446 research outputs found
Validity and Reliability of Self-reported Diabetes in the Atherosclerosis Risk in Communities Study
The objective of this study was to assess the validity of prevalent and incident self-reported diabetes compared with multiple reference definitions and to assess the reliability (repeatability) of a self-reported diagnosis of diabetes. Data from 10,321 participants in the Atherosclerosis Risk in Communities (ARIC) Study who attended visit 4 (1996–1998) were analyzed. Prevalent self-reported diabetes was compared with reference definitions defined by fasting glucose and medication use obtained at visit 4. Incident self-reported diabetes was assessed during annual follow-up telephone calls and was compared with reference definitions defined by fasting glucose, hemoglobin A1c, and medication use obtained during an in-person visit attended by a subsample of participants (n = 1,738) in 2004–2005. The sensitivity of prevalent self-reported diabetes ranged from 58.5% to 70.8%, and specificity ranged from 95.6% to 96.8%, depending on the reference definition. Similarly, the sensitivity of incident self-reported diabetes ranged from 55.9% to 80.4%, and specificity ranged from 84.5% to 90.6%. Percent positive agreement of self-reported diabetes during 9 years of repeat assessments ranged from 92.7% to 95.4%. Both prevalent self-reported diabetes and incident self-reported diabetes were 84%–97% specific and 55%–80% sensitive as compared with reference definitions using glucose and medication criteria. Self-reported diabetes was >92% reliable over time
The effects of conjugated linoleic acid isomers cis-9,trans-11 and trans-10,cis-12 on in vitro bovine embryo production and cryopreservation
Conjugated linoleic acid (CLA) isomers can affect the lipid profile and signaling of cells and thereby alter their function. A total of 5,700 bovine oocytes were used in a structured series of experiments to test the effects of CLA cis-9,trans-11 and CLA trans-10,cis-12 in vitro. In experiment 1, high doses of each CLA isomer during in vitro maturation (IVM) were compared with high or low doses during the entire in vitro culture (IVC) of parthenogenetic embryos. High doses of the CLA isomers ranged from 50 to 200 μM and low doses were 15 and 25 μM. In experiment 2, the low doses of each CLA isomer were tested during IVM/IVC on embryos produced by in vitro fertilization (IVF). Experiment 3 compared the effects of 15 μM doses of each CLA isomer during IVM or IVC of IVF embryos. In experiment 4, post-rewarming survival rates and blastomere counts were assessed for embryos supplemented with each CLA isomer during IVM or for 36 h before vitrification. In experiment 1, when either CLA isomer was provided only during IVM, we observed no effects on overall rates of maturation, cleavage, or blastocysts (92.2 ± 1.6%, 78.3 ± 4.1%, and 28.9 ± 5.1%, respectively). However, high doses of each CLA isomer, but not low doses, during the entire embryo culture period decreased blastocyst rates (5-20%) in a dose-dependent manner. Cleavage rates improved with 15 or 50 μM CLA trans-10,cis-12. Progesterone concentrations in maturation media were significantly increased by high doses of each CLA isomer compared with control, but low doses of CLA isomers had no effect. In experiment 2 with IVF embryos, low doses of each CLA isomer did not alter cleavage rates (average 84.9 ± 1.9%) and only 25 μM CLA trans-10,cis-12 during IVC reduced blastocyst rates below those of controls (25.5 ± 2.1 vs. 38.2 ± 2.3%). The lipid content of embryos was increased and relative expression of the BIRC5 (baculoviral IAP repeat containing 5) gene was depressed by CLA trans-10,cis-12. In experiment 3, low doses (15μM) of each CLA isomer during IVC significantly reduced blastocyst rates (20.6 ± 2.4% and 27.7 ± 1.2% vs. 34.18 ± 1.2% for CLA trans-10,cis-12 and CLA cis-9,trans-11 compared with control, respectively) with less effect of each CLA during IVM. In experiment 4, adding 100 μM CLA cis-9,trans-11 during the final 36 h of culture resulted in a high survival rate after rewarming and culture, and the higher embryo blastomere count was comparable to that of control embryos not undergoing vitrification. In conclusion, supplementation with either CLA isomer did not improve embryo production, but inclusion of CLA cis-9,trans-11 before vitrification improved the quality of bovine IVF embryos after rewarming and culture
Accurate Atmospheric Parameters at Moderate Resolution Using Spectral Indices: Preliminary Application to the MARVELS Survey
Studies of Galactic chemical and dynamical evolution in the solar
neighborhood depend on the availability of precise atmospheric parameters
(Teff, [Fe/H] and log g) for solar-type stars. Many large-scale spectroscopic
surveys operate at low to moderate spectral resolution for efficiency in
observing large samples, which makes the stellar characterization difficult due
to the high degree of blending of spectral features. While most surveys use
spectral synthesis, in this work we employ an alternative method based on
spectral indices to determine the atmospheric parameters of a sample of nearby
FGK dwarfs and subgiants observed by the MARVELS survey at moderate resolving
power (R~12,000). We have developed three codes to automatically normalize the
observed spectra, measure the equivalent widths of the indices and, through the
comparison of those with values calculated with pre-determined calibrations,
derive the atmospheric parameters of the stars. The calibrations were built
using a sample of 309 stars with precise stellar parameters obtained from the
analysis of high-resolution FEROS spectra. A validation test of the method was
conducted with a sample of 30 MARVELS targets that also have reliable
atmospheric parameters from high-resolution spectroscopic analysis. Our
approach was able to recover the parameters within 80 K for Teff, 0.05 dex for
[Fe/H] and 0.15 dex for log g, values that are lower or equal to the typical
external uncertainties found between different high-resolution analyzes. An
additional test was performed with a subsample of 138 stars from the ELODIE
stellar library and the literature atmospheric parameters were recovered within
125 K for Teff, 0.10 dex for [Fe/H] and 0.29 dex for log g. These results show
that the spectral indices are a competitive tool to characterize stars with the
intermediate resolution spectra.Comment: Accepted for publication in AJ. Abstract edited to comply with arXiv
standards regarding the number of character
Identification of a new murine tumor necrosis factor receptor locus that contains two novel murine receptors for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL).
