551 research outputs found
OsSFR6 is a functional rice orthologue of SENSITIVE TO FREEZING-6 and can act as a regulator of COR gene expression, osmotic stress and freezing tolerance in Arabidopsis
The Arabidopsis protein SENSITIVE TO FREEZING-6 (AtSFR6) is required for cold- and drought-inducible expression of COLD-ON REGULATED (COR) genes and, as a consequence, AtSFR6 is essential for osmotic stress and freezing tolerance in Arabidopsis. Therefore, orthologues of AtSFR6 in crop species represent important candidate targets for future manipulation of stress tolerance. We identified and cloned a homologue of AtSFR6 from rice (Oryza sativa), OsSFR6, and confirmed its orthology in Arabidopsis. OsSFR6 was identified by homology searches, and a full-length coding region isolated using reverse transcription polymerase chain reaction (RT-PCR) from Oryza sativa cDNA. To test for orthology, OsSFR6 was expressed in an Arabidopsis sfr6 loss-of-function mutant background, and restoration of wild-type phenotypes was assessed. Searching the rice genome revealed a single homologue of AtSFR6. Cloning and sequencing the OsSFR6 coding region showed OsSFR6 to have 61.7% identity and 71.1% similarity to AtSFR6 at the predicted protein sequence level. Expression of OsSFR6 in the atsfr6 mutant background restored the wild-type visible phenotype, as well as restoring wild-type levels of COR gene expression and tolerance of osmotic and freezing stresses. OsSFR6 is an orthologue of AtSFR6, and thus a target for future manipulation to improve tolerance to osmotic and other abiotic stresses
Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts
Various methods are currently used to investigate human tissue differentiation, including human embryo culture and studies utilising pluripotent stem cells (PSCs) such as in vitro embryoid body formation and in vivo teratoma assays. Each method has its own distinct advantages, yet many are limited due to being unable to achieve the complexity and maturity of tissue structures observed in the developed human. The teratoma xenograft assay allows maturation of more complex tissue derivatives, but this method has ethical issues surrounding animal usage and significant protocol variation. In this study, we have combined three-dimensional (3D) in vitro cell technologies including the common technique of embryoid body (EB) formation with a novel porous scaffold membrane, in order to prolong cell viability and extend the differentiation of PSC derived EBs. This approach enables the formation of more complex morphologically identifiable 3D tissue structures representative of all three primary germ layers. Preliminary in vitro work with the human embryonal carcinoma line TERA2.SP12 demonstrated improved EB viability and enhanced tissue structure formation, comparable to teratocarcinoma xenografts derived in vivo from the same cell line. This is thought to be due to reduced diffusion distances as the shape of the spherical EB transforms and flattens, allowing for improved nutritional/oxygen support to the developing structures over extended periods. Further work with EBs derived from murine embryonic stem cells demonstrated that the formation of a wide range of complex, recognisable tissue structures could be achieved within 2-3 weeks of culture. Rudimentary tissue structures from all three germ layers were present, including epidermal, cartilage and epithelial tissues, again, strongly resembling tissue structure of teratoma xenografts of the same cell line. Proof of concept work with EBs derived from the human embryonic stem cell line H9 also showed the ability to form complex tissue structures within this system. This novel yet simple model offers a controllable, reproducible method to achieve complex tissue formation in vitro. It has the potential to be used to study human developmental processes, as well as offering an animal free alternative method to the teratoma assay to assess the developmental potential of novel stem cell lines
Generalized drift-diffusion model for miniband superlattices
A drift-diffusion model of miniband transport in strongly coupled
superlattices is derived from the single-miniband Boltzmann-Poisson transport
equation with a BGK (Bhatnagar-Gross-Krook) collision term. We use a consistent
Chapman-Enskog method to analyze the hyperbolic limit, at which collision and
electric field terms dominate the other terms in the Boltzmann equation. The
reduced equation is of the drift-diffusion type, but it includes additional
terms, and diffusion and drift do not obey the Einstein relation except in the
limit of high temperatures.Comment: 4 pages, 3 figures, double-column revtex. To appear as RC in PR
Motion-Induced Radiation from a Dynamically Deforming Mirror
A path integral formulation is developed to study the spectrum of radiation
from a perfectly reflecting (conducting) surface. It allows us to study
arbitrary deformations in space and time. The spectrum is calculated to second
order in the height function. For a harmonic traveling wave on the surface, we
find many different regimes in which the radiation is restricted to certain
directions. It is shown that high frequency photons are emitted in a beam with
relatively low angular dispersion whose direction can be controlled by the
mechanical deformations of the plate.Comment: 4 pages, 2 eps figues included, final version as appeared in PR
The Role of Friction in Compaction and Segregation of Granular Materials
We investigate the role of friction in compaction and segregation of granular
materials by combining Edwards' thermodynamic hypothesis with a simple
mechanical model and mean-field based geometrical calculations. Systems of
single species with large friction coefficients are found to compact less.
Binary mixtures of grains differing in frictional properties are found to
segregate at high compactivities, in contrary to granular mixtures differing in
size, which segregate at low compactivities. A phase diagram for segregation
vs. friction coefficients of the two species is generated. Finally, the
characteristics of segregation are related directly to the volume fraction
without the explicit use of the yet unclear notion of compactivity.Comment: 9 pages, 6 figures, submitted to Phys. Rev.
Full quantum solutions to the resonant four-wave mixing of two single-photon wave packets
We analyze both analytically and numerically the resonant four-wave mixing of
two co-propagating single-photon wave packets. We present analytic expressions
for the two-photon wave function and show that soliton-type quantum solutions
exist which display a shape-preserving oscillatory exchange of excitations
between the modes. Potential applications including quantum information
processing are discussed.Comment: 7 pages, 3 figure
Comparative evaluation of a new lactation curve model for pasture-based Holstein-Friesian dairy cows
Giant pulses of pulsar radio emission
Review report of giant pulses of pulsar radio emission, based on our
detections of four new pulsars with giant pulses, and the comparative analysis
of the previously known pulsars with giant pulses, including the Crab pulsar
and millisecond pulsar PSR B1937+21.Comment: Proceedings of the 363. WE-Heraeus Seminar on: Neutron Stars and
Pulsars (Posters and contributed talks) Physikzentrum Bad Honnef, Germany,
May.14-19, 2006, eds. W.Becker, H.H.Huang, MPE Report 291, pp.72-7
Ionic and electronic structure of sodium clusters up to N=59
We determined the ionic and electronic structure of sodium clusters with even
electron numbers and 2 to 59 atoms in axially averaged and three-dimensional
density functional calculations. A local, phenomenological pseudopotential that
reproduces important bulk and atomic properties and facilitates structure
calculations has been developed. Photoabsorption spectra have been calculated
for , , and to
. The consistent inclusion of ionic structure considerably
improves agreement with experiment. An icosahedral growth pattern is observed
for to . This finding is supported by
photoabsorption data.Comment: To appear in Phys. Rev. B 62. Version with figures in better quality
can be requested from the author
Electric current circuits in astrophysics
Cosmic magnetic structures have in common that they are anchored
in a dynamo, that an external driver converts kinetic energy into internal
magnetic energy, that this magnetic energy is transported as Poynting fl ux across the magnetically dominated structure, and that the magnetic energy
is released in the form of particle acceleration, heating, bulk motion,
MHD waves, and radiation. The investigation of the electric current system is
particularly illuminating as to the course of events and the physics involved.
We demonstrate this for the radio pulsar wind, the solar flare, and terrestrial
magnetic storms
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