The isolation of differentially expressed cDNA clones from the filarial nematode <i>Brugia pahangi</i>

A cDNA library constructed from 3 day post-infective L3 of the filarial nematode Brugia pahangi was screened by differential hybridization with cDNA probes prepared from different life-cycle stages. Five cDNA clones hybridizing selectively to the mosquito-derived L3 probe were isolated and characterized. Northern blot analysis of 4 of the clones confirmed that each was most highly expressed in the mosquito-derived L3. The expression of each mRNA during parasite development in the mosquito vector was investigated using RT-PCR, and all were shown to be abundant in the immature L3. Four of the 5 cDNAs cloned coded for structural proteins: 2 cuticular collagens, and the muscle proteins tropomyosin and troponin. Further studies on troponin using an antiserum raised to the recombinant protein demonstrated that the protein, unlike the mRNA, was present in all life-cycle stages examined, while immunogold labelling demonstrated that it was localized to the muscle blocks

Contesting powerful knowledge: The primary geography curriculum as an articulation between academic and children’s (ethno-) geographies

publication-status: Publishedtypes: ArticleThe argument has been propounded that academic disciplines and school subjects provide a powerful, authoritative knowledge which is key to enabling children to better understand the world in which they live. Inherent in this perspective is that children’s experience, knowledge and understanding is poorly formed and of limited everyday use and value. Yet it is appreciated that children’s naïve knowledge can be a pedagogic starting point to initiate them into academic subjects. While appreciating the purpose and roles of academic subjects, this paper challenges these assumptions, arguing that children’s ethno-knowledges provide powerful learning bases of equivalent authority to subjects. Using the example of younger children’s everyday or ethno-geography, the case is that children bring to school powerful (geographical) knowledge of their own. This can and should be recognised and valued in dialogue with authoritative (geographical) subject knowledge, not as subservient to it. It is argued that this perspective goes beyond that of the child/subject co-construction of knowledge to inter-relate the developmental nature of children’s everyday (geographical) learning with (geography) sense-of-subject evolution. This case is set in the context of geography but is applicable to other school subjects, where children’s and subjects’ powerful knowledges can mutually engage with and enhance each other

A solution concentration dependent transition from self-stratification to lateral phase separation in spin-cast PS:d-PMMA thin films

Thin films with a rich variety of different nano-scale morphologies have been produced by spin casting solutions of various concentrations of PS:d-PMMA blends from toluene solutions. During the spin casting process specular reflectivity and off-specular scattering data were recorded and ex situ optical and atomic force microscopy, neutron reflectivity and ellipsometry have all been used to characterise the film morphologies. We show that it is possible to selectively control the film morphology by altering the solution concentration used. Low polymer concentration solutions favour the formation of flat in-plane phase-separated bi-layers, with a d-PMMA-rich layer underneath a PS-rich layer. At intermediate concentrations the films formed consist of an in-plane phase-separated bi-layer with an undulating interface and also have some secondary phase-separated pockets rich in d-PMMA in the PS-rich layer and vice versa. Using high concentration solutions results in laterally phase-separated regions with sharp interfaces. As with the intermediate concentrations, secondary phase separation was also observed, especially at the top surface

Regina Caeli

The Jesuits were expelled from the Spanish colonies in South America in 1767, leaving behind a remarkable musical legacy that was buried for over two hundred years. But the music did not disappear completely. Thanks to the Chiquitos people of Bolivia, the music was played and preserved throughout the 18th and 19th centuries. In 1985, Swiss architect Hans Roth discovered 9,000 of these musical manuscripts and in 1990 UNESCO declared the churches of the Chiquitos a “patrimony of humanity”. Dr. Gustavo Leone of Loyola University Chicago\u27s Department of Fine and Performing Arts has painstakingly retrieved and restored several of these incredible manuscripts, preserving a rare and invaluable treasure of the Jesuit and Catholic heritage. This is the first publication of this anonymous composition, collected at the Music Archives of the Chiquitos, in Concepción, Bolivia. This composition is attributed to Fr. Martin Schmid, SJ. This composition, Regina Caeli Laetare, is for SATB choir, violin 1, violin 1, and continuo. Funded in part by a faculty research grant from the Joan and Bill Hank Center for the Catholic Intellectual Heritage at Loyola University Chicago. The Regina Caeli or Regina Coeli ( Queen of Heaven ), an ancient Latin Marian Hymn of the Christian Church, is one of the four seasonal Marian antiphons of the Blessed Virgin Mary, prescribed to be sung or recited in the Liturgy of the Hours at the conclusion of the last of the hours to be prayed in common that day, typically night prayer (Compline or Vespers). This score and instrumental parts are available for performance by downloading them from this site for free

Thermal Field Theory and Generalized Light Front Coordinates

The dependence of thermal field theory on the surface of quantization and on the velocity of the heat bath is investigated by working in general coordinates that are arbitrary linear combinations of the Minkowski coordinates. In the general coordinates the metric tensor $g_{\bar{\mu\nu}}$ is non-diagonal. The Kubo, Martin, Schwinger condition requires periodicity in thermal correlation functions when the temporal variable changes by an amount $-i\big/(T\sqrt{g_{\bar{00}}})$. Light front quantization fails since $g_{\bar{00}}=0$, however various related quantizations are possible.Comment: 10 page

Stress and food deprivation: linking physiological state to migration success in a teleost fish

Food deprivation is a naturally occurring stressor that is thought to influence the ultimate life-history strategy of individuals. Little is known about how food deprivation interacts with other stressors to influence migration success. European populations of brown trout (Salmo trutta) exhibit partial migration, whereby a portion of the population smoltifies and migrates to the ocean, and the rest remain in their natal stream. This distinct, natural dichotomy of life-history strategies provides an excellent opportunity to explore the roles of energetic state (as affected by food deprivation) and activation of the glucocorticoid stress response in determining life-history strategy and survival of a migratory species. Using an experimental approach, the relative influences of short-term food deprivation and experimental cortisol elevation (i.e. intra-coelomic injection of cortisol suspended in cocoa butter) on migratory status, survival and growth of juvenile brown trout relative to a control were evaluated. Fewer fish migrated in both the food deprivation and cortisol treatments; however, migration of fish in cortisol and control treatments occurred at the same time while that of fish in the food deprivation treatment was delayed for approximately 1 week. A significantly greater proportion of trout in the food deprivation treatment remained in their natal stream, but unlike the cortisol treatment, there were no long-term negative effects of food deprivation on growth, relative to the control. Overall survival rates were comparable between the food deprivation and control treatments, but significantly lower for fish in the cortisol treatment. Food availability and individual energetic state appear to dictate the future life-history strategy (migrate or remain resident) of juvenile salmonids while experimental elevation of the stress hormone cortisol causes impaired growth and reduced survival of both resident and migratory individuals