Figure S2. Mislocalized Sdt variants do not displace endogenous PATJ or PAR-6

(A-D) Sdt-GFP was ubiquitous expressed in wild type embryos and distribution of GFP, PATJ and PAR-6 was analyzed in stage 11-12 embryos. Scale bars = 5µm

Figure S1 Expression of Sdt in embryogenesis.

(A) Extracts of 0-16h old embryos of either sdtK85 germ lines clones (mated with males carrying an FM7-ChFP balancer and sorted against ChFP) or wild type flies were blotted against Sdt and Actin. Both bands seen above the 100kDa marker band appear to be specific. (B) Extracts of staged embryos were blotted against Sdt and Actin. (C-D) Heterozygous and zygotic mutant sdtK85 embryos were stained with anti Sdt and DAPI. Note that the morphology of sdtK85 mutant embryos is already disturbed. Scale bars = 50µm