Membrane techniques for removal detergents and petroleum products from carwash effluents: a review

One of the most significant urban services is the carwash, which generates large amounts of wastewater containing a variety of pollutants, including sand, gravel, suspended solids, surfactants, oil products, diesel cleaners, etc., that may cause environmental pollution when transferred to the sewage system without any treatment. The effective treatment is crucial to prevent environmental pollution as well as to recycle the water source. Contaminants are removed from carwash effluent using a variety of treatment technologies. This review focuses on identifying and comparing efficiency of using advanced commercial and modified membrane filtration techniques, meeting discharge standard regulations, to treat carwash impurities, especially detergents/surfactants (anionic surfactant) and petroleum products (oil/grease). The results of this review indicate that ultrafiltration membrane (UF) is the most common membrane filtration technology for carwash wastewater treatment. Additionally, the adoption of traditional pre-treatment processes may be advantageous before utilization of membrane process for treating carwash wastewater; although conventional treatment processes can produce a high quality of effluent, they are less effective than membrane systems

Problems of labor market of modern Russia in conditions of stable economic growth

The article comprises a set of theoretical and methodological statements about the development of modern labor market. The author analyzes potential of socio-economic relations in Russia, reveals its weaknesses. Russian labor market is largely hinders its development due to a mismatch of the most important elements - the price of labor, consumer-level of population with the cost of labor

Analysis of PR-text: The semantic aspect

PR-text is a complex, from the semantic point of view, formation, due to its dual nature. The PR-text is presented in two semantic guises - as the meaning, specified by social and professional traditions of oral culture, and as the sense, defined by the author of the text. One of the main properties of the text is the ability to transmit and contain information. The text is a complex formation, incorporating, at the same time existing in its structure, linguistic, logic, speech, cognitive, stylistic, expressive and other subsystems. For a deeper understanding of the semantics of the PR text, it is necessary to define the semantic field of the text, its content, how the elements of the language system and the formal properties of the PR-text interact

DIAGNOSTICS AND TREATMENT OF LOW BACK PAIN

The paper gives an update on the principles of diagnosis and medical and surgical treatments in patients with low back pain (LBP), by taking into account the clinical phenomenology of pain manifestations. It considers the use of nonsteroidal anti-inflammatory drugs, myorelaxants, anticonvulsants, antidepressants, and other agents. The data of clinical trials of meloxicam used in LBP are presented. The currently available surgical treatments for LBP are described

Effect of the regulation system of metabolic nitrogen exchange on biosynthesis of serine proteinases from Bacillus intermedius

The regulatory link between biosynthesis of Bacillus intermedius subtilisin-like serine proteinase and nitrogen metabolism in B. intermedius cells was determined. The level of the enzyme biosynthesis by the recombinant strain of Bacillus subtilis in the medium containing ammonium ions was three- to fivefold less than that in the medium with poorly utilized sodium nitrate. Accumulation of glutamyl endopeptidase in a culture liquid of this microorganism did not depend on the source of nitrogen present in the medium. During cultivation in the rich medium, the productivity of subtilisin-like proteinase in the recombinant B. subtilis strain carrying a mutation in the NrgB sensor protein was demonstrated to increase threefold compared to that of the control strain. In the minimal culture medium, mutation in the nrgB gene abolished the effect of a nitrogen source on the level of the subtilisin-like proteinase gene expression. At the same time, this mutation did not affect glutamyl endopeptidase biosynthesis. Thus, expression of the gene coding for subtilisin-like proteinase from B. intermedius is suggested to be positively regulated by the regulatory system of nitrogen metabolism. © Pleiades Publishing, Ltd., 2009

