442 research outputs found
Modern views of ancient metabolic networks
Metabolism is a molecular, cellular, ecological and planetary phenomenon, whose fundamental principles are likely at the heart of what makes living matter different from inanimate one. Systems biology approaches developed for the quantitative analysis of metabolism at multiple scales can help understand metabolism's ancient history. In this review, we highlight work that uses network-level approaches to shed light on key innovations in ancient life, including the emergence of proto-metabolic networks, collective autocatalysis and bioenergetics coupling. Recent experiments and computational analyses have revealed new aspects of this ancient history, paving the way for the use of large datasets to further improve our understanding of life's principles and abiogenesis.https://www.sciencedirect.com/science/article/pii/S2452310017302196Published versio
Metabolic network percolation quantifies biosynthetic capabilities across the human oral microbiome
The biosynthetic capabilities of microbes underlie their growth and interactions, playing a prominent role in microbial community structure. For large, diverse microbial communities, prediction of these capabilities is limited by uncertainty about metabolic functions and environmental conditions. To address this challenge, we propose a probabilistic method, inspired by percolation theory, to computationally quantify how robustly a genome-derived metabolic network produces a given set of metabolites under an ensemble of variable environments. We used this method to compile an atlas of predicted biosynthetic capabilities for 97 metabolites across 456 human oral microbes. This atlas captures taxonomically-related trends in biomass composition, and makes it possible to estimate inter-microbial metabolic distances that correlate with microbial co-occurrences. We also found a distinct cluster of fastidious/uncultivated taxa, including several Saccharibacteria (TM7) species, characterized by their abundant metabolic deficiencies. By embracing uncertainty, our approach can be broadly applied to understanding metabolic interactions in complex microbial ecosystems.T32GM008764 - NIGMS NIH HHS; T32 GM008764 - NIGMS NIH HHS; R01 DE024468 - NIDCR NIH HHS; R01 GM121950 - NIGMS NIH HHS; DE-SC0012627 - Biological and Environmental Research; RGP0020/2016 - Human Frontier Science Program; NSFOCE-BSF 1635070 - National Science Foundation; HR0011-15-C-0091 - Defense Advanced Research Projects Agency; R37DE016937 - NIDCR NIH HHS; R37 DE016937 - NIDCR NIH HHS; R01GM121950 - NIGMS NIH HHS; R01DE024468 - NIDCR NIH HHS; 1457695 - National Science FoundationPublished versio
Environmental boundary conditions for the origin of life converge to an organo-sulfur metabolism
Published in final edited form as:
Nat Ecol Evol. 2019 December ; 3(12): 1715–1724. doi:10.1038/s41559-019-1018-8.It has been suggested that a deep memory of early life is hidden in the architecture of metabolic networks, whose reactions could have been catalyzed by small molecules or minerals before genetically encoded enzymes. A major challenge in unravelling these early steps is assessing the plausibility of a connected, thermodynamically consistent proto-metabolism under different geochemical conditions, which are still surrounded by high uncertainty. Here we combine network-based algorithms with physico-chemical constraints on chemical reaction networks to systematically show how different combinations of parameters (temperature, pH, redox potential and availability of molecular precursors) could have affected the evolution of a proto-metabolism. Our analysis of possible trajectories indicates that a subset of boundary conditions converges to an organo-sulfur-based proto-metabolic network fuelled by a thioester- and redox-driven variant of the reductive tricarboxylic acid cycle that is capable of producing lipids and keto acids. Surprisingly, environmental sources of fixed nitrogen and low-potential electron donors are not necessary for the earliest phases of biochemical evolution. We use one of these networks to build a steady-state dynamical metabolic model of a protocell, and find that different combinations of carbon sources and electron donors can support the continuous production of a minimal ancient 'biomass' composed of putative early biopolymers and fatty acids.80NSSC17K0295 - Intramural NASA; 80NSSC17K0296 - Intramural NASA; T32 GM100842 - NIGMS NIH HHSAccepted manuscrip
Remnants of an ancient metabolism without phosphate
Phosphate is essential for all living systems, serving as a building block of genetic and metabolic machinery. However, it is unclear how phosphate could have assumed these central roles on primordial Earth, given its poor geochemical accessibility. We used systems biology approaches to explore the alternative hypothesis that a protometabolism could have emerged prior to the incorporation of phosphate. Surprisingly, we identified a cryptic phosphate-independent core metabolism producible from simple prebiotic compounds. This network is predicted to support the biosynthesis of a broad category of key biomolecules. Its enrichment for enzymes utilizing iron-sulfur clusters, and the fact that thermodynamic bottlenecks are more readily overcome by thioester rather than phosphate couplings, suggest that this network may constitute a "metabolic fossil" of an early phosphate-free nonenzymatic biochemistry. Our results corroborate and expand previous proposals that a putative thioester-based metabolism could have predated the incorporation of phosphate and an RNA-based genetic system. PAPERCLIP
The Dynamics of Hybrid Metabolic-Genetic Oscillators
The synthetic construction of intracellular circuits is frequently hindered
by a poor knowledge of appropriate kinetics and precise rate parameters. Here,
we use generalized modeling (GM) to study the dynamical behavior of topological
models of a family of hybrid metabolic-genetic circuits known as
"metabolators." Under mild assumptions on the kinetics, we use GM to
analytically prove that all explicit kinetic models which are topologically
analogous to one such circuit, the "core metabolator," cannot undergo Hopf
bifurcations. Then, we examine more detailed models of the metabolator.
