Band termination in the N=Z Odd-Odd Nucleus 46V

High spin states in the odd-odd N=Z nucleus 46V have been identified. At low spin, the T=1 isobaric analogue states of 46Ti are established up to I = 6+. Other high spin states, including the band terminating state, are tentatively assigned to the same T=1 band. The T=0 band built on the low-lying 3+ isomer is observed up to the 1f7/2-shell termination at I=15. Both signatures of a negative parity T=0 band are observed up to the terminating states at I = 16- and I = 17-, respectively. The structure of this band is interpreted as a particle-hole excitation from the 1d3/2 shell. Spherical shell model calculations are found to be in excellent agreement with the experimental results.Comment: 5 pages, 4 figure

Natural deep eutectic solvent (NADES): A strategy to improve the bioavailability of blueberry phenolic compounds in a ready-to-use extract.

This study investigated whether a ready-to-use extract obtained using a natural deep eutectic solvent (NADES) affects the pharmacokinetic profile of blueberry phenolic compounds compared to organic solvent (SORG)-extracted compounds. SORG extract was administered as an aqueous solution after solvent removal. Wistar rats received a single dose of crude extract of blueberry obtained using NADES (CE-NADES) or SORG (CE-SORG), followed by LC-DAD-MS/MS analysis of blood and cecal feces. Non-compartmental pharmacokinetic analysis revealed that CE-NADES increased the bioavailability of anthocyanins by 140% compared to CE-SORG. CE-NADES increased the stability of phenolic compounds during in vitro digestion by delaying gastric chyme neutralization. These results suggest that besides being an eco-friendly solvent for the extraction of phytochemicals, choline chloride:glycerol:citric acid-based NADES can be used as a ready-to-use vehicle for increasing oral absorption of bioactive compounds such as anthocyanins

Natural deep eutectic solvent (NADES)-based blueberry extracts protect against ethanol-induced gastric ulcer in rats.

Blueberry is a polyphenol-rich fruit bearing great bioactive potential. Natural deep eutectic solvents (NADES) emerged as putatively biocompatible solvents that could substitute for toxic organic solvents in the extraction of fruit phenolic compounds for developing nutraceuticals or functional foods. Therefore, the aim of this study was to investigate the gastroprotective effects and the biocompatibility of a blueberry crude extract (CE) obtained using NADES and of the extract fractions (anthocyanin-rich fraction ? ARF; non-anthocyanin phenolic fraction - NAPF) in a model of ethanol-induced gastric ulcer in rats. CE was the NADES-containing, ready-to-use extract that was obtained using choline chloride:glycerol:citric acid NADES (0.5:2:0.5 M ratio). ARF and NAPF were the NADES-free fractions obtained by solid phase purification of CE and were investigated to identify the bioactive fraction responsible for the effects of CE. Animals were treated for 14 days with water, NADES vehicle, CE, ARF, NAPF or lansoprazole (intragastric) and then received ethanol to induce gastric ulcer. CE decreased ulcer index and preserved the integrity of gastric mucosa. The pretreatment with CE or ARF reduced glutathione depletion and the inflammatory response. All treatments, including NADES vehicle reduced protein oxidation and nitric oxide overproduction in ethanol-treated rats. Additionally, ARF increased short-chain fatty acids in feces. These findings suggest that NADES can be used to obtain biocompatible extracts of blueberry that exhibit gastroprotective effects with no need of solvent removal. The gastroprotective effects were mainly associated to ARF but NAPF and even NADES vehicle also contributed to some protective effects

A utiliza??o do cimento de fosfato de c?lcio como arcabou?o para engenharia de tecido ?sseo : estudo in vitro

