5,328 research outputs found
Microlensing Characterization of Wide-Separation Planets
With their excellent photometric precision and dramatic increase in
monitoring frequency, future microlensing survey experiments are expected to be
sensitive to very short time-scale, isolated events caused by free-floating and
wide-separation planets with mass as low as a few lunar masses. We estimate the
probability of measuring the Einstein radius \theta_E for bound and
free-floating planets. We carry out detailed simulations of the planetary
events expected in next-generation surveys and estimate the resulting
uncertainty in \theta_E for these events. We show that, for main-sequence
sources and Jupiter-mass planets, the caustic structure of wide-separation
planets with projected separations of < 20 AU substantially increases the
probability of measuring the dimensionless source size and thus determining
\theta_E compared to the case of unbound planets. In this limit where the
source is much smaller than the caustic, the effective cross-section to measure
\theta_E to 10% is ~25% larger than the full width of the caustic. Measurement
of the lens parallax is possible for low-mass planetary events by combined
observations from the ground and a satellite located in an L2 orbit; this would
complete the mass measurements for such wide-separation planets. Finally,
short-duration events caused by bound planets can be routinely distinguished
from those caused by free-floating planets for planet-star separations < 20 AU
from either the deviations due to the planetary caustic or (more often) the
low-amplitude bump from the magnification due to the parent star.Comment: 10 pages including 7 figures. ApJ, in pres
Biochemical Characterization of Anopheles gambiae SRPN6, a Malaria Parasite Invasion Marker in Mosquitoes
Serine proteinase inhibitors of the serpin family are well known as negative regulators of hemostasis, thrombolysis and innate immune responses. Additionally, non-inhibitory serpins serve functions as chaperones, hormone transporters, or anti-angiogenic factors. In the African malaria mosquito, Anopheles gambiae s.s., at least three serpins (SRPNs) are implicated in the innate immune response against malaria parasites. Based on reverse genetic and cell biological analyses, AgSRPN6 limits parasite numbers and transmission and has been postulated to control melanization and complement function in mosquitoes. This study aimed to characterize AgSRPN6 biophysically and determine its biochemical mode of action. The structure model of AgSRPN6, as predicted by I-Tasser showed the protein in the native serpin fold, with three central β-sheets, nine surrounding α-helices, and a protruding reactive center loop. This structure is in agreement with biophysical and functional data obtained from recombinant (r) AgSRPN6, produced in Escherichia coli. The physical properties of purified rAgSRPN6 were investigated by means of analytical ultracentrifugation, circular dichroism, and differential scanning calorimetry tools. The recombinant protein exists predominantly as a monomer in solution, is composed of a mixture of α-helices and β-sheets, and has a mid-point unfolding temperature of 56°C. Recombinant AgSRPN6 strongly inhibited porcine pancreatic kallikrein and to a lesser extent bovine pancreatic trypsin in vitro. Furthermore, rAgSRPN6 formed inhibitory, SDS-stable, higher molecular weight complexes with prophenoloxidase-activating proteinase (PAP)1, PAP3, and Hemolymph protein (HP)6, which are required for melanization in the lepidopteran model organism, Manduca sexta. Taken together, our results strongly suggest that AgSRPN6 takes on a native serpin fold and is an inhibitor of trypsin-like serine proteinases.This work was supported by the National Institutes of Health through 3P20RR017708-07S1 and P20RR017686 sub-awards and 1R01AI095842 to K.M. This is contribution 12-098-J from the Kansas Agricultural Experiment Station
IR Spectrum of the O-HO Hydrogen Bond of Phthalic Acid Monomethylester in Gas Phase and in CCl Solution
The absorption spectrum of the title compound in the spectral range of the
Hydrogen-bonded OH-stretching vibration has been investigated using a
five-dimensional gas phase model as well as a QM/MM classical molecular
dynamics simulation in solution. The gas phase model predicts a Fermi-resonance
between the OH-stretching fundamental and the first OH-bending overtone
transition with considerable oscillator strength redistribution. The anharmonic
coupling to a low-frequency vibration of the Hydrogen bond leading to a
vibrational progression is studied within a diabatic potential energy curve
model. The condensed phase simulation of the dipole-dipole correlation function
results in a broad band in the 3000 \cm region in good agreement with
experimental data. Further, weaker absorption features around 2600 \cm have
been identified as being due to motion of the Hydrogen within the Hydrogen
bond.Comment: Contribution to Horizons in Hydrogen Bond Research Conference, Paris
200
LIN28B and Let-7 in Diffuse Midline Glioma: A Review
Diffuse midline glioma (DMG) is the most lethal of all childhood cancers. DMGs are driven by histone-tail-mutation-mediated epigenetic dysregulation and partner mutations in genes controlling proliferation and migration. One result of this epigenetic and genetic landscape is the overexpression of LIN28B RNA binding protein. In other systems, LIN28B has been shown to prevent let-7 microRNA biogenesis; however, let-7, when available, faithfully suppresses tumorigenic pathways and induces cellular maturation by preventing the translation of numerous oncogenes. Here, we review the current literature on LIN28A/B and the let-7 family and describe their role in gliomagenesis. Future research is then recommended, with a focus on the mechanisms of LIN28B overexpression and localization in DMG
Genetic Correction of Huntington's Disease Phenotypes in Induced Pluripotent Stem Cells
SummaryHuntington's disease (HD) is caused by a CAG expansion in the huntingtin gene. Expansion of the polyglutamine tract in the huntingtin protein results in massive cell death in the striatum of HD patients. We report that human induced pluripotent stem cells (iPSCs) derived from HD patient fibroblasts can be corrected by the replacement of the expanded CAG repeat with a normal repeat using homologous recombination, and that the correction persists in iPSC differentiation into DARPP-32-positive neurons in vitro and in vivo. Further, correction of the HD-iPSCs normalized pathogenic HD signaling pathways (cadherin, TGF-β, BDNF, and caspase activation) and reversed disease phenotypes such as susceptibility to cell death and altered mitochondrial bioenergetics in neural stem cells. The ability to make patient-specific, genetically corrected iPSCs from HD patients will provide relevant disease models in identical genetic backgrounds and is a critical step for the eventual use of these cells in cell replacement therapy
Comparison of the CDC Backpack aspirator and the Prokopack aspirator for sampling indoor- and outdoor-resting mosquitoes in southern Tanzania.
