138 research outputs found
Focusing and Compression of Ultrashort Pulses through Scattering Media
Light scattering in inhomogeneous media induces wavefront distortions which
pose an inherent limitation in many optical applications. Examples range from
microscopy and nanosurgery to astronomy. In recent years, ongoing efforts have
made the correction of spatial distortions possible by wavefront shaping
techniques. However, when ultrashort pulses are employed scattering induces
temporal distortions which hinder their use in nonlinear processes such as in
multiphoton microscopy and quantum control experiments. Here we show that
correction of both spatial and temporal distortions can be attained by
manipulating only the spatial degrees of freedom of the incident wavefront.
Moreover, by optimizing a nonlinear signal the refocused pulse can be shorter
than the input pulse. We demonstrate focusing of 100fs pulses through a 1mm
thick brain tissue, and 1000-fold enhancement of a localized two-photon
fluorescence signal. Our results open up new possibilities for optical
manipulation and nonlinear imaging in scattering media
Recovering three-dimensional shape around a corner using ultrafast time-of-flight imaging
The recovery of objects obscured by scattering is an important goal in imaging and has been approached by exploiting, for example, coherence properties, ballistic photons or penetrating wavelengths. Common methods use scattered light transmitted through an occluding material, although these fail if the occluder is opaque. Light is scattered not only by transmission through objects, but also by multiple reflection from diffuse surfaces in a scene. This reflected light contains information about the scene that becomes mixed by the diffuse reflections before reaching the image sensor. This mixing is difficult to decode using traditional cameras. Here we report the combination of a time-of-flight technique and computational reconstruction algorithms to untangle image information mixed by diffuse reflection. We demonstrate a three-dimensional range camera able to look around a corner using diffusely reflected light that achieves sub-millimetre depth precision and centimetre lateral precision over 40 cm×40 cm×40 cm of hidden space.MIT Media Lab ConsortiumUnited States. Defense Advanced Research Projects Agency. Young Faculty AwardMassachusetts Institute of Technology. Institute for Soldier Nanotechnologies (Contract W911NF-07-D-0004
High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging
Progress in neuroscience constantly relies on the development of new
techniques to investigate the complex dynamics of neuronal networks. An ongoing
challenge is to achieve minimally-invasive and high-resolution observations of
neuronal activity in vivo inside deep brain areas. A perspective strategy is to
utilise holographic control of light propagation in complex media, which allows
converting a hair-thin multimode optical fibre into an ultra-narrow imaging
tool. Compared to current endoscopes based on GRIN lenses or fibre bundles,
this concept offers a footprint reduction exceeding an order of magnitude,
together with a significant enhancement in resolution. We designed a compact
and high-speed system for fluorescent imaging at the tip of a fibre, achieving
micron-scale resolution across a 50 um field of view, and yielding 7-kilopixel
images at a rate of 3.5 frames/s. Furthermore, we demonstrate in vivo
observations of cell bodies and processes of inhibitory neurons within deep
layers of the visual cortex and hippocampus of anesthetised mice. This study
forms the basis for several perspective techniques of modern microscopy to be
delivered deep inside the tissue of living animal models while causing minimal
impact on its structural and functional properties.Comment: 10 pages, 2 figures, Supplementary movie:
https://drive.google.com/file/d/1Fm0G3TAIC49LVX6FaEiAtlefkWx1T2a5/vie
Speckle-scale focusing in the diffusive regime with time reversal of variance-encoded light (TROVE)
Focusing of light in the diffusive regime inside scattering media has long been considered impossible. Recently, this limitation has been overcome with time reversal of ultrasound-encoded light (TRUE), but the resolution of this approach is fundamentally limited by the large number of optical modes within the ultrasound focus. Here, we introduce a new approach, time reversal of variance-encoded light (TROVE), which demixes these spatial modes by variance encoding to break the resolution barrier imposed by the ultrasound. By encoding individual spatial modes inside the scattering sample with unique variances, we effectively uncouple the system resolution from the size of the ultrasound focus. This enables us to demonstrate optical focusing and imaging with diffuse light at an unprecedented, speckle-scale lateral resolution of ~5 µm
Modal beam splitter:Determination of the transversal components of an electromagnetic light field
The transversal profile of beams can always be defined as a superposition of orthogonal fields, such as optical eigenmodes. Here, we describe a generic method to separate the individual components in a laser beam and map each mode onto its designated detector with low crosstalk. We demonstrate this with the decomposition into Laguerre-Gaussian beams and introduce a distribution over the integer numbers corresponding to the discrete orbital and radial momentum components of the light field. The method is based on determining an eigenmask filter transforming the incident optical eigenmodes to position eigenmodes enabling the detection of the state of the light field using single detectors while minimizing cross talk with respect to the set of filter masks considered.UK Engineering and Physical Sciences Research Council [EP/J01771X/1]This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
Wavefront shaping with disorder-engineered metasurfaces
