Fluoxetine effects on periodontogenesis: histomorphometrical and immunohistochemical analyses in rats

Reports have indicated that serotonin plays an important role in cell migration and differentiation during the organogenesis of several tissues, including the oral types. Administration of selective serotonin reuptake inhibitor (SSRI) drugs during pregnancy could affect the delivery of serotonin to embryonic tissues altering its development. Objective This study aimed to assess the effects of fluoxetine, a selective serotonin reuptake inhibitor, on the formation of the periodontal ligament during pregnancy and lactation in rat pups. Material and Methods Twelve pregnant rats of Wistar lineage were divided into four study groups. In the control group, 0.9% sodium chloride solution was administered orally, throughout the entire period of the 21 days of pregnancy (CG group) and in the CGL group, it was administrated during pregnancy and lactation (from day 1 of pregnancy to the 21st day after birth). Fluoxetine was administered orally at the dose of 20 mg/kg in a group treated during pregnancy only (FG group), and during pregnancy and lactation (FGL group). Histometrical, histochemical and immunohistochemical analysis of the maxillary first molar periodontium region of the 24 rat pups was made under light microscopy, and periodontal ligament collagen was qualitatively evaluated under a polarizing light microscope. Results The quantity of fibroblasts (p=0.006), osteoblasts (p=0.027) and cementoblasts (p=0.001) was reduced in pups from the rats that received fluoxetine during pregnancy and lactation. No alterations were seen in the collagen fibers. Conclusion These findings suggest that periodontal tissue may be sensitive to fluoxetine, and its interference in reducing periodontal cells depends on exposure time during lactation

Histological and histometric aspects of radicular dentinogenesis in upper first molars of fluoxetine treated rats / Aspectos histológicos e histométricos da dentinogênese radicular em primeiros molares superiores dos ratos tratados com fluoxetina

Introduction: Serotonin is a neurotransmitter implicated on the control of several bodies roles including the regulation of circadian rhythm, of sleepiness, body temperature, motor and cognitive activities and of growth and development of tissues during embryogenesis. Objective: The aim of the present study is to assay the morphological aspects of the root dentin of upper first molars from pups of rats that were treated with fluoxetine in pregnancy. Methodology: For such, we conducted a random experimental study by using used 12 Wistar pregnant rats divided into three groups: C, FX and FL group. From the first to the 21 day of pregnancy, the rats received saline solution or fluoxetine hydrochloride solution, subcutaneously, according to the group to which they belonged. Subsequently, the offspring of animals was divided into subsets according to the age of tooth germ development to be studied: C20 and C45 (control group of 20 and 45 days of age); FL20 and FL45 (treated group with fluoxetine 10 mg/kg body weight of 20 and 45 days of life) and FX20 and FX45 (treated group with fluoxetine 20 mg/kg body weight of 20 and 45 days old). The thickness of root dentin, predentin thickness and length of odontoblasts were analyzed at the offspring samples. The data were tabulated and statistical values were performed with a significance level p 0,05. Results/Conclusion: Studies have shown significant changes in dentinogenesis root of the groups treated with fluoxetine at doses of 10 and 20 mg / kg body weight during pregnancy

Fluoxetine effects on periodontogenesis: histomorphometrical and immunohistochemical analyses in rats

Abstract Reports have indicated that serotonin plays an important role in cell migration and differentiation during the organogenesis of several tissues, including the oral types. Administration of selective serotonin reuptake inhibitor (SSRI) drugs during pregnancy could affect the delivery of serotonin to embryonic tissues altering its development. Objective This study aimed to assess the effects of fluoxetine, a selective serotonin reuptake inhibitor, on the formation of the periodontal ligament during pregnancy and lactation in rat pups. Material and Methods Twelve pregnant rats of Wistar lineage were divided into four study groups. In the control group, 0.9% sodium chloride solution was administered orally, throughout the entire period of the 21 days of pregnancy (CG group) and in the CGL group, it was administrated during pregnancy and lactation (from day 1 of pregnancy to the 21st day after birth). Fluoxetine was administered orally at the dose of 20 mg/kg in a group treated during pregnancy only (FG group), and during pregnancy and lactation (FGL group). Histometrical, histochemical and immunohistochemical analysis of the maxillary first molar periodontium region of the 24 rat pups was made under light microscopy, and periodontal ligament collagen was qualitatively evaluated under a polarizing light microscope. Results The quantity of fibroblasts (p=0.006), osteoblasts (p=0.027) and cementoblasts (p=0.001) was reduced in pups from the rats that received fluoxetine during pregnancy and lactation. No alterations were seen in the collagen fibers. Conclusion These findings suggest that periodontal tissue may be sensitive to fluoxetine, and its interference in reducing periodontal cells depends on exposure time during lactation