Cytogenetic Study Of Anopheles Albitarsis (diptera: Culicidae) By C-banding And In Situ Hybridization

The C-banding pattern and the size and location of the nucleolar organizer regions (NORs) are described for the first time in Brazilian populations of Anopheles (Nyssorhynchus) albitarsis sensu lato. C-banding revealed variation in the size of the centromeric heterochromatic blocks in autosomal chromosomes and in the acrocentric (X) and puntiform (Y) sex chromosomes. Fluorescence in situ hybridization showed that the NORs were located in the pericentromeric region of the sex (XX/XY) chromosomes and that this coincided with the number and location of centromeric constitutive heterochromatin blocks previously revealed by C-banding. The NORs varied in size among the homologues of the three populations. These findings of the populations studied support the hypothesis that the stability of NORs in the A. albitarsis complex is characterized by the presence of clustered and conserved sites in a unique pair of chromosomes.14320066267Baimai, V., Heterochromatin accumulation and karyotypic evolution in some dipteran insects (1998) Zool. Stud, 37, pp. 75-88Baimai, V., Harrison, B.A., Heterochromatin variation in the sex chromosomes in Thailand populations of Anopheles dirus A (Diptera: Culicidae) (1984) Can. J. Genet. Cytol, 26, pp. 633-636Baimai, V., Traipakvasin, A., Intraspecific variation in sex heterochromatin of species B of the Anopheles dirus complex in Thailand (1987) Genome, 29, pp. 401-404Baimai, V., Kijchalao, U., Rattanarithikul, R., Metaphase karyotypes of Anopheles of Thailand and southeast Asia: II. maculatus group, Neocellia series, subgenus Cellia (1993) Mosq. Syst, 25, pp. 116-123Baimai, V., Treesucon, A., Kijchalao, U., Heterochromatin variation in chromosome X in a natural population of Anopheles willmori (Diptera, Culicidae) of Thailand (1996) Genetica, 97, pp. 235-239Conn, J.E., Wilkerson, R.C., Segura, M.N.O., Emergence of a new Neotropical malaria vector facilitated by human migration and changes in land use (2002) Am. J. Trop. Med. Hyg, 66, pp. 18-22Faran, M.E., Linthicum, K.J., A handbook of the Amazonian species of Anopheles (Nyssorhynchus) (Diptera: Culicidae) (1981) Mosq. Syst, 13, pp. 1-91Forattini, O.P., (1962) Entomologia Médica, , Faculdade de Higiene e Saúde Pública da USP, Editora da USP(2000) FUNASA - Fundação Nacional de Saúde, , http://www.funasa.gov.br/epi/malaria/pdfs/plano€taduais.pdfGale, K.R., Crampton, J.M., The ribosomal genes of the mosquito, Aedes aegypti (1989) Eur. J. Biochem, 185, pp. 311-317Gatti, M., Bonaccorsi, S., Pimpinelli, S. et al. 1982. Polymorphism of sex chromosome heterochromatin in the Anopheles gambiae complex. - In: Steiner, W. W. M., Tabachnick, W. J., Rai, K. S. et al. (eds), Recent developments in the genetics of insect disease vectors. Stipes Publ. Co, Champaign, IL, p. 32-48Gorham, J.R., Stojanovich, C.J., Scott, H.G., (1967) Chave ilustrada para los mosquitos anofelinos de Sudamerica Oriental, , Dept of Health, Education and Welfare, Public Health ServiceImai, H.T., Taylor, R.W., Crosdland, W.J., Modes of spontaneous chromosomal mutation and karyotype evolution in ants with reference to the minimum interaction hypothesis (1988) Jap. J. Genet, 63, pp. 159-185Jamilena, M., Ruiz Rejón, C., Ruiz Rejón, M., Variation in