Recently, wavefront shaping with disordered media has demonstrated optical manipulation capabilities beyond those of conventional optics, including extended volume, aberration-free focusing and subwavelength focusing. However, translating these capabilities to useful applications has remained challenging as the input–output characteristics of the disordered media (P variables) need to be exhaustively determined via O(P) measurements. Here, we propose a paradigm shift where the disorder is specifically designed so its exact input–output characteristics are known a priori and can be used with only a few alignment steps. We implement this concept with a disorder-engineered metasurface, which exhibits additional unique features for wavefront shaping such as a large optical memory effect range in combination with a wide angular scattering range, excellent stability, and a tailorable angular scattering profile. Using this designed metasurface with wavefront shaping, we demonstrate high numerical aperture (NA > 0.5) focusing and fluorescence imaging with an estimated ~2.2 × 10^8 addressable points in an ~8 mm field of view
Scattering invariant modes of light in complex media
Random scattering of light in disordered media is an intriguing phenomenon of
fundamental relevance to various applications. While techniques such as
wavefront shaping and transmission matrix measurements have enabled remarkable
progress for advanced imaging concepts, the most successful strategy to obtain
clear images through a disordered medium remains the filtering of ballistic
light. Ballistic photons with a scattering-free propagation are, however,
exponentially rare and no method so far can increase their proportion. To
address these limitations, we introduce and experimentally implement here a new
set of optical states that we term Scattering Invariant Modes (SIMs), whose
transmitted field pattern is the same, irrespective of whether they scatter
through a disordered sample or propagate ballistically through a homogeneous
medium. We observe SIMs that are only weakly attenuated in dense scattering
media, and show in simulations that their correlations with the ballistic light
can be used to improve imaging inside scattering materials
Three-dimensional holographic optical manipulation through a high-numerical-aperture soft-glass multimode fibre
Holographic optical tweezers (HOT) hold great promise for many applications in biophotonics, allowing the creation and measurement of minuscule forces on biomolecules, molecular motors and cells. Geometries used in HOT currently rely on bulk optics, and their exploitation in vivo is compromised by the optically turbid nature of tissues. We present an alternative HOT approach in which multiple three-dimensional (3D) traps are introduced through a high-numerical-aperture multimode optical fibre, thus enabling an equally versatile means of manipulation through channels having cross-section comparable to the size of a single cell. Our work demonstrates real-time manipulation of 3D arrangements of micro-objects, as well as manipulation inside otherwise inaccessible cavities. We show that the traps can be formed over fibre lengths exceeding 100 mm and positioned with nanometric resolution. The results provide the basis for holographic manipulation and other high-numerical-aperture techniques, including advanced microscopy, through single-core-fibre endoscopes deep inside living tissues and other complex environments
Shaping speckles: spatio-temporal focussing of an ultrafast pulse through a multiply scattering medium
The multiple scattering of coherent light is a problem of both fundamental
and applied importance. In optics, phase conjugation allows spatial focussing
and imaging through a multiply scattering medium; however, temporal control is
nonetheless elusive, and multiple scattering remains a challenge for
femtosecond science. Here, we report on the spatially and temporally resolved
measurement of a speckle field produced by the propagation of an ultrafast
optical pulse through a thick strongly scattering medium. Using spectral pulse
shaping, we demonstrate the spatially localized temporal recompression of the
output speckle to the Fourier-limit duration, offering an optical analogue to
time-reversal experiments in the acoustic regime. This approach shows that a
multiply scattering medium can be put to profit for light manipulation at the
femtosecond scale, and has a diverse range of potential applications that
includes quantum control, biological imaging and photonics.Comment: 7 pages, 3 figures, published in Nature Communication
A polycystic variant of a primary intracranial leptomeningeal astrocytoma: case report and literature review
<p>Abstract</p> <p>Background</p> <p>Primary leptomeningeal astrocytomas are rare intracranial tumors. These tumors are believed to originate from cellular nests which migrate by means of aberration, ultimately settling in the leptomeningeal structure. They may occur in both solitary and diffuse forms. The literature reports only fifteen cases of solitary primary intracranial leptomeningeal astrocytomas.</p> <p>Case presentation</p> <p>The authors report the case of a seventy-eight year-old woman with a polycystic variant of a solitary primary intracranial leptomeningeal astrocytoma. The first neurological signs were seizures and aphasia. CT and MRI scans demonstrated a fronto-parietal polycystic tumor adherent to the sub arachnoid space. A left fronto-temporo-parietal craniotomy revealed a tight coalescence between the tumor and the arachnoid layer which appeared to wrap the mass entirely. Removal of the deeper solid part of the tumor resulted difficult due to the presence of both a high vascularity and a tight adherence between the tumor and the ventricular wall.</p> <p>Conclusion</p> <p>A new case of a solitary primitive intracranial leptomeningeal astrocytoma of a rare polycystic variant is reported. Clinical, surgical, pathologic and therapeutic aspects of this tumor are discussed.</p
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