Procedimentos para o monitoramento populacional de Diaphorina Citri , vetor do Huanglongbing (HLB) dos citros.

Após o registro do agente etiológico do HLB, a bactéria Candidatus liberibacter spp. no Brasil em 2004, o inseto vetor Diaphorina citri (Hemiptera: Psyllidae) antes considerado uma praga secundária dos citros, ganhou o status de inseto-praga de grande importância

Monitoramento populacional de Diaphorina citri Kuwayama, 1908 (Hemiptera: Liviidae) em cinco municípios do Recôncavo da Bahia.

O psilídeo Diaphorina citri é um importante vetor da bactéria Candidatus liberibacter spp., causadora do HLB (Huaglongbing), doença mais devastadora dos citros, assumindo, assim, o status de principal praga nessa cultura. O agente etiológico do HLB foi registrado no Brasil em 2004, primeiramente em São Paulo e, posteriormente, em Minas Gerais e Paraná

Understanding the dynamic of tropical agriculture for remote sensing applications: a case study of Southeastern Brazil.

Abstract: The agricultural activity can greatly benefit from remote sensing technology (RS). Optical passive RS has been vastly explored for agricultural mapping and monitoring, in despite of cloud cover issue. This is observed even in the tropics, where frequency of clouds is very high. However, more studies are needed to better understand the high dynamism of tropical agriculture and its impact on the use of passive RS. In tropical countries, such as in Brazil, the use of current agricultural technologies, associated with favourable climate, allow the planting period to be wide and to have plants of varying phenological cycles. In this context, the main objective of the current study is to better understand the dynamics of a selected area in Southeast of São Paulo state, and its impact on the use of orbital passive RS. For that purpose, data (from field and satellite) from 55 agricultural fields, including annual, semi-perennial and perennial crops and silviculture, were acquired between July 2014 and December 2016. Field campaigns were conducted in a monthly base to gather information about the condition of the crops along their development (data available in a website). Field data corresponding to the 2014-2015 crop year were associated with a time series of Landsat-8/OLI RGB false-colour compositions images and MODIS/Terra NDVI profiles. The type of information that can be extracted (such as specie identification, crop management practices adopted, date of harvest, type o production system used etc) by combining passive remote sensing data with field data is discussed in the paper

Geometric compensation applied to image analysis of cell populations with morphological variability: A new role for a classical concept

Immunofluorescence is the gold standard technique to determine the level and spatial distribution of fluorescent-tagged molecules. However, quantitative analysis of fluorescence microscopy images faces crucial challenges such as morphologic variability within cells. In this work, we developed an analytical strategy to deal with cell shape and size variability that is based on an elastic geometric alignment algorithm. Firstly, synthetic images mimicking cell populations with morphological variability were used to test and optimize the algorithm, under controlled conditions. We have computed expression profiles specifically assessing cell-cell interactions (IN profiles) and profiles focusing on the distribution of a marker throughout the intracellular space of single cells (RD profiles). To experimentally validate our analytical pipeline, we have used real images of cell cultures stained for E-cadherin, tubulin and a mitochondria dye, selected as prototypes of membrane, cytoplasmic and organelle-specific markers. The results demonstrated that our algorithm is able to generate a detailed quantitative report and a faithful representation of a large panel of molecules, distributed in distinct cellular compartments, independently of cell's morphological features. This is a simple end-user method that can be widely explored in research and diagnostic labs to unravel protein regulation mechanisms or identify protein expression patterns associated with disease.This work was supported by FEDER funds through the Operational Programme for Competitiveness Factors (COMPETE) and National Funds through the Portuguese Foundation for Science and Technology (FCT), under the projects PTDC/BIM-ONC/0171/2012, PTDC/BIM-ONC/0281/2014, PTDC/BBB-IMG/0283/2014; Post-Doctoral grants SFRH/BPD/87705/2012-JF and SFRH/BPD/104208/2014-BS; and Doctoral grant SFRH/ BD/108009/2015-SM. We acknowledge the Programa IFCT (FCT Investigator) for funding JP research. We also thank to the American Association of Patients with Hereditary Gastric Cancer “No Stomach for Cancer” for funding the projects “Today’s present, tomorrow’s future on the study of germline E-cadherin missense mutations” and “Today’s Present, Tomorrow’s Future on the Study of Germline E-Cadherin Missense Mutations: A Step Forward on Providing Informed Genetic Counseling to Everyone”

Flutuação populacional de Diaphorina citri (Hemiptera: Psyllidae), vetor do HLB (huanglongbing) no Recôncavo da Bahia.

O psilídeo Diaphorina citri é, atualmente, o inseto vetor da doença mais devastadora dos citros. Ganhou esse status por ser o transmissor da bactéria Candidatus Liberibacter spp., causadora, do HLB. De origem asiática, o HLB foi detectado no Brasil em 2004 e encontra-se disseminada no estado de São Paulo, e em vários municípios de Minas Gerais e Paraná

Anti-oxLDL autoantibodies and their correlation with lipid profile and nutritional status in adolescents

