Wound Healing Effectivity of Ageratum conyzoides L. Leaf Ethanolic Extract (Purple Flower Type), Centella asiatica (L.) Urban Leaf Ethanolic Extract, and Astaxanthin Combination Gel Preparation in Diabetic Animal Model

The diabetic wounds are one of the global burden diseases. In our previous study, Ageratum conyzoides L. leaf ethanolic extract (purple flower type) 10%, Centella asiatica (L.) Urban leaf ethanolic extract 5%, and astaxanthin 0.1% combination gel preparation showed remarkable wound healing effectivity. However, this wound healing effectivity in the diabetic condition is still unknown. There were three groups used, namely negative group (placebo/gel base), positive group (oxoferin/tetrachlorodecaoxide), and test group (Ageratum conyzoides L. leaf ethanolic extract (purple flower type) 10%, Centella asiatica (L.) Urban leaf ethanolic extract 5%, and astaxanthin 0.1%). Each group consisted of three male Wistar rats. We conducted diabetes induction with alloxan (175 mg/Kg BW i.p) and followed by an incision 1.5 cm on the back. All of these groups were given treatment once daily and monitored for 14 days. The results of the study showed the test group indicated significantly better effectivity than positive and negative groups (p0.05). The percentages of the wound healing effectivity of the test, positive and negative groups were 62.74%, 51.77%, and 41.65%, respectively. Ageratum conyzoides L. leaf ethanolic extract (purple flower type) 10%, Centella asiatica (L.) Urban leaf ethanolic extract 5%, and astaxanthin 0.1% combination gel preparation has excellent potential to be developed as a commercial product to treat diabetic wound conditions.

FORMULATION, CHARACTERIZATION, AND DETERMINATION OF THE DIFFUSION RATE STUDY OF ANTIOXIDANT SERUM CONTAINING ASTAXANTHIN NANOEMULSION

Objective: Astaxanthin is one of the natural carotenoids with strong antioxidant characteristics which is widely used in skin care. The aim of this study was developed to formulate and characterize the antioxidant serum containing astaxanthin nanoemulsion and the diffusion rate studies using diffusion Franz method. Methods: Astaxanthin nanoemulsion (As-NE) was prepared by using the self-nanoemulsifying method, followed by incorporation into serum preparation with the using carbomer as a gelling agent. Evaluation of serum As-NE was performed by physical, chemical characterizations and diffusion assay. Stability study was carried out in both accelerated (temperature of 40±2 °C/75±5%RH) and non-accelerated (at ambient temperature) conditions. Results: These results suggest that antioxidant serum As-NE had good physical and chemical characteristics that are suitable for topical administration. Conclusion: For the study of diffusion and stability under different storage conditions, it was proven that antioxidant serum As-NE form was packed in a carbomer as a gelling agent that could enhance the stability and diffusion rate of the astaxanthin

Sosialisasi perilaku hidup bersih dan sehat (PHBS) di lingkungan Sekolah Dasar Negeri 01 Bantar

Clean and healthy living behavior (PHBS) is a set of behaviors performed on an awareness basis, helping families, groups or communities to help themselves (independently) in the field of health  and contribute to their health. Active role in achieving the common goal, health. The majority of children walk out from their house so when they are at school they have to wash their hands, but they are used to not washing their hands while poor sanitation and hygiene can increase the risk of poisoning or infection. The purpose of this community service activity is to increase the knowledge of Bantar 01 Public Elementary School children regarding the importance of PHBS and proper hand washing. The community service activity method was by lecturing and giving questionnaires. The result of the activity is an influence on the understanding of the material received based on gender

Formulasi Dan Evaluasi Minuman Herbal Antioksidan Jahe Merah (Zingiber Officinale Rosc. Var. Rubrum)

Prevalensi kasus Covid-19 di Indonesia sangat tinggi dan salah satu faktor mortalitasnya adalah penyakit komorbid yang diakibatkan karena tingginya radikal bebas didalam tubuh. Antioksidan dari luar diperlukan sebagai asupan untuk menghambat radikal bebas. Jahe merah merupakan sumber antioksidan alami yang sering digunakan dalam campuran minuman karena memiliki rasa pedas. Penelitian ini bertujuan untuk melakukan formulasi dan mengevaluasi aktivitas antioksidan produk minuman herbal jahe merah. Tahap pertama penelitian adalah preparasi simplisia, karakterisasi simplisia, ekstraksi simplisia jahe merah dan penapisan fitokimia ekstrak. Tahap kedua adalah formulasi minuman herbal, evaluasi produk dan evaluasi antioksidan produk menggunakan metode DPPH. Komposisi minuman herbal adalah 14,23 mL sari jahe , 57 mL susu UHT, 35,7 gram gula dan 143,07 mL air. Hasil penelitian menunjukkan bahwa secara organoleptik produk stabil selama satu hari, sedangkan nilai pH 5,8; viskositas 16 cp (100 rpm); homogen dan cukup baik untuk uji hedonik. Evaluasi antikoksidan menunjukkan nilai IC50 asam askorbat dan produk minuman berturut-turut sebesar 6,39 μg/mL dan 8,44 μg/mL. Minuman herbal jahe merah memenuhi semua parameter standar dan minuman ini memiliki aktivitas antioksidan sangat kuat