Inspired by the experimental observation of a Hopf bifurcation in a
synthetically constructed circuit related to the core metabolator, we apply GM
to identify the critical components of the synthetically constructed
metabolator which must be reintroduced in order to recover the Hopf
bifurcation. Next, we study the dynamics of a re-wired version of the core
metabolator, dubbed the "reverse" metabolator, and show that it exhibits a
substantially richer set of dynamical behaviors, including both local and
global oscillations. Prompted by the observation of relaxation oscillations in
the reverse metabolator, we study the role that a separation of genetic and
metabolic time scales may play in its dynamics, and find that widely separated
time scales promote stability in the circuit. Our results illustrate a generic
pipeline for vetting the potential success of a potential circuit design,
simply by studying the dynamics of the corresponding generalized model
Genome-scale architecture of small molecule regulatory networks and the fundamental trade-off between regulation and enzymatic activity
Metabolic flux is in part regulated by endogenous small molecules that modulate the catalytic activity of an enzyme, e.g., allosteric inhibition. In contrast to transcriptional regulation of enzymes, technical limitations have hindered the production of a genome-scale atlas of small molecule-enzyme regulatory interactions. Here, we develop a framework leveraging the vast, but fragmented, biochemical literature to reconstruct and analyze the small molecule regulatory network (SMRN) of the model organism Escherichia coli, including the primary metabolite regulators and enzyme targets. Using metabolic control analysis, we prove a fundamental trade-off between regulation and enzymatic activity, and we combine it with metabolomic measurements and the SMRN to make inferences on the sensitivity of enzymes to their regulators. Generalizing the analysis to other organisms, we identify highly conserved regulatory interactions across evolutionarily divergent species, further emphasizing a critical role for small molecule interactions in the maintenance of metabolic homeostasis.P30 CA008748 - NCI NIH HHS; R01 GM121950 - NIGMS NIH HH
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Resource Competition May Lead to Effective Treatment of Antibiotic Resistant Infections
Drug resistance is a common problem in the fight against infectious diseases. Recent studies have shown conditions (which we call antiR) that select against resistant strains. However, no specific drug administration strategies based on this property exist yet. Here, we mathematically compare growth of resistant versus sensitive strains under different treatments (no drugs, antibiotic, and antiR), and show how a precisely timed combination of treatments may help defeat resistant strains. Our analysis is based on a previously developed model of infection and immunity in which a costly plasmid confers antibiotic resistance. As expected, antibiotic treatment increases the frequency of the resistant strain, while the plasmid cost causes a reduction of resistance in the absence of antibiotic selection. Our analysis suggests that this reduction occurs under competition for limited resources. Based on this model, we estimate treatment schedules that would lead to a complete elimination of both sensitive and resistant strains. In particular, we derive an analytical expression for the rate of resistance loss, and hence for the time necessary to turn a resistant infection into sensitive (tclear). This time depends on the experimentally measurable rates of pathogen division, growth and plasmid loss. Finally, we estimated tclear for a specific case, using available empirical data, and found that resistance may be lost up to 15 times faster under antiR treatment when compared to a no treatment regime. This strategy may be particularly suitable to treat chronic infection. Finally, our analysis suggests that accounting explicitly for a resistance-decaying rate may drastically change predicted outcomes in host-population models
Thermodynamics drives coenzyme redundancy in metabolism
Coenzymes redistribute a variety of resources (e.g., electrons, phosphate groups, methyl groups) throughout cellular metabolism. For a variety of reactions requiring acceptors or donors of specific resources, there often exist degenerate sets of molecules (e.g., NAD(H) and NADP(H)) that carry out similar functions. Several hypotheses can explain the persistence of coenzyme degeneracy, but none have been tested quantitatively. Here, we use genome-wide metabolic modeling approaches to decompose the selective pressures driving enzymatic specificity for coenzymes in the metabolic network of Escherichia coli. Flux balance modeling predicts that only two enzymes (encoded by leuB and pdxB) are thermodynamically constrained to use NAD(H) over NADP(H). In contrast, structural and sequence analyses reveal widespread conservation of residues that retain selectivity for NAD(H), suggesting that additional forces drive enzyme specificity. Using a model accounting for the cost of oxidoreductase enzyme expression, we find that coenzyme redundancy universally reduces the minimal amount of protein required to catalyze coenzyme-coupled reactions, inducing individual reactions to strongly prefer one coenzyme over another when reactions are near thermodynamic equilibrium. We propose that partitioning of flux across multiple coenzyme pools could be a generic phenomenon of cellular metabolism, and hypothesize that coenzymes typically thought to exist in a single pool (e.g., CoA) may exist in more than one form (e.g., dephospho-CoA)
Majorana and the quasi-stationary states in Nuclear Physics
A complete theoretical model describing artificial disintegration of nuclei
by bombardment with alpha-particles, developed by Majorana as early as in 1930,
is discussed in detail alongside the basic experimental evidences that
motivated it. By following the quantum dynamics of a state resulting from the
superposition of a discrete state with a continuum one, whose interaction is
described by a given potential term, Majorana obtained (among the other
predictions) the explicit expression for the integrated cross section of the
nuclear process, which is the direct measurable quantity of interest in the
experiments. Though this is the first application of the concept of
quasi-stationary states to a Nuclear Physics problem, it seems also that the
unpublished Majorana's work anticipates by several years the related seminal
paper by Fano on Atomic Physics.Comment: latex, amsart, 13 page
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