Human intestinal anion exchanger isoforms: expression, distribution, and membrane localization

AbstractA family of anion exchangers (AEs) including AE1, AE2 and AE3 has been described. AE3 gene has been shown to encode two alternatively spliced isoforms termed as bAE3 (brain subtype) and cAE3 (cardiac subtype). The identity of the AE(s) involved in the human intestinal NaCl absorption is not fully understood. Current studies were undertaken to identify the AE isoforms expressed in the human intestine, to define their regional and vertical axis (crypt vs. surface cells) distribution, and to elucidate their membrane localization in the epithelial cells along the entire length of the human intestine. Our studies utilizing reverse transcription (RT)-PCR with total RNA extracted from pinch biopsies from various regions of the human intestine demonstrate that AE2 and bAE3 but not AE1 or cAE3 were expressed in all the regions of the human intestine. Utilizing in situ RT-PCR, we demonstrated that the message of AE2 was expressed throughout the vertical surface–crypt axis of the colon. Our Western blotting studies demonstrated that AE2 and bAE3 are localized to the basolateral but not the apical membranes of the intestinal epithelial cells from the human ileum and colon. In conclusion, our results demonstrated that in the human intestine, AE2 and bAE3, but not AE1 or cAE3, are expressed throughout the tract with the highest expression in the colon compared to the ileum and jejunum. Both the isoforms were found to be localized to the basolateral but not the apical membranes of the epithelial cells. We speculate that, in the human intestine, AE2 and bAE3 may be the ‘housekeeping’ isoforms, and the apical AE, the potential candidate for chloride absorption, remains to be identified

Iterative algorithms for total variation-like reconstructions in seismic tomography

A qualitative comparison of total variation like penalties (total variation, Huber variant of total variation, total generalized variation, ...) is made in the context of global seismic tomography. Both penalized and constrained formulations of seismic recovery problems are treated. A number of simple iterative recovery algorithms applicable to these problems are described. The convergence speed of these algorithms is compared numerically in this setting. For the constrained formulation a new algorithm is proposed and its convergence is proven.Comment: 28 pages, 8 figures. Corrected sign errors in formula (25

Quantum Criticality via Magnetic Branes

Holographic methods are used to investigate the low temperature limit, including quantum critical behavior, of strongly coupled 4-dimensional gauge theories in the presence of an external magnetic field, and finite charge density. In addition to the metric, the dual gravity theory contains a Maxwell field with Chern-Simons coupling. In the absence of charge, the magnetic field induces an RG flow to an infrared AdS$_3 \times {\bf R}^2$ geometry, which is dual to a 2-dimensional CFT representing strongly interacting fermions in the lowest Landau level. Two asymptotic Virasoro algebras and one chiral Kac-Moody algebra arise as {\sl emergent symmetries} in the IR. Including a nonzero charge density reveals a quantum critical point when the magnetic field reaches a critical value whose scale is set by the charge density. The critical theory is probed by the study of long-distance correlation functions of the boundary stress tensor and current. All quantities of major physical interest in this system, such as critical exponents and scaling functions, can be computed analytically. We also study an asymptotically AdS$_6$ system whose magnetic field induced quantum critical point is governed by a IR Lifshitz geometry, holographically dual to a D=2+1 field theory. The behavior of these holographic theories shares important similarities with that of real world quantum critical systems obtained by tuning a magnetic field, and may be relevant to materials such as Strontium Ruthenates.Comment: To appear in Lect. Notes Phys. "Strongly interacting matter in magnetic fields" (Springer), edited by D. Kharzeev, K. Landsteiner, A. Schmitt, H.-U. Ye

Boson-fermion unification, superstrings, and Bohmian mechanics

Bosonic and fermionic particle currents can be introduced in a more unified way, with the cost of introducing a preferred spacetime foliation. Such a unified treatment of bosons and fermions naturally emerges from an analogous superstring current, showing that the preferred spacetime foliation appears only at the level of effective field theory, not at the fundamental superstring level. The existence of the preferred spacetime foliation allows an objective definition of particles associated with quantum field theory in curved spacetime. Such an objective definition of particles makes the Bohmian interpretation of particle quantum mechanics more appealing. The superstring current allows a consistent Bohmian interpretation of superstrings themselves, including a Bohmian description of string creation and destruction in terms of string splitting. The Bohmian equations of motion and the corresponding probabilistic predictions are fully relativistic covariant and do not depend on the preferred foliation.Comment: 30 pages, 1 figure, revised, to appear in Found. Phy

A comment on multiple vacua, particle production and the time dependent AdS/CFT correspondence

