PENINGKATAN MUTU GENETIK INDUK DAN CALON INDUK KAMBING PE PROLIFIK MELALUI PEMANFAATAN PAKAN KULIT BUAH KAKAO

Pod husks from waste cocoa crops used as alternative feed goats Peranakan Etawah. This study aims to determine the impact of the pod husks as a feed supplement to increase body size of adult female and parent in preparing for pregnancy. A total of 30 goats were divided into 16 tails dara and 14 breeding, reared intensively for 60 days. Pod husks were feeding as many as 1000-1500 g / head / day. The analysis used the PCR RFLP. The results showed the average growth of Body Lenght for the adult female, the prolific parent and single parent respectively by 1.75, 2:18 and 1:59 cm, while the average increase respectively Shoulder Height 1:49, and 1:12 1.97 cm. The results of the analysis of PCR RFLP GH MspI produced three genotypes, namely TT; TC and CC. Obtained genotype frequencies of TT (0091); TC (0409) and CC (0500). GH MspI gene polymorphism is not associated with the prolific nature, but related with the average increase in body size. It can be seen in the TT genotype showed the average increase Body Lenght (2.26 cm) and Shoulder Height (1.80), while TC and CC is almost the same (not different). Program selection on the growth potential of goats can use TT genotype

PENGARUH PENAMBAHAN EKSTRAK BUAH MENGKUDU (Morinda citrifolia Linn) DALAM PENGENCER TERHADAP MOTILITAS SPERMATOZOA PADA SEMEN CAIR SAPI BALI

The processing of liquid cement is likely to decrease sperm motility. It can be caused by the oxidative reaction to increase the amount of free radicals. Noni (Morinda citrifolia Linn) contains many antioxidants that can serve counteract free radicals. This study aims to determine the addition of extracts of noni (Morinda citrifolia Linn) in order to maintain the individual motility of spermatozoa in the semen liquid Bali cattle. This research was conducted at the Laboratory of Animal Reproduction Semen Processing Unit Faculty of Animal Husbandry Hasanuddin University Makassar. Extracts of noni fruit (Morinda citrifolia Linn) effect on sperm motility (P <0.05). Storage P0 at 3 h to day 2 showed higher motility. Concentration 0,02 gr/ml (P1) gives the best results in maintaining sperm motility on day 3rd to 5th. Extracts of noni fruit (Morinda citrifolia Linn) can retain sperm motility because many contain many antioxidants that serve to neutralize free radicals cause a decrease in sperm motility during equilibration at 5 0C

Caprin-1, a novel Cyr61-interacting protein, promotes osteosarcoma tumor growth and lung metastasis in mice

Osteosarcoma (OS) is the most common primary bone malignancy in children and adolescents. More than 30% of patients develop lung metastasis, which is the leading cause of mortality. Recently, the extracellular matrix protein Cyr61 has been recognized as a malignancy promoting protein in OS mouse model with prognostic potential in human OS. In this study, we aimed at the identification of novel Cyr61-interacting proteins. Here we report that Cyr61 associates with Caprin-1, and confocal microscopy showed that stable ectopic expression of Caprin-1 leads to the formation of stress granules containing Caprin-1 and Cyr61, confers resistance to cisplatin-induced apoptosis, and resulted in constitutive phosphorylation of Akt and ERK1/2. Importantly, ectopic expression of Caprin-1 dramatically enhanced primary tumor growth, remarkably increased lung metastatic load in a SCID intratibial OS mouse model, and decreased significantly (p<0.0018) the survival of the mice. Although Caprin-1 expression, evaluated with a tissue microarray including samples from 59 OS patients, failed to be an independent predictor for the patients' outcome in this limited cohort of patients, increased Caprin-1 expression indicated a tendency to shortened overall survival, and more strikingly, Cyr61/Caprin-1 co-expression was associated with worse survival than that observed for patients with tumors expressing either Cyr61 or Caprin-1 alone or none of these proteins. The findings imply that Caprin-1 may have a metastasis promoting role in OS and show that through resistance to apoptosis and via the activation of Akt and ERK1/2 pathways, Caprin-1 is significantly involved in the development of OS metastasis