Antidepressant Activity of Some Fractions of The Basil Leaves [Ocimum Basilicum (L)] on The Swiss Webster Male Mice

Objectives : Determine the antidepressant activity of some fractions of the leaves of Ocimum basilicum L Methods : The experimental methods are using Force Swimming Test and Tail Suspension Test. The experiment was divided into seven groups. Negative control group (CMC 1 %), Positive control group (fluoxetine 0,052 mg/20 g BW of mice), test I group (fraction of n-hexane 50 mg/Kg BW), test II group (fraction of ethyl acetate 50 mg/Kg BW), test III group (fraction of ethanol 50 mg/Kg BW), test IV group (combination of cyproheptadine 0,0208 mg/20 g BW of mice and fluoxetine 0,052 mg/20 g BW of mice) and test V group (combination of cyproheptadine 0,0208 mg/20 g BW of mice and fraction of ethyl acetate 50 mg/Kg BW). Result : The Force Swimming Test and Tail Suspension Test showed the same result that ethyl acetate fraction was given significant difference (p<0,05) in immobilization time reduction compared to negative control and has a better immobility reduction time than other groups. Added on cyproheptadine to fluoxetine and ethyl acetate fraction groups showed an increased in immobilization time compared to fluoxetine and ethyl acetate fraction alone. Conclusion : Ethyl acetate fraction showed better antidepressant activity than another fraction through the role of serotonin

FORMULASI HAIR TONIC EKSTRAK KECAMBAH KACANG HIJAU SEBAGAI HAIR TONIC

Tujuan penelitian ini adalah formulasi sediaan ekstrak kecambah kacang hijau (Vigna Radiata L) dan evaluasi sediaan yang berpotensi mengatasi rambut rontok (hair tonic). Ekstraksi dilakukan dengan metode maserasi menggunakan etanol 96%. Evaluasi sediaan yang dilakukan meliputi organoleptik, kerapatan, pH dan viskositas. Didapat 3 formula yaitu F1,F2 dan F3 yang memiliki karakteristik hampir sama yaitu warna kuning bening, bau khas kecambah, dan konsistensi encer. pH sediaan berturut-turut 6,14;6,45; dan 6,49 untuk F1, F2 dan F3. Viskositas sediaan ekstrak kecambah kacang hijau adalah 2,01;2,07;2,11 cP untuk F1,F2 dan F

Efektifitas Rebusan Daun Babadotan dan Kombinasinya dengan Pregabalin sebagai Anti Nyeri Neuropatik serta Uji Toksisitas Akutnya