Made available in DSpace on 2015-04-14T13:29:26Z (GMT). No. of bitstreams: 1 413419.pdf: 7548238 bytes, checksum: fb7df37e9d8df24acf3390709418323f (MD5) Previous issue date: 2009-03-31A engenharia tecidual tem se mostrado um campo promissor para a reabilita??o de pacientes com traumas ou deformidades faciais que necessitem de ?reas extensas de enxerto ?sseo. Seu princ?pio b?sico envolve a associa??o de um tipo apropriado de c?lula e de um arcabou?o biocompat?vel e bioabsorv?vel que mimetize funcional e estruturalmente determinado tipo de tecido. O Cimento de Fosfato de C?lcio (CFC) aparece como uma alternativa vi?vel de biomaterial a ser utilizado com esta finalidade em virtude de suas caracter?sticas como biocompatibilidade, bioatividade e osteocondutividade. Assim, o objetivo da presente pesquisa foi avaliar a capacidade de suportes tridimensionais macroporosos de CFC, confeccionados com mat?rias-primas brasileiras, de permitir a ades?o, prolifera??o e diferencia??o de c?lulas-tronco mesenquimais derivadas da medula ?ssea humana. Para isso, c?lulas obtidas da crista il?aca de um doador humano adulto foram rotineiramente processadas at? as condi??es experimentais e, a seguir, cultivadas sobre suportes de CFC, macroporosos, que tiveram como corpo gerador de poros, microesferas de parafina, com tamanho entre 100 e 250μm. Para indu??o das c?lulas em linhagem osteog?nica foi acrescentada Prote?na Morfog?nica ?ssea (BMP4) ao meio de cultura. Os per?odos de cultura estabelecidos foram de cinco, dez e 15 dias. Ap?s estes per?odos, o comportamento e a morfologia das c?lulas junto ao biomaterial foram avaliados por meio de Microscopia Eletr?nica de Varredura. Os n?veis de express?o dos genes BGLA e SSP1, que codificam as prote?nas osteocalcina (OC) e osteopontina (OP) respectivamente, bem como a atividade da Fosfatase Alcalina (ALP), outro marcador osteobl?stico importante, foram quantificados pela t?cnica de PCR em Tempo Real (QT-PCR) utilizando o fluor?foro SYBR Green?. O QT-PCR detectou a express?o dos genes BGLA e SSP1 e a atividade da fosfatase alcalina nos per?odos de 10 e 15 dias de cultura, o que nos permitiu constatar que houve prolifera??o celular e diferencia??o destas em c?lulas osteog?nicas. No per?odo de cinco dias, n?o foi observada a express?o de nenhum dos genes investigados. A partir destes resultados conclu?mos que o CFC, macroporoso, com tamanho de poros entre 100 e 250μm, criados por meio da utiliza??o de microesferas de parafina, permite a ades?o, prolifera??o e diferencia??o de c?lulas-tronco mesenquimais em c?lulas osteog?nicas, podendo ser utilizado como arcabou?o para engenharia de tecido ?sseo

The hepatoprotective effect of jaboticaba peel powder in a rat model of type 2 diabetes mellitus involves the modulation of thiol/disulfide redox state through the upregulation of glutathione synthesis

Jaboticaba peel powder (JPP) is rich in bioactive compounds, mainly soluble and insoluble polyphenols with great antioxidant properties. The aim of this study is to evaluate the effects of JPP supplementation on the oxidative stress and hepatic damage in a rat model of type 2 diabetes mellitus (T2DM). Diabetic rats received vehicle or JPP at 2.7 (JPP-I), 5.4 (JPP-II), or 10.8 (JPP-III) g/L in drinking water during 8 weeks. JPP-III attenuated hyperglycaemia and dyslipidemia increased by 86% the liver content of nonprotein thiol groups and by 90% the GSH/GSSG ratio by activating glutathione synthesis. Accordingly, JPP supplementation prevented the loss of activity of the sulfhydryl-dependent enzyme δ-aminolaevulinic acid dehydratase and attenuated hepatic injury assessed by the reduction of serum aspartate aminotransferase activity and liver hypertrophy. Our results support that JPP supplementation to T2DM rats decreases hepatic damage most likely by increasing glutathione synthesis and modulating the thiol/disulfide redox balance.2018CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES309227/2013-5 ; 458664/2014-6 ; 301108/2016-1 ; 552440/2011-6Sem informaçã

Alternating parity bands in 218 87 Fr

States in doubly odd ${}^{218}\mathrm{Fr}$ have been studied using in-beam spectroscopy \ensuremath{\alpha}\ensuremath{-}\ensuremath{\gamma}\ensuremath{-}\ensuremath{\gamma} coincidence techniques mainly through the ${}^{209}\mathrm{Bi}{(}^{18}\mathrm{O},$ 2\ensuremath{\alpha}n) reaction at 94 MeV bombarding energy, using the 8\ensuremath{\pi} GASP-ISIS spectrometer at Legnaro. ${}^{218}\mathrm{Fr}$ shows a band structure, with interleaved states of alternating parities connected by enhanced $E1$ transitions. Tentative spin assignment and the relation between the structure of ${}^{218}\mathrm{Fr}$ and its isotone ${}^{220}\mathrm{Ac}$ is discussed