Adventitious shoot regeneration from leaf explants of the valuable medicinal herb Plectranthus barbatus Andrews

The objective of this study was to develop an efficient protocol for adventitious shoot regeneration for Plectranthus barbatus Andrews using leaf explants. The explants were cultured on MS (Murashige and Skoog, 1962) medium containing various concentration of kinetin (KN), 6-benzylaminopurine (BAP) and thidiazuron (TDZ) (0.5 to 3.0 mg/l) or in combination with á-naphthalene acetic acid (NAA) for shoot development. The highest adventitious shoot regeneration percentage (80%) and average maximum number of 19.7 ± 2.08 shoots per explants was observed on MS medium supplemented with 1.5 mg/l KN followed by 2.0 mg/l BAP with 1.0 mg/l NAA (15.0 ± 2.20 shoots per explants). The shoots were excised and the residual explants were transferred to fresh medium where again they developed shoots. The shoots were elongated when they were cultured on half strength MS medium supplemented with 0.6 mg/l gibberellic acid (GA3) and 0.5 mg/l KN. The in vitro developed shoots produced roots when transferred to half strength MS medium augmented with 1.5 mg/l indole-3-butyric acid (IBA). The in vitro raised plantlets were successfully transferred to mixture of river sand, garden soil and saw dust (1:1:1) and was hardened in controlled environment. Hardened plantlets were transferred to soil in greenhouse.Key words: Plectranthus barbatus, Lamiaceae, medicinal plant, leaf explants, direct organogenesis, in vitro, plant growth regulators

On-Shell Recursion Relations for Generic Theories

We show that on-shell recursion relations hold for tree amplitudes in generic two derivative theories of multiple particle species and diverse spins. For example, in a gauge theory coupled to scalars and fermions, any amplitude with at least one gluon obeys a recursion relation. In (super)gravity coupled to scalars and fermions, the same holds for any amplitude with at least one graviton. This result pertains to a broad class of theories, including QCD, N=4 SYM, and N=8 supergravity.Comment: 19 pages, 3 figure

Boundary Contributions Using Fermion Pair Deformation

Continuing the study of boundary BCFW recursion relation of tree level amplitudes initiated in \cite{Feng:2009ei}, we consider boundary contributions coming from fermion pair deformation. We present the general strategy for these boundary contributions and demonstrate calculations using two examples, i.e, the standard QCD and deformed QCD with anomalous magnetic momentum coupling. As a by-product, we have extended BCFW recursion relation to off-shell gluon current, where because off-shell gluon current is not gauge invariant, a new feature must be cooperated.Comment: 26 pages, 4 figure

Dual conformal constraints and infrared equations from global residue theorems in N=4 SYM theory

Infrared equations and dual conformal constraints arise as consistency conditions on loop amplitudes in N=4 super Yang-Mills theory. These conditions are linear relations between leading singularities, which can be computed in the Grassmannian formulation of N=4 super Yang-Mills theory proposed recently. Examples for infrared equations have been shown to be implied by global residue theorems in the Grassmannian picture. Both dual conformal constraints and infrared equations are mapped explicitly to global residue theorems for one-loop next-to-maximally-helicity-violating amplitudes. In addition, the identity relating the BCFW and its parity-conjugated form of tree-level amplitudes, is shown to emerge from a particular combination of global residue theorems.Comment: 21 page

Complete Equivalence Between Gluon Tree Amplitudes in Twistor String Theory and in Gauge Theory

The gluon tree amplitudes of open twistor string theory, defined as contour integrals over the ACCK link variables, are shown to satisfy the BCFW relations, thus confirming that they coincide with the corresponding amplitudes in gauge field theory. In this approach, the integration contours are specified as encircling the zeros of certain constraint functions that force the appropriate relation between the link variables and the twistor string world-sheet variables. To do this, methods for calculating the tree amplitudes using link variables are developed further including diagrammatic methods for organizing and performing the calculations.Comment: 38 page

General Split Helicity Gluon Tree Amplitudes in Open Twistor String Theory

We evaluate all split helicity gluon tree amplitudes in open twistor string theory. We show that these amplitudes satisfy the BCFW recurrence relations restricted to the split helicity case and, hence, that these amplitudes agree with those of gauge theory. To do this we make a particular choice of the sextic constraints in the link variables that determine the poles contributing to the contour integral expression for the amplitudes. Using the residue theorem to re-express this integral in terms of contributions from poles at rational values of the link variables, which we determine, we evaluate the amplitudes explicitly, regaining the gauge theory results of Britto et al.Comment: 30 pages, minor misprints correcte

Generating MHV super-vertices in light-cone gauge

We constructe the $\mathcal{N}=1$ SYM lagrangian in light-cone gauge using chiral superfields instead of the standard vector superfield approach and derive the MHV lagrangian. The canonical transformations of the gauge field and gaugino fields are summarised by the transformation condition of chiral superfields. We show that $\mathcal{N}=1$ MHV super-vertices can be described by a formula similar to that of the $\mathcal{N}=4$ MHV super-amplitude. In the discussions we briefly remark on how to derive Nair's formula for $\mathcal{N}=4$ SYM theory directly from light-cone lagrangian.Comment: 25 pages, 7 figures, JHEP3 style; v2: references added, some typos corrected; Clarification on the condition used to remove one Grassmann variabl

Manifest SO(N) invariance and S-matrices of three-dimensional N=2,4,8 SYM

An on-shell formalism for the computation of S-matrices of SYM theories in three spacetime dimensions is presented. The framework is a generalization of the spinor-helicity formalism in four dimensions. The formalism is applied to establish the manifest SO(N) covariance of the on-shell superalgebra relevant to N =2,4 and 8 SYM theories in d=3. The results are then used to argue for the SO(N) invariance of the S-matrices of these theories: a claim which is proved explicitly for the four-particle scattering amplitudes. Recursion relations relating tree amplitudes of three-dimensional SYM theories are shown to follow from their four-dimensional counterparts. The results for the four-particle amplitudes are verified by tree-level perturbative computations and a unitarity based construction of the integrand corresponding to the leading perturbative correction is also presented for the N=8 theory. For N=8 SYM, the manifest SO(8) symmetry is used to develop a map between the color-ordered amplitudes of the SYM and superconformal Chern-Simons theories, providing a direct connection between on-shell observables of D2 and M2-brane theories.Comment: 28 page

Spinor Helicity and Dual Conformal Symmetry in Ten Dimensions

The spinor helicity formalism in four dimensions has become a very useful tool both for understanding the structure of amplitudes and also for practical numerical computation of amplitudes. Recently, there has been some discussion of an extension of this formalism to higher dimensions. We describe a particular implementation of the spinor-helicity method in ten dimensions. Using this tool, we study the tree-level S-matrix of ten dimensional super Yang-Mills theory, and prove that the theory enjoys a dual conformal symmetry. Implications for four-dimensional computations are discussed.Comment: 24 pages, 1 figure

Note on New KLT relations

In this short note, we present two results about KLT relations discussed in recent several papers. Our first result is the re-derivation of Mason-Skinner MHV amplitude by applying the S_{n-3} permutation symmetric KLT relations directly to MHV amplitude. Our second result is the equivalence proof of the newly discovered S_{n-2} permutation symmetric KLT relations and the well-known S_{n-3} permutation symmetric KLT relations. Although both formulas have been shown to be correct by BCFW recursion relations, our result is the first direct check using the regularized definition of the new formula.Comment: 15 Pages; v2: minor correction