Direct Profiling the Post-Translational Modification Codes of a Single Protein Immobilized on a Surface Using Cu-free Click Chemistry

Combinatorial post-translational modifications (PTMs), which can serve as dynamic molecular barcodes, have been proposed to regulate distinct protein functions. However, studies of combinatorial PTMs on single protein molecules have been hindered by a lack of suitable analytical methods. Here, we describe erasable single-molecule blotting (eSiMBlot) for combinatorial PTM profiling. This assay is performed in a highly multiplexed manner and leverages the benefits of covalent protein immobilization, cyclic probing with different antibodies, and single molecule fluorescence imaging. Especially, facile and efficient covalent immobilization on a surface using Cu-free click chemistry permits multiple rounds (>10) of antibody erasing/reprobing without loss of antigenicity. Moreover, cumulative detection of coregistered multiple data sets for immobilized single-epitope molecules, such as HA peptide, can be used to increase the antibody detection rate. Finally, eSiMBlot enables direct visualization and quantitative profiling of combinatorial PTM codes at the single-molecule level, as we demonstrate by revealing the novel phospho-codes of ligand-induced epidermal growth factor receptor. Thus, eSiMBlot provides an unprecedentedly simple, rapid, and versatile platform for analyzing the vast number of combinatorial PTMs in biological pathways. Copyright © 2018 American Chemical Societ

Effects of different three live foods on growth performance and survival rates in beluga (Huso huso) larvae

To determine the best live food and to reevaluate the optimal weaning period for beluga fish (Huso huso) larvae, seven experimental diets: Daphina (DP), Chironomids (CH), Gammarid (G), Daphina + formulated diet (DPFD), Chironomids + formulated diet (CHFD), Gammarid + formulated diet (GFD ) and formulated diet (FD) in triplicate groups were fed to 4662 sixteen-days-old larvae which were captured from the stock tank and randomly distributed into 21 tanks. The microdiets contained 48-50% crude protein, 12-13% crude fat, 9-11% moisture and 8.5-9.5% ash . After 25 days feeding trial, weight gain (WG), specific growth rate (SGR) and feed efficiency (FE) of fish fed CHFD were significantly higher than those of fish fed the other diets ( P< 0.05). Frequent cannibalism and higher mortality in larvae fed G, GFD and FD diets were observed. These results may show the importance of live food followed by formulated diets in a gradual application pattern, as early as on the fourth to fifth day after hatching

Base-treated juniper fiber media for removing heavy metals in stormwater runoff

The viability of base-treated juniper fiber (BTJF) media for removing toxic heavy metals (Cd2+, Cu2-, Pb2+, Zn2+) in stormwater runoff was investigated. The sorption ability of the BTJF for all metals was much higher than that of untreated juniper. The affinity sequence of both materials, BTJF and untreated juniper, was Pb&gt; Cu&gt; Zn≥ Cd. This order is explained by the hydrolysis constants for each metal. A metal desorption and column regeneration test using 0. 1 M nitric acid showed that the metal sorption capacity declined slightly from 136.3 to 119.2 μmole/g in the first two cycles and then more significantly at the third and fourth regeneration, 72.3 and 83.1 μmole/g, respectively. Based on the hydraulic conductivity test of BTJF of different size classes, it can be deduced that there is no major headloss-related disadvantage in using BTJF instead of sand as stormwater filter media if the particle size of the BTJF is similar to that for sand

Hydrous ferric oxide incorporated diatomite for remediation of arsenic contaminated groundwater