Neuropathic pain is a condition that is difficult to treated and impaired quality of life. The objective of the study was to determine the anti-neuropathic pain effectivity of Ageratum conyzoides aqueous extract and the acute toxicity study. We had 7 groups (normal, negative, pregabalin, ageratum. conyzoides-I (AC-I) 50mg/kg BW, A. conyzoides-II (AC-II) 100mg/kg BW, combination of pregabalin and AC-II), combination of AC-II and naloxone). Each group contain 5 animals (mice). All groups was induced with pyridoxine 400 mg/kg BW i.p for 14 days to cause neuropathy and followed by hyperalgesia, allodynia, and wire-hang test. In the acute toxicity study, we had 1 group and contain 5 animals (rats).  The results of the hyperalgesia and allodynia study showed the effect of AC-II was better than AC-I. The combination of pregabalin and AC-II was better than AC-II as monotherapy (p<0.05). However, AC II activity was abolished when combined with naloxone. In wire hang test demonstrated no difference between falling score (p: 0.053) and reaching score (0.903), that showed AC-II wasn’t impaired the motoric system. No death or toxicity symptoms were observed for 14 days in the acute toxicity study. A. conyzoides aqueous extract is potential for anti-neuropathic pain..Nyeri neuropatik merupakan kondisi yang sulit untuk ditangani dan mengakibatkan penurunan kualitas hidup. Penelitian bertujuan untuk mengetahui efek rebusan daun Ageratum conyzoides L (AC) sebagai anti-nyeri neuropatik disertai profil keamanannya. Hewan percobaan (mencit) dikelompokkan menjadi tujuh kelompok dengan masing-masing terdiri dari 5 hewan. Pada semua kelompok diberikan induksi piridoksin (400 mg/Kb BB) selama 14 hari kecuali kelompok normal, yang diikuti pemberian sediaan sesuai dengan kelompoknya serta dilanjutkan dengan pengujian thermal allodynia, hiperlagesia, dan wire hang. Pengujian toksisitas akut menggunakan tikus dengan menggunakan dosis 2000 mg/kg BB. Hasil thermal allodynia dan hiperalgesia menunjukkan AC-II memberikan efek peningkatan threshold pain lebih baik dibandingkan AC-I. Pada kombinasi AC-II dan pregabalin memberikan efek paling baik dibandingkan dengan kelompok lainnya dan menunjukkan efek sinergistik. Akan tetapi, efek AC-II mengalami penurunan (reversal) bila dikombinasikan dengan naloxone, hal ini menunjukkan adanya keterlibatan pada reseptor opioid (µ, κ and σ). Pengujian wire hang menunjukkan bahwa tidak terdapat perbedaan dalam falling score (p;0.053) dan reaching score (p: 0.903), hal ini menunjukkan bahwa AC-II tidak mengakibatkan gangguan terhadap fungsi motorik. Pada pengujian toksisitas akut, tidak ditemukan gejala toksisitas ataupun kematian terhadap hewan percobaan. Berdasarkan hal tersebut maka dapat disimpulkan bahwa rebusan daun babadotan berpotensi sebagai anti-nyeri neuropatik

HAEMOSTATIC EFFECT OF ETHANOLIC EXTRACT OF Ageratum conyzoides L TO STRAINS OF MICE MALE SWISS WEBSTER INDUCED WITH COMBINATION OF ASPIRIN, CLOPIDOGREL AND ENOXAPARIN

Objective: Bleeding complications are a common concern with the use of combination of antiagregations and anticoagulant agents especially on acute coronary syndrome treatment. In selected situations such as life-threatening, reversing may be desired, but no specific antidotum for the newer agents such as enoxaparin, fondaparinux, levirudin, bivalirudin, and argatroban. Ageratum conyzoides L is a medicinal plants that have a hemostatic effect. The objective of the study is to know the hemostatic effect of A. conyzoides L to induction of acetosal, clopidogrel, and enoxaparin.Methods: A total of 20 mice are divided into five groups that are normal, negative, positive, Test I and II. There were no treatment for normal, induction of drugs combination for negative, induction of drugs combination and tranexamic acid for positive, induction of drugs combination and ethanolic extract of A. conyzoides L (100 mg/Kg BW) for Test I, induction of drugs combination and ethanolic extract of A. conyzoides L (250 mg/Kg BW) for Test II.Results: The Test Groups I and II showed reversing of clotting time to normal baseline and shown a significant difference (p<0.05) compared with the negative group. In addition, the Test Groups I and II showed significant difference (p<0.05) of bleeding time compared with the negative group and the Test II (250 mg/Kg BW) shown reversing of bleeding time to normal baseline.Conclusion: The hemostatic effect showed by ethanolic extract of A. conyzoides L to induction of combination acetosal, clopidogrel and enoxaparin are very valuable for reversing agent novel

Phytochemical compounds and pharmacological activities of Ipomoea batatas L.: An updated review

This article provides an overview of Ipomoea batatas L., the Ipomoea genus. The review covers traditional uses, nutritional value, phytochemical compounds, pharmacological activities, and toxicity studies. Data were collected from scientific databases and search engines. Sweet potatoes are used in various countries for traditional uses such as dietary fiber sources, treating allergies, and providing energy in diabetes mellitus treatment. The primary phytochemical compounds in Ipomoea batatas are phenolic compounds, flavonoids, anthocyanins, and carotenoids. Sweet potato contains several nutritional constituents: vitamin C, protein, fiber, carbohydrates, β-carotene, and minerals. Sweet potato exhibits various pharmacological activities, such as antioxidant, aphrodisiac, anticancer, and anti-inflammatory. The types of phytochemical compounds in each part of the plant are different. Each pharmacological activity and mechanism of action depends on the phytochemical compounds, part and variety of the plant, and extraction solvent. However, further study is required to investigate the chronic toxicity of Ipomoea batatas