Capsule independent uptake of the fungal pathogen Cryptococcus neoformans into brain microvascular endothelial cells.

Cryptococcosis is a life-threatening fungal disease with a high rate of mortality among HIV/AIDS patients across the world. The ability to penetrate the blood-brain barrier (BBB) is central to the pathogenesis of cryptococcosis, but the way in which this occurs remains unclear. Here we use both mouse and human brain derived endothelial cells (bEnd3 and hCMEC/D3) to accurately quantify fungal uptake and survival within brain endothelial cells. Our data indicate that the adherence and internalisation of cryptococci by brain microvascular endothelial cells is an infrequent event involving small numbers of cryptococcal yeast cells. Interestingly, this process requires neither active signalling from the fungus nor the presence of the fungal capsule. Thus entry into brain microvascular endothelial cells is most likely a passive event that occurs following ‘trapping’ within capillary beds of the BBB

Fighting antimicrobial resistance : who should lead the agenda?

The TWENDE consortium (https://infection.st-andrews.ac.uk/twende-overview/) is funded by the European and Developing Countries Clinical Trials Partnership (EDCTP).Publisher PDFPeer reviewe

Cattle Production From Native Pastures in the Semi-Humid Grasslands of Uganda

The cattle population of Uganda, estimated at 6 million, consists of more than 95% indigenous stock, raised on the semi-arid and semi-humid grasslands that make up 48% of the total land area and which supply over 85% of the marketed milk and meat. Native grassland pastures, which vary seasonal in quantity and quality due to rainfall and temperature variations, are the sole feed resource for cattle (Mbuza et al., 1992). This study examined the effects of seasonal herbage mass (HM), dietary crude protein (CPd), detergent lignin (ADLd) and digestibility (DiG) on body condition scores of milking cows grazed only on natural grassland pastures

Mycobacterial load assay

Tuberculosis is a difficult disease to treat, a process made more harder as tools to monitor treatment response only provide a result long after the patient has provided a sample. The mycobacterial load assay (MBLA) provides a simple molecular test to quantify and determine the viability of M. tuberculosis in human or other samples.Postprin

Ensiling Characteristics and Nutritive Value of Browse/Maize Forage Mixtures

The practice of growing fodder tree and shrubs is being advocated for and adopted in smallholder dairy production systems. In Uganda, Calliandra calothyrsus, Gliricidia sepium, and Leucaena leucocephala have been identified and recommended as the most suitable species (Sabiiti, 2001). However tree foliage contains toxic compounds (Lowry, 1990), which may be alleviated by ensiling. The objective of the experiment was to study the ensiling characteristics of browse/ maize forage mixtures and their nutritive value when fed to lactating dairy cows

Use of Forage Legumes to Restore Overgrazed Natural Grasslands in Uganda

The natural grasslands of Uganda support over 95 % of the country\u27s livestock. They are also used by wildlife and protect soil resources from heat and erosion. Unfortunately, the pastoral/agro-pastoral communities which derive their livelihoods from these grasslands have in turn degraded them through overgrazing and uncontrolled burning, thus lowering their productivity (pasture and animal production) and biodiversity. The natural grasses (e.g. Panicum maximum, Brachiaria brizantha, Setaria anceps, Themeda triandra) mature rapidly and lose quality. Furthermore, the indigenous legumes (e.g. Neonotonia wightii, Desmodium adcsendens, Indigofera errecta) are less persistent and productive to maintain feed quality and hence animal production. There is a need to introduce into the grassland ecosystem alternative forage legumes that combine both persistence and productivity

Experimental Models of Cryptococcosis

Cryptococcosis is a life-threatening fungal disease that infects around one million people each year. Establishment and progression of disease involves a complex interplay between the fungus and a diverse range of host cell types. Over recent years, numerous cellular, tissue, and animal models have been exploited to probe this host-pathogen interaction. Here we review the range of experimental models that are available for cryptococcosis research and compare the relative advantages and limitations of the different systems