Two reactive media zerovalent iron (ZVI, Fisher Fe0) and amorphous hydrous ferric oxide (HFO)-incorporated porous, naturally occurring aluminum silicate diatomite designated as Fe (25)-diatomite]], were tested for batch kinetic, pH-controlled differential column batch reactors (DCBRs), in small- and large-scale column tests (about 50 and 900 mL of bed volume) with groundwater from a hazardous waste site containing high concentrations of arsenic (both organic and inorganic species), as well as other toxic or carcinogenic volatile and semivolatile organic compounds (VOC/SVOCs). Granular activated carbon (GAC) was also included as a reactive media since a permeable reactive barrier (PRB) at the subject site would need to address the hazardous VOC/SVOC contamination as well as arsenic. The groundwater contained an extremely high arsenic concentration (341 mg L-1) and the results of ion chromatography and inductively coupled plasma mass spectrometry (IC-ICP-MS) analysis showed that the dominant arsenic species were arsenite (45.1) and monomethyl arsenic acid (MMAA, 22.7), while dimethyl arsenic acid (DMAA) and arsenate were only 2.4 and 1.3, respectively. Based on these proportions of arsenic species and the initial As-to-Fe molar ratio (0.15 molAs molFe-1), batch kinetic tests revealed that the sorption density (0.076 molAs molFe-1) for Fe (25)-diatomite seems to be less than the expected value (0.086 molAs molFe-1) calculated from the sorption density data reported by Lafferty and Loeppert (Environ. Sci. Technol. 2005, 39, 2120-2127), implying that natural organic matters (NOMs) might play a significant role in reducing arsenic removal efficiency. The results of pH-controlled DCBR tests using different synthetic species of arsenic solution showed that the humic acid inhibited the MMAA removal of Fe (25)-diatomite more than arsenite. The mixed system of GAC and Fe (25)-diatomite increased the arsenic sorption speed to more than that of either individual media alone. This increase might be deduced by the fact that the addition of GAC could enhance arsenic removal performance of Fe (25)-diatomite through removing comparably high portions of NOMs. Small- and large-scale column studies demonstrated that the empty bed contact time (EBCT) significantly affected sorpton capacities at breakthrough (C = 0.5 C 0) for the Fe0/sand (50/50, w/w) mixture, but not for GAC preloaded Fe (25)-diatomite. In the large-scale column tests with actual groundwater conditions, the GAC preloaded Fe (25)-diatomite effectively reduced arsenic to below 50 μg L-1 for 44 days; additionally, most species of VOC/SVOCs were also simultaneously attenuated to levels below detection. © 2007 American Chemical Society

BSS Plus compared to the vitreous of non-diabetics and diabetics

Letter to the EditorAbstract not availableJan Kokavec, Saban Horo, Weng Onn Chan, San H Min, Mei H Tan, John Grigg, Jagjit S Gilhotra, Henry S Newland, Shane R Durkin and Robert J Casso

Cost-effectiveness of all-oral regimens for the treatment of multidrug-resistant tuberculosis in Korea: comparison with conventional injectable-containing regimens

Background Regimens for the treatment of multidrug-resistant tuberculosis (MDR-TB) have been changed from injectable-containing regimens to all-oral regimens. The economic effectiveness of new all-oral regimens compared with conventional injectable-containing regimens was scarcely evaluated. This study was conducted to compare the cost-effectiveness between all-oral longer-course regimens (the oral regimen group) and conventional injectable-containing regimens (the control group) to treat newly diagnosed MDR-TB patients. Methods A health economic analysis over lifetime horizon (20 years) from the perspective of the healthcare system in Korea was conducted. We developed a combined simulation model of a decision tree model (initial two years) and two Markov models (remaining 18 years, six-month cycle length) to calculate the incremental cost-effectiveness ratio (ICER) between the two groups. The transition probabilities and cost in each cycle were assumed based on the published data and the analysis of health big data that combined country-level claims data and TB registry in 2013–2018. Results: The oral regimen group was assumed to spend 20,778 USD more and lived 1.093 years or 1.056 quality-adjusted life year (QALY) longer than the control group. The ICER of the base case was calculated to be 19,007 USD/life year gained and 19,674 USD/QALY. The results of sensitivity analyses showed that base case results were very robust and stable, and the oral regimen was cost-effective with a 100% probability for a willingness to pay more than 21,250 USD/QALY. Conclusion This study confirmed that the new all-oral longer regimens for the treatment of MDR-TB were cost-effective in replacing conventional injectable-containing regimens

Synthesis, structure and magnetic properties ofβ-MnO2nanorods

We present synthesis, structure and magnetic properties of structurally well-ordered single-crystalline β-MnO2nanorods of 50–100 nm diameter and several µm length. Thorough structural characterization shows that the basic β-MnO2material is covered by a thin surface layer (∼2.5 nm) of α-Mn2O3phase with a reduced Mn valence that adds its own magnetic signal to the total magnetization of the β-MnO2nanorods. The relatively complicated temperature-dependent magnetism of the nanorods can be explained in terms of a superposition of bulk magnetic properties of spatially segregated β-MnO2and α-Mn2O3constituent phases and the soft ferromagnetism of the thin interface layer between these two phases

Parametrization of nonlinear and chaotic oscillations in driven beam-plasma diodes

Nonlinear phenomena in a driven plasma diode are studied using a fluid code and the particle-in-cell simulation code XPDPI. When a uniform electron beam is injected to a bounded diode filled with uniform ion background, the beam is destabilized by the Pierce instability and a perturbation grows to exhibit nonlinear oscillations including chaos. Two standard routes to chaos, period doubling and quasiperiodicity, are observed. Mode lockings of various winding numbers are observed in an ac driven system. A new diagnostic quantity is used to parametrize various nonlinear oscillations.open10