Anchoring climate change communications

Verbal probability expressions (VPEs) are frequently used to communicate risk and uncertainty. The Intergovernmental Panel on Climate Change attempts to standardise the use and interpretation of these expressions through a translation scale of numerical ranges to VPEs. A common issue in interpreting VPEs is the tendency for individuals to interpret VPEs around the mid-point of the scale (i.e., around 50%). Previous research has shown that compliance with the IPCC’s standards can be improved if the numerical translation is presented simultaneously with the VPE, reducing the regressiveness of interpretations. We show that an explicit statement of the lower or upper bound implied by the expression (e.g., 0-33%; 66-100%) leads to better differentiated estimates of the probability implied by ‘likely’ and ‘unlikely’ than when the bound is not explicitly identified (e.g., less than 33%; greater than 66%)

Reconciling Deep Calibration and Demographic History: Bayesian Inference of Post Glacial Colonization Patterns in Carcinus aestuarii (Nardo, 1847) and C. maenas (Linnaeus, 1758)

A precise inference of past demographic histories including dating of demographic events using Bayesian methods can only be achieved with the use of appropriate molecular rates and evolutionary models. Using a set of 596 mitochondrial cytochrome c oxidase I (COI) sequences of two sister species of European green crabs of the genus Carcinus (C. maenas and C. aestuarii), our study shows how chronologies of past evolutionary events change significantly with the application of revised molecular rates that incorporate biogeographic events for calibration and appropriate demographic priors. A clear signal of demographic expansion was found for both species, dated between 10,000 and 20,000 years ago, which places the expansions events in a time frame following the Last Glacial Maximum (LGM). In the case of C. aestuarii, a population expansion was only inferred for the Adriatic-Ionian, suggestive of a colonization event following the flooding of the Adriatic Sea (18,000 years ago). For C. maenas, the demographic expansion inferred for the continental populations of West and North Europe might result from a northward recolonization from a southern refugium when the ice sheet retreated after the LGM. Collectively, our results highlight the importance of using adequate calibrations and demographic priors in order to avoid considerable overestimates of evolutionary time scales

Environmental enrichment and neurotransmitter receptors.

The extent of high affinity, specific binding of several pharmacological agents to brain membrane fractions derived from rats reared in an environmentally enriched or impoverished environment has been assayed. The binding of all labeled agents studied was not significantly altered in cerebellar, subcortical, or cortical membranes by the rearing condition. In the case of cortical membranes, binding of 3H-benzodiazepine, 3H-dihydroalprenolol, 3H-dihydroergocryptine, 3H-spiroperidol, 3H-quinuclidinyl benzilate (QNB), and 3H-muscimol was not detectably altered by exposure to these two housing conditions. These data suggest that the observed small but statistically significant increase in density of cortical synapses in rats kept in the "enriched" condition may not necessarily reflect a great increase in a specific class of synapse. © 1982 Academic Press, Inc

Molecular cloning and expression of a chloride ion channel of cell nuclei

Ion channels are known to be present on the plasma membrane of virtually all cells and have been found on the membranes of various intracellular organelles. However, until recently they were believed not to occur at the nuclear membrane. In this study we describe the molecular cloning and characterization of a nuclear ion channel protein, designated nuclear chloride channel-27 (NCC27), from the human myelomonocytic cell line, U937. NCC27 is a novel chloride ion channel protein that was found to localize principally to the cell nucleus. Its only known homologue is a bovine chloride ion channel protein (p64) believed to localize to internal organelles. NCC27 therefore represents the first human member of a new class of organella chloride ion channel proteins

Immunohistochemical Detection of Phospho-Akt, Phospho-BAD, HER2 and Oestrogen Receptors alpha and beta in Malaysian Breast Cancer Patients

Activation of Akt signaling pathway has been documented in various human malignancies, including breast carcinoma. The objective of this study is to determine the incidence of Akt phosphorylation in breast tumours and its relationship with expression of ER-α, ER-β, HER2, Ki-67 and phosphorylated Bcl-2 associated death domain (p-BAD). Immunohistochemical staining was performed to detect these molecules on 43 paraffin-embedded breast tumour tissues with commercially available antibodies. Eighteen (41.9%), 3 (7.0%), 23 (53.5%), 35 (81.4%), 21 (48.8%), 29 (67.4%), and 34 (81.0%) of breast tumours were positive for nuclear ER-α, nuclear ER-β, membranous HER2, cytonuclear p-Akt (Thr308), p-Akt (Ser473), p-BAD and Ki-67, respectively. ER-α expression was inversely correlated with HER2 and Ki-67 (P=0.041 and P=0.040, respectively). The p-Akt (Ser473) was correlated with increased level of p-BAD (Ser136) (P=0.012). No relationship of Akt phosphorylation with HER2, ER-α or ER-β was found. The p-Akt (Ser473) immunoreactivity was significantly higher in stage IV than in stage I or II (P=0.036 or P=0.009). The higher Ki-67 and lower ER-α expression showed an association with patient age of <50 years (P=0.004) and with positive nodal status (P=0.033), respectively. Our data suggest that the Akt phosphorylation and inactivation of its downstream target, BAD may play a role in survival of breast cancer cell. This study does not support the simple model of linear HER2/ PI3K/Akt pathway in breast cancer