(A) Extracts of 0-16h old embryos of either sdtK85 germ lines clones (mated with males carrying an FM7-ChFP balancer and sorted against ChFP) or wild type flies were blotted against Sdt and Actin. Both bands seen above the 100kDa marker band appear to be specific. (B) Extracts of staged embryos were blotted against Sdt and Actin. (C-D) Heterozygous and zygotic mutant sdtK85 embryos were stained with anti Sdt and DAPI. Note that the morphology of sdtK85 mutant embryos is already disturbed. Scale bars = 50µm
