78 research outputs found
Vegetative development and content of calcium, potassium, and sodium in watermelon under salinity stress on organic substrates
Isolamento de isobruceina B e neosergeolida de Picrolemma sprucei Hook. f. em escala-grama
Quassinoids neosergeolide and isobrucein B, obtained from Picrolemma sprucei, have proven in vitro antitumor, antimalarial, anthelminthic, cytotoxic, insecticide and leishmanicidal activities. There is interest in the in vivo pharmacological study of these natural compounds and their semi-synthetic derivatives, however, the quantities obtained in previous extraction processes have been shown to be a limiting factor for continuation of these studies. Herein, we describe a method for obtaining grams of these quassinoids whose purification relies only on recrystallization.Os quassinĂłides neosergeolida e isobruceĂna B, obtidos de Picrolemma sprucei, possuem atividades antitumoral, antimalĂĄrica, anti-helmĂntica, citotĂłxica, inseticida e anti-leishmania comprovadas em estudos in vitro. HĂĄ interesse no estudo farmacolĂłgico in vivo dessas substĂąncias naturais e de seus derivados semi-sintĂ©ticos, porĂ©m a quantidade obtida nos processos de extração tem se mostrado um fator limitante Ă continuação desses estudos. No presente trabalho, descrevemos um mĂ©todo para obtenção de gramas desses quassinĂłides cuja purificação depende apenas de cristalização fracionada
Highlights of the SĂŁo Paulo ISEV workshop on extracellular vesicles in cross-kingdom communication
In the past years, extracellular vesicles (EVs) have become an important field of research since EVs have been found to play a central role in biological processes. In pathogens, EVs are involved in several events during the hostâpathogen interaction, including invasion, immunomodulation, and pathology as well as parasiteâparasite communication. In this report, we summarised the role of EVs in infections caused by viruses, bacteria, fungi, protozoa, and helminths based on the talks and discussions carried out during the International Society for Extracellular Vesicles (ISEV) workshop held in SĂŁo Paulo (November, 2016), Brazil, entitled Cross-organism Communication by Extracellular Vesicles: Hosts, Microbes and Parasites. © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.11Ysciescopu
Tratamento térmico de frutos da cajazeira utilizando vapor d'ågua, visando ao controle de Ceratitis capitata e à qualidade do fruto
Seleção de cultivares de laranja doce de maturação precoce por Ăndices de desempenho
Muscularity and adiposity of carcass of Santa InĂȘs lambs: effects of different levels of replacement of ground corn by forage cactus meal in finishing ration
Hypotensive effect and endothelium-dependent vascular action of leaves of Alpinia purpurata (Vieill) K. Schum
The aims of this study were to evaluate the chemical profile, vascular reactivity, and acute hypotensive effect (AHE) of the ethanolic extract of leaves of Alpinia purpurata (Vieill) K. Schum (EEAP). Its chemical profile was evaluated using HPLC-UV, ICP-OES, and colorimetric quantification of total flavonoids and polyphenols. The vascular reactivity of the extract was determined using the mesenteric bed isolated from WKY. AHE dose-response curves were obtained for both EEAP and inorganic material isolated from AP (IAP) in WKY and SHR animals. Cytotoxic and mutagenic safety levels were determined by the micronucleus test. Rutin-like flavonoids were quantified in the EEAP (1.8 ± 0.03%), and the total flavonoid and polyphenol ratios were 4.1 ± 1.8% and 5.1 ± 0.3%, respectively. We observed that the vasodilation action of EEAP was partially mediated by nitric oxide (·NO). The IAP showed the presence of calcium (137.76 ± 4.08 Όg mg-1). The EEAP and IAP showed an AHE in WKY and SHR animals. EEAP did not have cytotoxic effects or cause chromosomic alterations. The AHE shown by EEAP could result from its endothelium-dependent vascular action. Rutin-like flavonoids, among other polyphenols, could contribute to these biological activities, and the calcium present in EEAP could act in a synergistic way
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