Seed systems smallholder farmers use

Seed can be an important entry point for promoting productivity, nutrition and resilience among smallholder farmers. While investments have primarily focused on strengthening the formal sector, this article documents the degree to which the informal sector remains the core for seed acquisition, especially in Africa. Conclusions drawn from a uniquely comprehensive data set, 9660 observations across six countries and covering 40 crops, show that farmers access 90.2 % of their seed from informal systems with 50.9 % of that deriving from local markets. Further, 55 % of seed is paid for by cash, indicating that smallholders are already making important investments in this arena. Targeted interventions are proposed for rendering formal and informal seed sector more smallholder-responsive and for scaling up positive impacts

Properties of odd nuclei and the impact of time-odd mean fields: A systematic Skyrme-Hartree-Fock analysis

We present a systematic analysis of the description of odd nuclei by the Skyrme-Hartree-Fock approach augmented with pairing in BCS approximation and blocking of the odd nucleon. Current and spin densities in the Skyrme functional produce time-odd mean fields (TOMF) for odd nuclei. Their effect on basic properties (binding energies, odd-even staggering, separation energies and spectra) is investigated for the three Skyrme parameterizations SkI3, SLy6, and SV-bas. About 1300 spherical and axially-deformed odd nuclei with 16 < Z < 92 are considered. The calculations demonstrate that the TOMF effect is generally small, although not fully negligible. The influence of the Skyrme parameterization and the consistency of the calculations are much more important. With a proper choice of the parameterization, a good description of binding energies and their differences is obtained, comparable to that for even nuclei. The description of low-energy excitation spectra of odd nuclei is of varying quality depending on the nucleus

New results of 116Cd double beta decay study with 116CdWO4 scintillators

A new phase of 116Cd double beta decay experiment is in progress in the Solotvina Underground Laboratory. Four enriched 116CdWO4 scintillators with total mass 339 g are used in a set up, whose active shield is made of 15 natural CdWO4 crystals (20.6 kg). The background rate in the energy interval 2.5-3.2 MeV is 0.03 counts/y*kg*keV. The half-life for 2-neutrino 2-beta decay of 116Cd is measured as T{1/2}(2-neutrino) = [2.6+-0.1(stat)-0.4+0.7}(syst)]*10**19 y. The T{1/2} limits for neutrinoless 2-beta decay of 116Cd are set as T{1/2} >= 0.7(2.5)*10**23 y at 90%(68%) C.L. for transition to ground state of 116Sn, while for decays to the first 2+ and second 0+ excited levels of 116Sn as T{1/2}>=1.3(4.8)*10**22 y and >=0.7(2.4)*10**22 y with 90%(68%) C.L., respectively. For 0-neutrino 2-beta decay with emission of one or two Majorons, the limits are T{1/2}(0-neutrino M1) >=3.7(5.8)*10**21 y and T{1/2}(0-neutrino M2)>=5.9(9.4)*10**20 y at 90%(68%) C.L. Restrictions on the value of the neutrino mass, right-handed admixtures in the weak interaction, and the neutrino-Majoron coupling constant are derived as: m(neutrino)<=2.6(1.4) eV, eta <=3.9*10**-8, lambda <=3.4*10**-6, and g{M}<= 12(9.5)*10**-5 at 90%(68%) C.L., respectively.Comment: 28 pages, 9 figures (LaTeX). Phys. Rev. C (in press

NQO1-Dependent Redox Cycling of Idebenone: Effects on Cellular Redox Potential and Energy Levels

Short-chain quinones are described as potent antioxidants and in the case of idebenone have already been under clinical investigation for the treatment of neuromuscular disorders. Due to their analogy to coenzyme Q10 (CoQ10), a long-chain quinone, they are widely regarded as a substitute for CoQ10. However, apart from their antioxidant function, this provides no clear rationale for their use in disorders with normal CoQ10 levels. Using recombinant NAD(P)H:quinone oxidoreductase (NQO) enzymes, we observed that contrary to CoQ10 short-chain quinones such as idebenone are good substrates for both NQO1 and NQO2. Furthermore, the reduction of short-chain quinones by NQOs enabled an antimycin A-sensitive transfer of electrons from cytosolic NAD(P)H to the mitochondrial respiratory chain in both human hepatoma cells (HepG2) and freshly isolated mouse hepatocytes. Consistent with the substrate selectivity of NQOs, both idebenone and CoQ1, but not CoQ10, partially restored cellular ATP levels under conditions of impaired complex I function. The observed cytosolic-mitochondrial shuttling of idebenone and CoQ1 was also associated with reduced lactate production by cybrid cells from mitochondrial encephalomyopathy, lactic acidosis and stroke-like episodes (MELAS) patients. Thus, the observed activities separate the effectiveness of short-chain quinones from the related long-chain CoQ10 and provide the rationale for the use of short-chain quinones such as idebenone for the treatment of mitochondrial disorders

Unexpected mode of engagement between enterovirus 71 and its receptor SCARB2

Enterovirus 71 (EV71) is a common cause of hand, foot and mouth disease—a disease endemic especially in the Asia-Pacific region1. Scavenger receptor class B member 2 (SCARB2) is the major receptor of EV71, as well as several other enteroviruses responsible for hand, foot and mouth disease, and plays a key role in cell entry2. The isolated structures of EV71 and SCARB2 are known3,4,5,6, but how they interact to initiate infection is not. Here, we report the EV71–SCARB2 complex structure determined at 3.4 Å resolution using cryo-electron microscopy. This reveals that SCARB2 binds EV71 on the southern rim of the canyon, rather than across the canyon, as predicted3,7,8. Helices 152–163 (α5) and 183–193 (α7) of SCARB2 and the viral protein 1 (VP1) GH and VP2 EF loops of EV71 dominate the interaction, suggesting an allosteric mechanism by which receptor binding might facilitate the low-pH uncoating of the virus in the endosome/lysosome. Remarkably, many residues within the binding footprint are not conserved across SCARB2-dependent enteroviruses; however, a conserved proline and glycine seem to be key residues. Thus, although the virus maintains antigenic variability even within the receptor-binding footprint, the identification of binding ‘hot spots’ may facilitate the design of receptor mimic therapeutics less likely to quickly generate resistance