Tumor necrosis factor (TNF) ligand and receptor superfamily members play critical roles in diverse developmental and pathological settings. In search for novel TNF superfamily members, we identified a murine chromosomal locus that contains three new TNF receptor-related genes. Sequence alignments suggest that the ligand binding regions of these murine TNF receptor homologues, mTNFRH1, -2 and -3, are most homologous to those of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptors. By using a number of in vitro ligand-receptor binding assays, we demonstrate that mTNFRH1 and -2, but not mTNFRH3, bind murine TRAIL, suggesting that they are indeed TRAIL receptors. This notion is further supported by our demonstration that both mTNFRH1:Fc and mTNFRH2:Fc fusion proteins inhibited mTRAIL-induced apoptosis of Jurkat cells. Unlike the only other known murine TRAIL receptor mTRAILR2, however, neither mTNFRH2 nor mTNFRH3 has a cytoplasmic region containing the well characterized death domain motif. Coupled with our observation that overexpression of mTNFRH1 and -2 in 293T cells neither induces apoptosis nor triggers NFkappaB activation, we propose that the mTnfrh1 and mTnfrh2 genes encode the first described murine decoy receptors for TRAIL, and we renamed them mDcTrailr1 and -r2, respectively. Interestingly, the overall sequence structures of mDcTRAILR1 and -R2 are quite distinct from those of the known human decoy TRAIL receptors, suggesting that the presence of TRAIL decoy receptors represents a more recent evolutionary event
Probing the Environment with Galaxy Dynamics
I present various projects to study the halo dynamics of elliptical galaxies.
This allows one to study the outer mass and orbital distributions of
ellipticals in different environments, and the inner distributions of groups
and clusters themselves.Comment: 5 pages, 2 figs, to appear in Proc. ESO Workshop, Groups of Galaxies
in the Nearby Universe (5-9 Dec 2005), eds. I. Saviane, V. Ivanov & J.
Borissova (Springer-Verlag
Arcabouço cronoestratigráfico da Formação Mafra (intervalo médio) na região de Rio Negro/PR - Mafra/SC, borda leste da bacia do Paraná
Investigating the topology of interacting networks - Theory and application to coupled climate subnetworks
Network theory provides various tools for investigating the structural or
functional topology of many complex systems found in nature, technology and
society. Nevertheless, it has recently been realised that a considerable number
of systems of interest should be treated, more appropriately, as interacting
networks or networks of networks. Here we introduce a novel graph-theoretical
framework for studying the interaction structure between subnetworks embedded
within a complex network of networks. This framework allows us to quantify the
structural role of single vertices or whole subnetworks with respect to the
interaction of a pair of subnetworks on local, mesoscopic and global
topological scales.
Climate networks have recently been shown to be a powerful tool for the
analysis of climatological data. Applying the general framework for studying
interacting networks, we introduce coupled climate subnetworks to represent and
investigate the topology of statistical relationships between the fields of
distinct climatological variables. Using coupled climate subnetworks to
investigate the terrestrial atmosphere's three-dimensional geopotential height
field uncovers known as well as interesting novel features of the atmosphere's
vertical stratification and general circulation. Specifically, the new measure
"cross-betweenness" identifies regions which are particularly important for
mediating vertical wind field interactions. The promising results obtained by
following the coupled climate subnetwork approach present a first step towards
an improved understanding of the Earth system and its complex interacting
components from a network perspective
Longitudinal Patterns of Change in Systolic Blood Pressure and Incidence of Cardiovascular DiseaseNovelty and Significance: The Atherosclerosis Risk in Communities Study
Elevated blood pressure in mid-life contributes significantly to the risk of cardiovascular disease. However, patterns of blood pressure increase may differ among individuals and may result in differential risk. Our goal was to examine the contribution of longitudinal patterns of blood pressure change to incidence of heart failure, coronary heart disease, stroke and cardiovascular disease mortality
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