25 research outputs found

    Regulation of cell cycle-specific gene expression in fission yeast by the Cdc14p-like phosphatase Clp1p

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    Regulated gene expression makes an important contribution to cell cycle control mechanisms. In fission yeast, a group of genes is coordinately expressed during a late stage of the cell cycle (M phase and cytokinesis) that is controlled by common cis-acting promoter motifs named pombe cell cycle boxes (PCBs), which are bound by a trans-acting transcription factor complex, PCB binding factor (PBF). PBF contains at least three transcription factors, a MADS box protein Mbx1p and two forkhead transcription factors, Sep1p and Fkh2p. Here we show that the fission yeast Cdc14p-like phosphatase Clp1p (Flp1p) controls M–G1 specific gene expression through PBF. Clp1p binds in vivo both to Mbx1p, a MADS box-like transcription factor, and to the promoters of genes transcribed at this cell cycle time. Because Clp1p dephosphorylates Mbx1p in vitro, and is required for Mbx1p cell cycle-specific dephosphorylation in vivo, our observations suggest that Clp1p controls cell cycle-specific gene expression through binding to and dephosphorylating Mbx1p

    InfluĂȘncia da temperatura corporal de cascavĂ©is (Crotalus durissus) submetidas Ă  anestesia com cetamina Influence of body temperature on rattlesnakes (Crotalus durissus) anesthetized with ketamine

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    O estudo objetivou verificar a influĂȘncia da temperatura corporal nos parĂąmetros fisiolĂłgicos e nos perĂ­odos de indução e recuperação anestĂ©sicos de cascavĂ©is (Crotalus durissus) anestesiadas com cetamina. Os animais foram previamente submetidos Ă  hipotermia (HIPO) (<22°C) e normotermia (30°C) (NORMO) e anestesiados com 80mg/kg IM de cetamina. Foram avaliados os perĂ­odos de latĂȘncia e recuperação da anestesia por meio do tĂŽnus de cabeça, tĂŽnus muscular e reflexo de endireitamento. Mensurou-se a frequĂȘncia cardĂ­aca (FC), tempo de apnĂ©ia e temperatura corporal em 0 min e 5, 10, 15, 30, 60, 90, 120 min e anĂĄlise dos gases sanguĂ­neos em 0 min, 30 e 60 min. NĂŁo houve diferença em relação ao perĂ­odo de latĂȘncia entre os grupos. A recuperação dos animais em HIPO foi mais prolongada (5,5 horas) que em NORMO (3,5 horas). Obteve-se FC no grupo NORMO superior que no grupo HIPO. O tempo de apnĂ©ia manteve o mesmo padrĂŁo em ambos os grupos. Em relação ao basal, tanto em HIPO quanto em NORMO o tempo de apnĂ©ia diminuiu acentuadamente entre 5 e 30 min. Observou-se acidose respiratĂłria no grupo NORMO apenas em 0 min. O SvO2 elevou-se significativamente apĂłs 30 min, o mesmo ocorrendo com a PvO2. A PvCO2 diminuiu em ambos os grupos apĂłs 30 min. Evidenciou-se que a temperatura corporal influencia intrinsecamente o perĂ­odo de recuperação de cascavĂ©is anestesiadas com cetamina.<br>The aim of the study was to verify the influence of the body temperature under physiological values and latency and recovery times on rattlesnakes anesthetized with ketamine. The animals were previously submitted to hypothermia (HYPO) (<22°C) and normothermia (30°C) (NORMO) and then, anesthetized with 80 mg/kg IM of ketamine. Latency and recovery times were evaluated by head tonus, muscular tonus and righting reflex. Heart rate (HR), time of apnea and body temperature were measured before and 5, 10, 15, 30, 60, 90 and 120 minutes after ketamine administration. Blood gases parameters were measured before, 30 and 60 minutes. It was not observed difference on latency time in both groups. The recovery time was higher on HYPO (5,5 hours) compared to NORMO (3,5 hours). HR was higher on NORMO compared to HYPO. Time of apnea was the same pattern on both groups. Compared to basal levels, time of apnea was shorter between 5 to 30 min on both groups. Respiratory acidosis was observed only at 0 min in NORMO. SvO2 was higher after 30 min, the same as with PvO2 in both groups. PvCO2 reduced after 30 min in both groups. It was evident that body temperature exerts intense influence on the recovery time on rattlesnakes anesthetized with ketamine
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