STEAM-Education as Innovative Technology for Industry 4.0

The article is devoted to the development of STEAM-education design model, which is based on project training in the so-called “creative spaces.” “Creative spaces” are integration platforms for schoolchildren, students and postgraduates working in the format of joint work on projects initiated by various structures of society and business. The relevance of the study is due to the fact that among the skills necessary for successful professional activity in the era of the digital industry, according to experts, the ability to artistic creativity takes a special place. The analysis of the experience of various countries in the implementation of STEM and STEAM-education is made and effective ways of structuring technical disciplines, art and creative activity in a single integration program are identified. The article presents the results of experimental work on determining the level of formation of such competencies as the ability to manage projects and processes, system thinking, the ability to artistic creativity, the ability to work with teams, groups and individuals, the ability to work in a mode of high uncertainty and rapid change of conditions of tasks. It is shown that the use of “creative spaces” for the implementation of project activities of schoolchildren and students, the inclusion of the category “art” in its content allows students to form skills and competencies necessary for industry 4.0, that is, the proposed model can be considered as a universal tool for high-quality training of schoolchildren and students for professional activities in modern conditions

The novel adams-like microbial metalloendopeptidase

Heterologous gene expression of the extracellular minor metalloendopeptidase of Bacillus pumilus 3-19 (AN EU678894) in the protease-deficient B. pumilus 3-19 strain has been studied. The enzyme fraction in the total pool of B. pumilus 3-19 proteases is less than 8%. The enzyme was isolated from the culture liquid of the recombinant strain and purified to a homogeneous state; its primary structure was determined and its physicochemical properties were investigated. The secreted metalloendopeptidase of B. pumilus 3-19 is the first prokaryotic homologue of the eukaryotic adamalysin/reprolysin protein family.© Pleiades Publishing, Ltd., 2012

Features of gene expression of Bacillus pumilus metalloendopeptidase

© 2016, Pleiades Publishing, Ltd.Features of gene expression of the secreted Bacillus pumilus metalloendopeptidase belonging to the adamalysin/reprolysin family were investigated. In the regulatory region of the gene, we identified hypothetical binding sites for transcription factors CcpA and TnrA. We found that the expression of the metalloendopeptidase gene is controlled by mechanisms of carbon and nitrogen catabolite repression. In experiments involving nitrogen metabolism regulatory protein mutant strains, we found that the control of the metalloendopeptidase gene expression involves proteins of ammonium transport GlnK and AmtB interacting with the TnrA-regulator

Exosomal release of L-plastin by breast cancer cells facilitates metastatic bone osteolysis

Bone metastasis from breast and prostate carcinomas is facilitated by activation of bone-resorbing osteoclasts. Using proteomics approaches, we have identified peroxiredoxin-4 (PRDX4) as a cancer-secreted mediator of osteoclastogenesis. We now report characterization of L-plastin in the conditioned media (CM) of MDA-MB-231 human breast cancer cells using immunoblotting and mass spectrometry. The osteoclastogenic potential of MDAMB-231 CM with siRNA-silenced L-plastin was significantly reduced. L-plastin was detected in cancer-derived exosomes, and inhibition of exosomal release significantly decreased the osteoclastogenic capacity of MDA-MB-231 CM. When added to osteoclast precursors primed with RANKL for 2 days, recombinant L-plastin induced calcium/NFATc1-mediated osteoclastogenesis to the levels similar to continuous treatment with RANKL. Using shRNA, we generated MDA-MB-231 cells lacking L-plastin, PRDX4, or both and injected these cell populations intratibially in CD-1 immunodeficient mice. Micro-CT and histomorphometric analysis demonstrated a complete loss of osteolysis when MDA-MB-231 cells lacking both L-plastin and PRDX4 were injected. A meta-analysis established an increase in L-plastin and PRDX4 mRNA expression in numerous human cancers, including breast and prostate carcinomas. This study demonstrates that secreted L-plastin and PRDX4 mediate osteoclast activation by human breast cancer cells

Analysis of intensive therapy for massive blood loss in obstetrics

This article examines the volume and composition of infusion therapy for massive blood loss (more than 1500 ml) based on the principle of "control of resuscitation" by 42 patients in the Regional perinatal center of Yekaterinburg. The effectiveness of restrictive infusion therapy for massive blood loss in obstetrics have been shown.В настоящей статье произведено исследование объема и состава инфузионной терапии массивной кровопотери (более 1500 мл) на основе принципа «контроля за реанимацией» у 42 пациенток в Областном перинатальном центре г. Екатеринбурга. Показана эффективность рестриктивной инфузионной терапии при массивной кровопотере в акушерстве