the heterochromatin and nucleolar organizing regions of Allium subvillosum L. (Liliaceae) (1990) Genome, 33, pp. 779-784Kreutzer, R.D., Kitzmiller, J.B., Rabbani, M.G., Cytogenetically distinguishable sympatric and allopatric populations of the mosquito Anopheles albitarsis (1976) Acta Amazonica, 6, pp. 473-481Kumar, A., Rai, K.S., Chromosomal localization and copy number of 18S + 28S ribosomal RNA genes in evolutionary diverse mosquitoes (Diptera: Culicidae) (1990) Hereditas, 113, pp. 277-289Marchi, A., Mezzanotte, R., Inter- and intraspecific heterochromatin variation detected by restriction endonuclease digestion in two sibling species of the Anopheles maculipennis complex (1990) Heredity, 65, pp. 135-142Marchi, A., Pili, E., Ribosomal RNA genes in mosquitoes: Localization by fluorescence in situ hybridization (FISH) (1994) Heredity, 72, pp. 599-605Munstermann, L.E., Conn, J.E., Systematics of mosquito disease vectors (Diptera, Culicidae): Impact of molecular biology and cladistic analysis (1997) Annu. Rev. Entomol, 42, pp. 351-369Póvoa, M., Wirtz, R., Lacerda, R., Malaria vectors in the municipality of Serra do Navio, State of Amapa, Amazon Region, Brazil (2001) Mem Inst Oswaldo Cruz, 96, pp. 179-184Rafael, M.S., Tadei, W.P., Metaphase karyotypes of Anopheles (Nyssorhynchus) darlingi Root and A. (N.) nuneztovari Gabaldón (DipteraCulicidae) (1998) Genet. Mol. Biol, 21, pp. 351-354Rafael, M.S., Tadei, W.P., Heterochromatin variation in chromosomes of Anopheles (Nyssorhynchus) darlingi Root and A. (N.) nuneztovari Gabaldón (Diptera: Culicidae). b (2000) Genet. Mol. Biol, 23, pp. 67-70Rafael, M.S., Tadei, W.P., Recco-Pimentel, S.M., Location of ribosomal genes in the chromosomes of Anopheles darlingi and Anopheles nuneztovari (Diptera, Culicidae) from the Brazilian Amazon (2003) Mem. Inst. Oswaldo Cruz, 98, pp. 629-635Rafael, M.S., Santos-Junior, I.P., Tadei, W.P., Karyotype of Brazilian Anopheles albitarsis sensu lato (Diptera: Culicidae) (2005) Genet. Mol. Res, 4, pp. 684-690Ramírez, C.C.L., (1989) Estudo cromossômico em uma população de Anopheles (Kerteszia) cruzii Dyar & Knab, 1909, , Tese de Mestrado, Inst de Biociências, USP, São PauloRamírez, C.C.L., Dessen, E.M.B., Cytogenetic analysis of natural population of Anopheles cruzii (1994) Rev. Bras. Genet, 17, pp. 41-46Rao, P.N., Rai, K.S., Comparative karyotypes and chromosomal evolution in some genera of Nematocerous (Diptera, Nematocera) families (1987) Ann. Entomol. Soc. Am, 80, pp. 321-332Silva-Vasconcelos, A., Kato, M.Y., Mourão, E.M., Biting indices, host-seeking activity and natural infection rates of anopheline species in Boa Vista, Roraima, Brazil from 1996 to 1998 (2002) Mem. Inst. Oswaldo Cruz, 97, pp. 151-161Sumner, A.T., A simple technique for demonstrating centromeric heterochromatin (1972) Exp. Cell Res, 75, pp. 304-305Tadei, W.P., Dutary-Thatcher, B., Malaria vectors in the Brazilian Amazon: Anopheles of the subgenus Nyssorhynchus (2000) Rev. Inst. Med. Trop. S. Paulo, 42, pp. 87-94Vasantha, K., Subbarao, S.K., Adak, T., Karyotypic variations in Anopheles culifacies complex (1982) Ind. J. Malariol, 19, pp. 27-32Viegas-Pequignot, E., In situ hybridization to chromosomes with biotinylated probes (1992) In situ hybridization: Practical approach, pp. 137-158. , Willman, D, ed, Oxford Univ. Press: IRL Press, p