EVALUATION OF CYTOTOXIC ACTIVITY OF ETHYL ACETATE EXTRACT OF PIGMENT FROM PSEUDOMONAS AERUGINOSA

Objective: Bacterial pigments have promising applications in food, cosmetics, textile, and therapeutics. Pigments from microbial origin are stable, safer, cost effective, easy production, and extraction and thus preferred over other natural sources. Under this backdrop, isolation and characterization of pigment-producing bacteria and analysis of bioactivity of the pigment were the aim of the study. From the literature studies, the pigment production was found to be influenced by various physical factors which directed the study toward optimization of physical parameters for pigment production. Methods: Isolation of pigment-producing bacteria from water sample, cultural, and microscopic identification was done as per the standard protocol. Extraction of pigment by solvent extraction was carried out and its antibacterial and cytotoxic activity was assayed. Results: Molecular characterization of the bacteria resembled the query sequence of the isolate to 99% with Pseudomonas aeruginosa strain. Extraction of pigment by solvent extraction method resulted in crude pigment extract with antibacterial activity against Gram-negative bacteria (17 mm zone of inhibition) at 100 μg/ml concentration. Pigment showed dose-dependent inhibition on proliferation of HeLa cells at the concentration of 345.83 μg/mL. Conclusion: From the above results, it was evident that the pigment extracted from the bacterial isolate Pseudomonas aeruginosa strain JBT18N was therapeutically potential

Real or Artificial? Intergroup Biases in Mind Perception in a Cross-Cultural Perspective

Recent research suggests that attributions of aliveness and mental capacities to faces are influenced by social group membership. In this article, we investigated group related biases in mind perception in participants from a Western and Eastern culture, employing faces of varying ethnic groups. In Experiment 1, Caucasian faces that ranged on a continuum from real to artificial were evaluated by participants in the UK (in-group) and in India (out-group) on animacy, abilities to plan and to feel pain, and having a mind. Human features were found to be assigned to a greater extent to faces when these belonged to in-group members, whereas out-group faces had to appear more realistic in order to be perceived as human. When participants in India evaluated South Asian (in-group) and Caucasian (out-group) faces in Experiment 2, the results closely mirrored those of the first experiment. For both studies, ratings of out-group faces were significantly predicted by participants’ levels of ethnocultural empathy. The findings highlight the role of intergroup processes (i.e., in-group favoritism, out-group dehumanization) in the perception of human and mental qualities and point to ethnocultural empathy as an important factor in responses to out-groups

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Not AvailableBackground In this study, whole genome re-sequencing of rust resistant soybean genotype EC241780 was performed to understand the genomic landscape involved in the resistance mechanism. Methods A total of 374 million raw reads were obtained with paired-end sequencing performed with Illumina HiSeq 2500 instrument, out of which 287.3 million high quality reads were mapped to Williams 82 reference genome. Comparative sequence analysis of EC241780 with rust susceptible cultivars Williams 82 and JS 335 was performed to identify sequence variation and to prioritise the candidate genes. Results Comparative analysis indicates that genotype EC241780 has high sequence similarity with rust resistant genotype PI 200492 and the resistance in EC241780 is conferred by the Rpp1 locus. Based on the sequence variations and functional annotations, three genes Glyma18G51715, Glyma18G51741 and Glyma18G51765 encoding for NBS-LRR family protein were identified as the most prominent candidate for Rpp1 locus. Conclusion The study provides insights of genome-wide sequence variation more particularly at Rpp1 loci which will help to develop rust resistant soybean cultivars through efficient exploration of the genomic resource.Not Availabl

Peoples’ Views About the Acceptability of Executive Bonuses and Compensation Policies

International audienceWe applied a technique borrowed from the field of bioethics to test whether justice-related factors influence laypersons’ decisions concerning business ethics. In the first experiment, participants judged the acceptability of remuneration policies and in the second that of executive bonuses. In each study, participants judged a set of 36 situations. To create the scenarios, we varied (a) retributive justice—the amount of remuneration; (b) procedural justice—the clarity of the procedure that determined the remuneration; (c) distributive justice—the extent of the distribution of bonus payments amongst employees; and (d) restorative justice—a special compensation for hazardous working conditions or accidents at work. K-means clustering of all 36 judgments revealed four different personal positions in both experiments. One group of people readily accepted all situations. The other three groups’ judgments were mainly a function of distributive justice modulated in different ways by the context determined by the other variables. Furthermore, people conceive of distributive justice as categorical: Acceptability judgments only increase if companies give bonuses to all employees. Granting bonuses to a subset (i.e. mangers or executives) does not increase acceptability. Our results are useful for policy makers and provide business ethics researchers with a novel technique