Identification of Distinctive Patterns of USP19-Mediated Growth Regulation in Normal and Malignant Cells

We previously reported that the USP19 deubiquitinating enzyme positively regulates proliferation in fibroblasts by stabilizing KPC1, a ubiquitin ligase for p27Kip1. To explore whether this role of USP19 extends to other cellular systems, we tested the effects of silencing of USP19 in several human prostate and breast models, including carcinoma cell lines. Depletion of USP19 inhibited proliferation in prostate cancer DU145, PC-3 and 22RV1 cells, which was similar to the pattern established in fibroblasts in that it was due to decreased progression from G1 to S phase and associated with a stabilization of the cyclin-dependent kinase inhibitor p27Kip1. However, in contrast to previous findings in fibroblasts, the stabilization of p27Kip1 upon USP19 depletion was not associated with changes in the levels of the KPC1 ligase. USP19 could also regulate the growth of immortalized MCF10A breast epithelial cells through a similar mechanism. This regulatory pattern was lost, though, in breast cancer MCF7 and MDA-MB-231 cells and in prostate carcinoma LNCaP cells. Of interest, the transformation of fibroblasts through overexpression of an oncogenic form of Ras disrupted the USP19-mediated regulation of cell growth and of levels of p27Kip1 and KPC1. Thus, the cell context appears determinant for the ability of USP19 to regulate cell proliferation and p27Kip1 levels. This may occur through both KPC1 dependent and independent mechanisms. Moreover, a complete loss of USP19 function on cell growth may arise as a result of oncogenic transformation of cells

Induction of p38- and gC1qR-dependent IL-8 expression in pulmonary fibroblasts by soluble hepatitis C core protein

BACKGROUND: Recent studies suggest that HCV infection is associated with progressive declines in pulmonary function in patients with underlying pulmonary diseases such as asthma and chronic obstructive pulmonary disease. Few molecular studies have addressed the inflammatory aspects of HCV-associated pulmonary disease. Because IL-8 plays a fundamental role in reactive airway diseases, we examined IL-8 signaling in normal human lung fibroblasts (NHLF) in response to the HCV nucleocapsid core protein, a viral antigen shown to modulate intracellular signaling pathways involved in cell proliferation, apoptosis and inflammation. METHODS: NHLF were treated with HCV core protein and assayed for IL-8 expression, phosphorylation of the p38 MAPK pathway, and for the effect of p38 inhibition. RESULTS: Our studies demonstrate that soluble HCV core protein induces significant increases in both IL-8 mRNA and protein expression in a dose- and time-dependent manner. Treatment with HCV core led to phosphorylation of p38 MAPK, and expression of IL-8 was dependent upon p38 activation. Using TNFα as a co-stimulant, we observed additive increases in IL-8 expression. HCV core-mediated expression of IL-8 was inhibited by blocking gC1qR, a known receptor for soluble HCV core linked to MAPK signaling. CONCLUSION: These studies suggest that HCV core protein can lead to enhanced p38- and gC1qR-dependent IL-8 expression. Such a pro-inflammatory role may contribute to the progressive deterioration in pulmonary function recently recognized in individuals chronically infected with HCV

Caractérisation et physiopathologie moléculaires des micro métastases

COMPIEGNE-BU (601592101) / SudocSudocFranceF

Enabling Organizational Resilience Amidst Disasters: Experiences of Work Organizations in Southeast Asia

This chapter describes the environmental risks and vulnerabilities faced by work organizations in Southeast Asia. It also presents cases that demonstrate how these organizations respond to disasters and natural hazards. To situate the case discussions, a review of existing studies of organizational resilience, particularly those that propose definitions, models, and frameworks is presented. The cases from the Philippines and Thailand illustrate how active and integrative efforts at building resilience can be institutionalized at the organizational level