Transcriptional activator gene based phylogenetic analysis of dolichos yellow mosaic virus infecting lablab bean (Dolichos lablabL.)

Lablab bean (Dolichos lablab L.) is one the important crop, cultivated as vegetable, pulses as it is rich in protein. It is affected from viral disease i.e. dolichos yellow mosaic virus. The causative agent is begomovirus belongs to geminivirus family. Begomovirus contain bipartite genome having two type of DNA-A and DNA-B. DNA- A helps in replication and DNA-B helps in movement. These DNA have six different type of gene coat protein gene, transcriptional activator gene, replica-tion associated gene, replication enhancer gene, pre coat protein involved in different function associated with it. The present investigation was carried out to investigate the transcriptional activa-tor gene based phylogenetic analysis of Dolichos yellow mosaic virus infecting D. lablab. This study is based on the transcriptional activator gene which is used in transactivation of genes, contains three conserved domains: a basic domain at N-Terminus, a central DNA binding domain and activator domain. The genome databases of dolichos yellow mosaic virus were taken from NCBI site total six genome was available and were used with Clustal W and CLC BIO were the bioinformatic tools for determining sequence homology among genome present in different geographical location. The absence of functional specificity suggests that all begomovirus contains a common element interacts with cellular proteins of other viruses reveals the phylogenetic analysis with the other species Dolichos in the different geographical location

A Lightweight and Efficient Scheme for e-Health Care System using Blockchain Technology

We are witnessed to the huge amount of health care information is processed via Internet and uploaded to the servers. Hence, the network content suffers from various challenges in handling this wide range of information such as privacy-preservation, message requests, and sharing of information with the entities. Protection of health care information with less communication overhead and avoid signaling congestion among entities is the biggest concern. We suggest a simple and effective blockchain-based system for ehealth care in order to address these problems. We attempt to show the collective trust between receiver/ users and key generation center (KGC). Due to the characteristics such as tamper-resistant of transactions and responsiveness, the blockchain technology maintains the data access policy and privacy of each receiver/ users. In addition, we provide the public transactions apparently with the help of miners. Also, the performance analysis shows that the protocol is free from all the security attacks and avoid the signaling congestion with less overhead

Participatory evaluation of advanced potato (Solanum tuberosum) clones for water stress tolerance

An attempt was made to introduce potato (Solanum tuberosum L.) in hot arid water scarce zone of Western Rajasthan, India. Eight CIP-bred potato clones were evaluated along with two controls, viz. Kufri Pukhraj and Kufri Surya on farmer’s field at Jodhpur, Rajasthan under normal and moderate water stress regime during three winter crop seasons (2012-15). Pooled analysis revealed that CIP clone 397006.18 (34.0 tonnes/ha) out yielded Kufri Pukhraj (26.8 tonnes/ha) and Kufri Surya (20.2 tonnes/ha) for marketable tuber yield. This clone maintained yield under normal and deficit irrigation and attained 26.9% higher marketable yield under normal irrigations, which further improved by 31.3% under deficit water stress regime over the best control Kufri Pukhraj. This clone recorded highest tuber dry matter content (22.7%), statistically superior to both control Kufri Pukhraj (17.9%) and Kufri Surya (21.4%). Clone 397006.18 had highest mean drought tolerance index value (1.16) and least total weight loss under normal irrigation (4.9%) and water stress conditions (8.6%) up to 60 days of storage. Preference yield analysis by potato growers showed that they liked this clone most and its overall acceptability was fairly better for all organoleptic traits. Results of field study and participatory varietal selection indicated that clone 397006.18 performed better for tuber productivity under deficit water management, exhibited drought tolerance traits and achieved overall acceptance by the farmers in Western Rajasthan

D-PENICILLAMINE INHIBITS TRANSACTIVATION OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 (HIV-1) LTR BY TRANSACTIVATOR PROTEIN

AbstractD-Penicillamine, an amino acid analogue of cysteine, has been shown to inhibit the transactivation of HIV-1 LTR by the transactivator protein, tat protein. The transactivation was studied in Jurkat cells co-transfected with plasmids containing HIV-LTR sequences fused to the bacterial chloramphenicol acetyltransferase (CAT) gene and HIV tat gene. The expression of CAT activity was a measure of transactivation of LTR by the tat protein. Incubation of transfected Jurkat cells with D-penicillamine led to inhibition of CAT activity. This inhibition was found to be concentration-dependent; more than 90% inhibition of chloramphenicol acetylation was seen in extracts prepared from cultures incubated with 40 μg/ml of D-penicillamine. Earlier experiments have shown that D-penicillamine at 40 μg/ml can completely inhibit HIV-1 (HTLV-III B) replication in H9 cells [(1986) Drug Res. 36, 184–186]. These results suggest that inhibition of transactivation may be the molecular mechanism involved in the inhibition of HIV-1 replication by D-penicillamine

Sustainable intensification of potato in rice based system for increased productivity and income of resource poor farmers in West Bengal, India

The aim of present study was conducted was to increase productivity of exiting cropping systems and farmers income by expanding potato in non-traditional potato growing regions by adopting an innovative technology Double Transplanting (DT) of Rice and planting early maturing potato between two rice crops. This cropping pattern will enhance productivity of system without sacrificing area or productivity of either of the two crops. Similar work has been done earlier (Robin et al. 2007 and Burdhan Roy et al. 2007). The field trials were carried out at three locations Siliguri Subdivision of Darjeeling district to and Rajgunj block of Jalpaiguri district to introduce potato as sand witch crop between two rice crops. Eighty five farmers planted 18.6 acres land under rice-potato-rice system in 2013-14. Cost-benefit of different cropping systems was analyzed. Results revealed that “Potato-DT Rice” produced higher income/ha compared to traditional potato-boro rice and kharif (monsoon) rice-boro rice. The DT rice-potato system generated 27% higher income over traditional potato-boro rice and 290% over mono boro rice system. The gender responsive meetings, trainings and demonstrations were conducted. The farming community was motivated to adopt this new profitable cropping system in the region