Micromechanical modeling of tension stiffening in FRP-strengthened concrete elements

This article presents a micromodeling computational framework for simulating the tensile response and tension-stiffening behavior of fiber reinforced polymer–strengthened reinforced concrete elements. The total response of strengthened elements is computed based on the local stress transfer mechanisms at the crack plane including concrete bridging stress, reinforcing bars stress, FRP stress, and the bond stresses at the bars-to-concrete and fiber reinforced polymer-to-concrete interfaces. The developed model provides the possibility of calculating the average response of fiber reinforced polymer, reinforcing bars, and concrete as well as the crack spacing and crack widths. The model, after validation with experimental results, is used for a systematic parameter study and development of micromechanics-based relations for calculating the crack spacing, fiber reinforced polymer critical ratio, debonding strength, and effective bond length. Constitutive models are also proposed for concrete tension stiffening and average response of steel reinforcing bars in fiber reinforced polymer–strengthened members as the main inputs of smeared crack modeling approaches

Preparation and Characterization of Cationic PLA-PEG Nanoparticles for Delivery of Plasmid DNA

The purpose of the present work was to formulate and evaluate cationic poly(lactic acid)-poly(ethylene glycol) (PLA-PEG) nanoparticles as novel non-viral gene delivery nano-device. Cationic PLA-PEG nanoparticles were prepared by nanoprecipitation method. The gene loaded nanoparticles were obtained by incubating the report gene pEGFP with cationic PLA-PEG nanoparticles. The physicochemical properties (e.g., morphology, particle size, surface charge, DNA binding efficiency) and biological properties (e.g., integrity of the released DNA, protection from nuclease degradation, plasma stability, in vitro cytotoxicity, and in vitro transfection ability in Hela cells) of the gene loaded PLA-PEG nanoparticles were evaluated, respectively. The obtained cationic PLA-PEG nanoparticles and gene loaded nanoparticles were both spherical in shape with average particle size of 89.7 and 128.9 nm, polydispersity index of 0.185 and 0.161, zeta potentials of +28.9 and +16.8 mV, respectively. The obtained cationic PLA-PEG nanoparticles with high binding efficiency (>95%) could protect the loaded DNA from the degradation by nuclease and plasma. The nanoparticles displayed sustained-release properties in vitro and the released DNA maintained its structural and functional integrity. It also showed lower cytotoxicity than Lipofectamine 2000 and could successfully transfect gene into Hela cells even in presence of serum. It could be concluded that the established gene loaded cationic PLA-PEG nanoparticles with excellent properties were promising non-viral nano-device, which had potential to make cancer gene therapy achievable

A systematic review on the effect of sweeteners on glycemic response and clinically relevant outcomes

<p>Abstract</p> <p>Background</p> <p>The major metabolic complications of obesity and type 2 diabetes may be prevented and managed with dietary modification. The use of sweeteners that provide little or no calories may help to achieve this objective.</p> <p>Methods</p> <p>We did a systematic review and network meta-analysis of the comparative effectiveness of sweetener additives using Bayesian techniques. MEDLINE, EMBASE, CENTRAL and CAB Global were searched to January 2011. Randomized trials comparing sweeteners in obese, diabetic, and healthy populations were selected. Outcomes of interest included weight change, energy intake, lipids, glycated hemoglobin, markers of insulin resistance and glycemic response. Evidence-based items potentially indicating risk of bias were assessed.</p> <p>Results</p> <p>Of 3,666 citations, we identified 53 eligible randomized controlled trials with 1,126 participants. In diabetic participants, fructose reduced 2-hour blood glucose concentrations by 4.81 mmol/L (95% CI 3.29, 6.34) compared to glucose. Two-hour blood glucose concentration data comparing hypocaloric sweeteners to sucrose or high fructose corn syrup were inconclusive. Based on two ≤10-week trials, we found that non-caloric sweeteners reduced energy intake compared to the sucrose groups by approximately 250-500 kcal/day (95% CI 153, 806). One trial found that participants in the non-caloric sweetener group had a decrease in body mass index compared to an increase in body mass index in the sucrose group (-0.40 vs 0.50 kg/m², and -1.00 vs 1.60 kg/m², respectively). No randomized controlled trials showed that high fructose corn syrup or fructose increased levels of cholesterol relative to other sweeteners.</p> <p>Conclusions</p> <p>Considering the public health importance of obesity and its consequences; the clearly relevant role of diet in the pathogenesis and maintenance of obesity; and the billions of dollars spent on non-caloric sweeteners, little high-quality clinical research has been done. Studies are needed to determine the role of hypocaloric sweeteners in a wider population health strategy to prevent, reduce and manage obesity and its consequences.</p