OBJECTIVE: To investigate whether levels of autoantibodies to oxidized LDL (anti-oxLDL) in the plasma of adolescents correlates with their anthropometric measurements and lipid profiles. METHODS: The study enrolled 150 adolescents aged between 10 and 15 years, recruited from the obesity clinic at Universidade Federal de São Paulo (UNIFESP) (SP) and from public schools in Piracicaba, SP, Brazil. Anthropometric measurements such as body mass index and waist and arm circumferences were used to classify the adolescents as having healthy weight, overweight or obesity. Colorimetric enzymatic methods were used for biochemical lipid profile analysis and ELISA was used to determine anti-oxLDL autoantibody levels. RESULTS: Analysis of anthropometric variables indicated that the obese group's profile was abnormal compared to the healthy weight and overweight groups (p < 0.01), indicating cardiovascular risk. Analysis of the lipid profiles demonstrated statistically significant differences in concentrations of total cholesterol (p = 0.011), HDL-cholesterol (p = 0.001) and LDL-cholesterol (p < 0.042) between the healthy weight group and the obese group. Analysis of plasma anti-oxLDL autoantibodies demonstrated that the overweight (p = 0.012) and obese groups (p < 0.001) had higher values than the healthy weight group. There were also correlations between anti-oxLDL autoantibody levels and anthropometric variables. CONCLUSIONS: In adolescents the presence of anti-oxLDL autoantibodies and metabolic changes to the lipid profile vary in proportion with anthropometric parameters, which makes anti-oxLDL concentration a potential biochemical indicator of risk of metabolic syndrome.OBJETIVO: Avaliar se o conteúdo de auto-anticorpos anti-LDL oxidada (anti-LDLox) no plasma de adolescentes correlaciona-se com suas medidas antropométricas e com o perfil lipídico. MÉTODOS: O estudo incluiu 150 adolescentes com idade entre 10 e 15 anos, recrutados do ambulatório de obesidade da Universidade Federal de São Paulo (UNIFESP) (SP) e de escolas públicas de Piracicaba (SP). Foram avaliadas medidas antropométricas, como índice de massa corporal, circunferência de cintura e do braço, classificando os adolescentes em eutrófico, sobrepeso e obeso. Para as análises bioquímicas, foi realizado o perfil lipídico através de métodos enzimáticos colorimétricos, e para detecção do conteúdo de auto-anticorpos anti-LDLox, utilizou-se o método de ELISA. RESULTADOS: Segundo análises das variáveis antropométricas, o grupo obeso apresentou perfil alterado em relação aos grupos eutrófico e sobrepeso (p < 0,01), indicando risco cardiovascular. Quando o perfil lipídico foi avaliado, observaram-se diferenças estatisticamente significativas para as concentrações de colesterol total (p = 0,011), HDL-colesterol (p = 0,001) e LDL-colesterol (p < 0,042) nos grupos eutrófico e obeso. Para as análises de auto-anticorpos anti-LDLox plasmática, os grupos sobrepeso (p = 0,012) e obeso (p < 0,001) apresentaram valores superiores ao grupo eutrófico. Também houve correlações entre os auto-anticorpos anti-LDLox e variáveis antropométricas. CONCLUSÃO: A presença de auto-anticorpos anti-LDLox em adolescentes e as alterações metabólicas no perfil lipídico variaram de modo proporcional com parâmetros antropométricos, o que torna o conteúdo de anti-LDLox um potencial indicador bioquímico de risco para síndrome metabólica.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade de São PauloUniversidade Federal de São Paulo (UNIFESP) Departamento de PediatriaUniversidade Federal de São Paulo (UNIFESP) Centro de Atendimento e Apoio ao AdolescenteUniversidade Federal de São Paulo (UNIFESP)Universidade Federal de São Paulo (UNIFESP) CAAAUSP Faculdade de Saúde PúblicaUNIFESP, Depto. de PediatriaUNIFESP, Centro de Atendimento e Apoio ao AdolescenteUNIFESP, CAAASciEL

Surface Modification of Bioresorbable Phosphate Glasses for Controlled Protein Adsorption

The traditional silicate bioactive glasses exhibit poor thermal processability, which inhibits fiber drawing or sintering into scaffolds. The composition of the silicate glasses has been modified to enable hot processing. However, the hot forming ability is generally at the expense of bioactivity. Metaphosphate glasses, on the other hand, possess excellent thermal processability, congruent dissolution, and a tailorable degradation rate. However, due to the layer-by-layer dissolution mechanism, cells do not attach to the material surface. Furthermore, the congruent dissolution leads to a low density of OH groups forming on the glass surface, limiting the adsorption of proteins. It is well regarded that the initial step of protein adsorption is critical as the cells interact with this protein layer, rather than the biomaterial itself. In this paper, we explore the possibility of improving protein adsorption on the surface of phosphate glasses through a variety of surface treatments, such as washing the glass surface in acidic (pH 5), neutral, and basic (pH 9) buffer solutions followed or not by a treatment with (3-aminopropyl)triethoxysilane (APTS). The impact of these surface treatments on the surface chemistry (contact angle, ζ-potential) and glass structure (FTIR) was assessed. In this manuscript, we demonstrate that understanding of the material surface chemistry enables to selectively improve the adsorption of albumin and fibronectin (used as model proteins). Furthermore, in this study, well-known silicate bioactive glasses (i.e., S53P4 and 13-93) were used as controls. While surface treatments clearly improved proteins adsorption on the surface of both silicate and phosphate glasses, it is of interest to note that protein adsorption on phosphate glasses was drastically improved to reach similar protein grafting ability to the silicate bioactive glasses. Overall, this study demonstrates that the limited cell/phosphate glass biological response can easily be overcome through deep understanding and control of the glass surface chemistry