We give an explicit formulation of the time dependent AdS/CFT correspondence when there are multiple vacua present in Lorentzian signature. By computing sample two point functions we show how different amplitudes are related by cosmological particle production. We illustrate our methods in two example spacetimes: (a) a ``bubble of nothing'' in AdS space, and (b) an asymptotically locally AdS spacetime with a bubble of nothing on the boundary. In both cases the alpha vacua of de Sitter space make an interesting appearance.Comment: 9 page

Hope, optimism, and other business assets: Why “psychological capital” is so valuable to your company

Entrevista com Fred Luthans,1 coauthor of Psychological Capital: Developing the Human Competitive EdgeInterview with Fred Luthans,1 coauthor of Psychological Capital: Developing the Human Competitive Edg

Miracles and complementarity in de Sitter space

In this paper we consider a scenario, consisting of a de Sitter phase followed by a phase described by a scale factor $a(t)\sim t^{q}$, where $1/3<q<1$, which can be viewed as an inflationary toy model. It is argued that this scenario naively could lead to an information paradox. We propose that the phenomenon of Poincar\'{e} recurrences plays a crucial role in the resolution of the paradox. We also comment on the relevance of these results to inflation and the CMBR.Comment: 13 page

Astrophysical structures from primordial quantum black holes

The characteristic sizes of astrophysical structures, up to the whole observed Universe, can be recovered, in principle, assuming that gravity is the overall interaction assembling systems starting from microscopic scales, whose order of magnitude is ruled by the Planck length and the related Compton wavelength. This result agrees with the absence of screening mechanisms for the gravitational interaction and could be connected to the presence of Yukawa corrections in the Newtonian potential which introduce typical interaction lengths. This result directly comes out from quantization of primordial black holes and then characteristic interaction lengths directly emerge from quantum field theory.Comment: 11 page

Cosmological distance indicators

We review three distance measurement techniques beyond the local universe: (1) gravitational lens time delays, (2) baryon acoustic oscillation (BAO), and (3) HI intensity mapping. We describe the principles and theory behind each method, the ingredients needed for measuring such distances, the current observational results, and future prospects. Time delays from strongly lensed quasars currently provide constraints on $H_0$ with < 4% uncertainty, and with 1% within reach from ongoing surveys and efforts. Recent exciting discoveries of strongly lensed supernovae hold great promise for time-delay cosmography. BAO features have been detected in redshift surveys up to z <~ 0.8 with galaxies and z ~ 2 with Ly-$\alpha$ forest, providing precise distance measurements and $H_0$ with < 2% uncertainty in flat $\Lambda$CDM. Future BAO surveys will probe the distance scale with percent-level precision. HI intensity mapping has great potential to map BAO distances at z ~ 0.8 and beyond with precisions of a few percent. The next years ahead will be exciting as various cosmological probes reach 1% uncertainty in determining $H_0$, to assess the current tension in $H_0$ measurements that could indicate new physics.Comment: Review article accepted for publication in Space Science Reviews (Springer), 45 pages, 10 figures. Chapter of a special collection resulting from the May 2016 ISSI-BJ workshop on Astronomical Distance Determination in the Space Ag

Organic nitrate aerosol formation via NO³ + biogenic volatile organic compounds in the southeastern United States

Gas- and aerosol-phase measurements of oxidants, biogenic volatile organic compounds (BVOCs) and organic nitrates made during the Southern Oxidant and Aerosol Study (SOAS campaign, Summer 2013) in central Alabama show that a nitrate radical (NO₃) reaction with monoterpenes leads to significant secondary aerosol formation. Cumulative losses of NO₃ to terpenes are correlated with increase in gasand aerosol-organic nitrate concentrations made during the campaign. Correlation of NO₃ radical consumption to organic nitrate aerosol formation as measured by aerosol mass spectrometry and thermal dissociation laser-induced fluorescence suggests a molar yield of aerosol-phase monoterpene nitrates of 23–44 %. Compounds observed via chemical ionization mass spectrometry (CIMS) are correlated to predicted nitrate loss to BVOCs and show C₁₀H₁₇NO₅, likely a hydroperoxy nitrate, is a major nitrate-oxidized terpene product being incorporated into aerosols. The comparable isoprene product C₅H₉NO₅ was observed to contribute less than 1% of the total organic nitrate in the aerosol phase and correlations show that it is principally a gas-phase product from nitrate oxidation of isoprene. Organic nitrates comprise between 30 and 45% of the NOy budget during SOAS. Inorganic nitrates were also monitored and showed that during incidents of increased coarse-mode mineral dust, HNO₃ uptake produced nitrate aerosol mass loading at a rate comparable to that of organic nitrate produced via NO₃ CBVOCs