PREFERÊNCIA DE EQÜINOS EM PASTEJO: EFEITO DA ALTURA DE DOSSÉIS DE GRAMÍNEAS DO GÊNERO CYNODON

The experiment was conducted at the experimental farm of the Federal University of Paraná, in February, 1998, and investigated the effect of the different Cynodon grasses structure at the horse grazing. The Cynodon spp. cv Tifton 85, Cynodon nlemfuensis cv Tifton 68, Cynodon spp. cv Tifton 44, Cynodon nlemfuensis (¨Porto Rico¨) and Cynodon nlemfuensis (¨Estrela Roxa¨) grasses were distributed in parcels with 12 m2 in a completely randomized split-plot design with four replications. The parcels were divided in two kinds of patches (tall and short) demonstrated by plants height. Five grazing tests were done with mares, evaluating the number of bites allocated in each treatment. The preference was evaluated by bite number and tiller grazed on each treatment. The bite depth was measured using 20 marked tillers in each experimental unit. The animals showed preference for Tifton 85, followed by Coastcross 1, Tifton 68 and Tifton 44. The star grasses ¨Estrela Roxa¨ and ¨Porto Rico¨ were not preferred. Tifton 85 and Coastcross 1 with more leaves and less stems mass (PO experimento foi conduzido no Centro de Estações Experimentais da Universidade Federal do Paraná, em fevereiro de 1998, quando as gramíneas Cynodon spp. cv Tifton 85, Cynodon nlemfuensis cv Tifton 68, Cynodon spp. cv Tifton 44, Cynodon nlemfuensis (Porto Rico) e Cynodon nlemfuensis (Estrela Roxa) foram estabelecidas em parcelas em um delineamento inteiramente casualizado com parcelas subdivididas e quatro repetições. As parcelas foram subdivididas em duas alturas do dossel (alto e baixo) caracterizadas pela diferença de altura das plantas. Os tratamentos foram ofertados a três éguas por duas horas em cinco dias consecutivos. A preferência entre os tratamentos foi identificada pela avaliação do número de bocados e pela desfolha nas unidades vegetativas marcadas nas parcelas e alturas. A profundidade do bocado foi quantificada por meio da identificação da altura do perfilho estendido e da menor altura pastejada nos perfilhos marcados das diferentes gramíneas. A Tifton 85 foi preferida, seguida pela Coastcross 1, Tifton 68 e Tifton 44. A Estrela Roxa e a Porto Rico foram preteridas. As gramíneas de maior preferência apresentavam maior massa de folhas verdes em relação à massa de colmos (

Expanded Bed Adsorption Of Bromelain (e.c. 3.4.22.33) From Ananas Comosus Crude Extract

This work focuses on the adsorption of Bromelain in expanded bed conditions, such as the adsorption kinetics parameters. The adsorption kinetics parameters showed that after 40 minutes equilibrium was achieved and maximum adsorption capacity was 6.11 U per resin mL. However, the maximum adsorption capacity was only determined by measuring the adsorption isotherm. Only by the Langmuir model the maximum adsorption capacity, Qm, and dissociation constant, kd, values could be estimated as 9.18 U/mL and 0.591, respectively, at 25°C and 0.1 mol/L phosphate buffer pH 7.5. A column made of glass with an inner diameter of 1 cm was used for the expanded bed adsorption (EBA). The residence time was reduced 10 fold by increasing the expansion degree 2.5 times; nonetheless, the plate number (N) value was reduced only 2 fold. After adsorption, the bromelain was eluted in packed bed mode, with a downward flow. The purification factor was about 13 fold and the total protein was reduced 4 fold. 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Gota úrica visceral em coruja suindara (Tyto alba) de vida livre

A gota úrica é uma doença metabólica caracterizada pela deposição de cristais de urato e ácido úrico em tecidos do corpo, que pode acometer répteis, mamíferos e aves. Neste trabalho, relata-se um caso de achados post mortem compatíveis com gota úrica visceral em um exemplar de coruja suindara (Tyto alba) de vida livre. Macroscopicamente, o pericárdio apresentava-se completamente esbranquiçado, espesso e aderido ao miocárdio e o fígado e ambos os rins apresentavam áreas esbranquiçados sugerindo acúmulo de urato. A microscopia do tecido cardíaco e hepático revelou pericardite fibrinosa e perihepatite necrosante respectivamente. Congestão pulmonar e infiltrado inflamatório heterofílico multifocal no parênquima renal também foram visualizados. As alterações encontradas são compatíveis com as descritas na literatura para gota úrica visceral em aves, podendo até serem consideradas como lesões patognomônicas. Este é o primeiro relato de gota úrica visceral em um rapinante de vida livre no Brasil