physical methods or physiological stimuli. We compared platelet derivatives produced by freezing/thawing (platelet lysates, PL) or after CaCl2 activation (platelet releasate surnatant rich in growth factors, PR-SRGF) for their content in growth factors and their ability to support the ex vivo expansion of BM-MSC. Methods The cytokine content in the two platelet derivatives was evaluated. BM-MSC were expanded in complete medium containing 10, 7.5 and 5% PL or PR-SRGF and the cell phenotype, clonogenic capacity, immunomodulation properties and tri-lineage differentiation potential of the expanded cells in both media were investigated. Results The concentration of PDGF-AB, PDGF-AA, PDGF-BB in PR-SRGF resulted to be respectively 5.7×, 1.7× and 2.3× higher compared to PL. PR-SRGF promoted a higher BM-MSC proliferation rate compared to PL not altering BM-MSC phenotype. Colony forming efficiency of BM-MSC expanded in PR-SRGF showed a frequency of colonies significantly higher than cells expanded in PL. BM-MSC expanded in PL or PR-SRGF maintained their immunomodulatory properties against activated lymphocytes even if BM-MSC expanded in FBS performed significantly better. Conclusions The method used to release platelet factors significantly affects the enrichment in growth factors and overall product performance. The standardization of the production process of platelet derivatives and the definition of their release criteria requires further investigation

Additional file 2: Table S1. of High-throughput immunophenotypic characterization of bone marrow- and cord blood-derived mesenchymal stromal cells reveals common and differentially expressed markers: identification of angiotensin-converting enzyme (CD143) as a marker differentially expressed between adult and perinatal tissue sources

Mean and CV values of log2 FI for each of 246 investigated markers. (DOCX 22 kb

Asymmetrical high-flow nasal cannula performs similarly to standard interface in patients with acute hypoxemic post-extubation respiratory failure: a pilot study.

BACKGROUND: Standard high-flow nasal cannula (HFNC) is a respiratory support device widely used to manage post-extubation hypoxemic acute respiratory failure (hARF) due to greater comfort, oxygenation, alveolar recruitment, humidification, and reduction of dead space, as compared to conventional oxygen therapy. On the contrary, the effects of the new asymmetrical HFNC interface (Optiflow® Duet system (Fisher &amp; Paykel, Healthcare, Auckland, New Zealand) is still under discussion. Our aim is investigating whether the use of asymmetrical HFNC interface presents any relevant difference, compared with the standard configuration, on lung aeration (as assessed by end-expiratory lung impedance (EELI) measured by electrical impedance tomography (EIT)), diaphragm ultrasound thickening fraction (TFdi) and excursion (DE), ventilatory efficiency (estimated by corrected minute ventilation (MV)), gas exchange, dyspnea, and comfort. METHODS: Pilot physiological crossover randomized controlled study enrolling 20 adults admitted to the Intensive Care unit, invasively ventilated for at least 24&nbsp;h, and developing post-extubation hARF, i.e., PaO2/set FiO2 &lt; 300 mmHg during Venturi mask (VM) within 120&nbsp;min after extubation. Each HFNC configuration was applied in a randomized 60&nbsp;min sequence at a flow rate of 60&nbsp;L/min. RESULTS: Global EELI, TFdi, DE, ventilatory efficiency, gas exchange and dyspnea were not significantly different, while comfort was greater during asymmetrical HFNC support, as compared to standard interface (10 [7-10] and 8 [7-9], p-value 0.044). CONCLUSIONS: In post-extubation hARF, the use of the asymmetrical HFNC, as compared to standard HFNC interface, slightly improved patient comfort without affecting lung aeration, diaphragm activity, ventilatory efficiency, dyspnea and gas exchange. CLINICAL TRIAL NUMBER: ClinicalTrial.gov. REGISTRATION NUMBER: NCT05838326 (01/05/2023). NEW &amp; NOTEWORTHY: The asymmetrical high-flow nasal cannula oxygen therapy (Optiflow® Duet system (Fisher &amp; Paykel, Healthcare, Auckland, New Zealand) provides greater comfort as compared to standard interface; while their performance in term of lung aeration, diaphragm activity, ventilatory efficiency, dyspnea, and gas exchange is similar

Selected genes for testing DNA microarray outcomes in quantitative real-time PCR.

<p>Selected genes for testing DNA microarray outcomes in quantitative real-time PCR.</p

<i>In-vitro</i> analysis of Quantum Molecular Resonance effects on human mesenchymal stromal cells

<div><p>Electromagnetic fields play an essential role in cellular functions interfering with cellular pathways and tissue physiology. In this context, Quantum Molecular Resonance (QMR) produces waves with a specific form at high-frequencies (4–64 MHz) and low intensity through electric fields. We evaluated the effects of QMR stimulation on bone marrow derived mesenchymal stromal cells (MSC). MSC were treated with QMR for 10 minutes for 4 consecutive days for 2 weeks at different nominal powers. Cell morphology, phenotype, multilineage differentiation, viability and proliferation were investigated. QMR effects were further investigated by cDNA microarray validated by real-time PCR. After 1 and 2 weeks of QMR treatment morphology, phenotype and multilineage differentiation were maintained and no alteration of cellular viability and proliferation were observed between treated MSC samples and controls. cDNA microarray analysis evidenced more transcriptional changes on cells treated at 40 nominal power than 80 ones. The main enrichment lists belonged to development processes, regulation of phosphorylation, regulation of cellular pathways including metabolism, kinase activity and cellular organization. Real-time PCR confirmed significant increased expression of MMP1, PLAT and ARHGAP22 genes while A2M gene showed decreased expression in treated cells compared to controls. Interestingly, differentially regulated MMP1, PLAT and A2M genes are involved in the extracellular matrix (ECM) remodelling through the fibrinolytic system that is also implicated in embryogenesis, wound healing and angiogenesis. In our model QMR-treated MSC maintained unaltered cell phenotype, viability, proliferation and the ability to differentiate into bone, cartilage and adipose tissue. Microarray analysis may suggest an involvement of QMR treatment in angiogenesis and in tissue regeneration probably through ECM remodelling.</p></div