Angiogenesis and chronic kidney disease

The number of patients requiring renal replacement therapy due to end-stage renal disease (ESRD) is increasing worldwide. The prevalence of chronic kidney disease (CKD), and the importance of CKD as a risk factor in development of ESRD and in complicating cardiovascular disease (CVD) have been confirmed. In recent years, the involvement of angiogenesis-related factors in the progression of CKD has been studied, and the potential therapeutic effects on CKD of modulating these factors have been identified. Vascular endothelial growth factor (VEGF)-A, a potent pro-angiogenic factor, is involved in the development of the kidney, in maintenance of the glomerular capillary structure and filtration barrier, and in the renal repair process after injury. VEGF-A is also involved in the development of early diabetic nephropathy, demonstrated by the therapeutic effects of anti-VEGF-A antibody. Angiopoietin (Ang)-1 induces the maturation of newly formed blood vessels, and the therapeutic effects of Ang-1 in diabetic nephropathy have been described. In experimental models of diabetic nephropathy, the therapeutic effects of angiogenesis inhibitors, including angiostatin, endostatin and tumstatin peptides, the isocoumarin NM-3, and vasohibin-1, have been reported

Linseed oil-based concrete surface treatment for building and highway structures in Hong Kong

This experimental program investigated the effectiveness of concrete surface treatment using four Canadian linseed oil-based sealants on concrete specimens made from G30/20 and G45/20 concretes, which are typically used for building and highway structures in Hong Kong. The tests were conducted at the University of Hong Kong and the University of Manitoba independently, using samples cast from the same mixes in Hong Kong. A total of more than 500 specimens were tested in each university for salt spray resistance, carbonation, bond strength, dripping and ultra-violet weathering. The results show that the four sealants were capable of penetrating through vertical, upward horizontal and downward horizontal concrete surfaces up to depths of 2.5 mm. All the four sealants significantly enhanced the resistance of G30/20 concrete against salt spray attack and carbonation. The resistance of G45/20 concrete to salt spray was also increased. Up to a duration limit of 500 hours of quick ultra-violet weathering, the treated specimens still demonstrated higher resistance against salt spray attack and carbonation than the untreated ones. However, no conclusion can be made regarding the effect on carbonation resistance of G45/20 concrete, since both the treated and untreated specimens showed no signs of carbonation after the test.link_to_subscribed_fulltex

Development of statistical design models for concrete sandwich panels with continuous glass-fiber-reinforced polymer shear connectors

This article proposes a statistical framework for the development of design models for concrete sandwich panels with glass-fiber-reinforced polymer shear grids. The framework is developed by integrating the Bayesian parameter estimation method and the Eurocode-based capacity reduction factor calibration method. In the first part of the framework, probabilistic and deterministic shear flow prediction models are proposed based on 32 experimental data. It is seen that the contribution of glass-fiber-reinforced polymer grids is dominant, although parameters on the geometrical and material properties of insulation and concrete wythes also contribute. Different constant terms for bias correction of the proposed models are proposed according to the insulation type, and the prediction error of the developed model was reduced. In the second part of the framework, the capacity reduction factor for the proposed deterministic formulas is calculated for design purposes. Statistical calibrations for capacity factors are carried out to meet a target reliability level, and the value is estimated to be approximately 0.75 for all proposed models. Further data collection will improve the applicability of the proposed models and clarify quantification of the contribution of parameters