BACKGROUND\ud
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Resting mosquitoes can easily be collected using an aspirating device. The most commonly used mechanical aspirator is the CDC Backpack aspirator. Recently, a simple, and low-cost aspirator called the Prokopack has been devised and proved to have comparable performance. The following study evaluates the Prokopack aspirator compared to the CDC backpack aspirator when sampling resting mosquitoes in rural Tanzania.\ud
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METHODS\ud
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Mosquitoes were sampled in- and outdoors of 48 typical rural African households using both aspirators. The aspirators were rotated between collectors and households in a randomized, Latin Square design. Outdoor collections were performed using artificial resting places (large barrel and car tyre), underneath the outdoor kitchen (kibanda) roof and from a drop-net. Data were analysed with generalized linear models.\ud
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RESULTS\ud
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The number of mosquitoes collected using the CDC Backpack and the Prokopack aspirator were not significantly different both in- and outdoors (indoors p = 0.735; large barrel p = 0.867; car tyre p = 0.418; kibanda p = 0.519). The Prokopack was superior for sampling of drop-nets due to its smaller size. The number mosquitoes collected per technician was more consistent when using the Prokopack aspirator. The Prokopack was more user-friendly: technicians preferred using the it over the CDC backpack aspirator as it weighs considerably less, retains its charge for longer and is easier to manoeuvre.\ud
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CONCLUSIONS\ud
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The Prokopack proved in the field to be more advantageous than the CDC Backpack aspirator. It can be self assembled using simple, low-cost and easily attainable materials. This device is a useful tool for researchers or vector-control surveillance programs operating in rural Africa, as it is far simpler and quicker than traditional means of sampling resting mosquitoes. Further longitudinal evaluations of the Prokopack aspirator versus the gold standard pyrethrum spray catch for indoor resting catches are recommended
Non-Deterministic Kleene Coalgebras
In this paper, we present a systematic way of deriving (1) languages of
(generalised) regular expressions, and (2) sound and complete axiomatizations
thereof, for a wide variety of systems. This generalizes both the results of
Kleene (on regular languages and deterministic finite automata) and Milner (on
regular behaviours and finite labelled transition systems), and includes many
other systems such as Mealy and Moore machines
Nonlinear thermoelectric response of quantum dots: renormalized dual fermions out of equilibrium
The thermoelectric transport properties of nanostructured devices continue to
attract attention from theorists and experimentalist alike as the spatial
confinement allows for a controlled approach to transport properties of
correlated matter. Most of the existing work, however, focuses on
thermoelectric transport in the linear regime despite the fact that the
nonlinear conductance of correlated quantum dots has been studied in some
detail throughout the last decade. Here, we review our recent work on the
effect of particle-hole asymmetry on the nonlinear transport properties in the
vicinity of the strong coupling limit of Kondo-correlated quantum dots and
extend the underlying method, a renormalized superperturbation theory on the
Keldysh contour, to the thermal conductance in the nonlinear regime. We
determine the charge, energy, and heat current through the nanostructure and
study the nonlinear transport coefficients, the entropy production, and the
fate of the Wiedemann-Franz law in the non-thermal steady-state. Our approach
is based on a renormalized perturbation theory in terms of dual fermions around
the particle-hole symmetric strong-coupling limit.Comment: chapter contributed to 'New Materials for Thermoelectric
Applications: Theory and Experiment' Springer Series: NATO Science for Peace
and Security Series - B: Physics and Biophysics, Veljko Zlatic (Editor), Alex
Hewson (Editor). ISBN: 978-9400749863 (2012
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