A STUDY ON SEROPREVELENCE AND RISK FACTORS OF BOVINE LEPTOSPIROSIS IN LOWER ASSAM, INDIA

The present study was conducted to ascertain the seroprevalence of Leptospira infection in cattle among certain districts of the lower Brahmaputra valley in the state of Assam from March 2017 to February 2018. Two serological tests viz. IgG ELISA and Microscopic Agglutination Test (MAT) were used to detect the leptospiral antibodies. The anti-leptospiral antibodies were detected by IgG ELISA and compared with MAT using antigens from 12 pathogenic serovars. Out of 380 sera samples, 68 (17.89 %, 95% CI: 6.89-27) and 44 (11.58%) were positive by IgG ELISA and MAT respectively. The circulating Leptospira serovars identified were Autumnalis (6.05%), Ballum (2.63%), Batavia (1.31%), Ichterohaemorrhagie (0.7%), Javanica (0.5%) and Sejroe (0.2%). The sensitivity and specificity of IgG ELISA in comparison to MAT were calculated and found to be 100% and 92.85% respectively with a concordance of 93.68%. An epidemiological investigation was carried out to find the association of various risk factors with Leptospira infection under this study in cattle using a pre-tested questionnaire. The present study will serve as baseline data for the prevention and control of Leptospira infection in cattle

Isolation of Salmonella Typhimurium from poultry eggs and meat of Tarai region of Uttaranchal

407-409Presence of Salmonella in samples, 100 each of chicken eggs and meat, collected from various retail outlets of the Tarai region of Uttaranchal, was detected by polymerase chain reaction (PCR) using InvA gene primer, a rapid and sensitive DNA based method. The results were further validated by established conventional method of Salmonella isolation and biochemical tests, including slide agglutination test (poly “O” antiserum specific for Salmonella). Of 200 samples analyzed, 4 (one meat and 3 egg) were found to be contaminated with Salmonella enterica var Typhimurium. Further, the antibiogram of the isolates revealed sensitivity in the decreasing order to Chloromphenicol, Colistin, Polymixin, Enrofloxacin, and Ciprofloxacin

The extent and structure of pig rearing system in urban and peri-urban areas of Guwahati

Livestock is common in Indian cities and contribute to food security as well as livelihoods. Urban livestock keeping has been neglected, and in India, little is known about the topic. Therefore, urban and peri-urban pig farms of Guwahati, Assam, India, were surveyed in order to understand more about the pig rearing systems and risks of diseases. A total of 34 urban and 66 peri-urbanpig farms were selected randomly. All reared cross-bred pigs. Free-range pig rearing was common in both urban (58.8%) and peri-urban (45.45%) farms. Artificial insemination was used by around half of the pig farmers. Disinfection in pig farms was practiced in 26.5% of urban and 28.8% of peri-urban farms. More urban pig farms were observed to be moderately clean in (82.4%) compared to peri-urban (69.7%). However, more urban (67.7%) than peri-urban farms (57.6%) reported ahighrodent burden. Pig sheds were mostly basic, with bricked floors in 18.2% farms in peri-urban areas, and more than 80% had corrugated iron roofing sheets. In conclusion, free-roaming pigs in both urban and peri-urban areas of Guwahati can contribute to disease transmission, and the low standard of hygiene and buildings may further increase the risk of diseases

Green Tea Leaves Extract with Low Concentration of EGCG Can Provide Health Benefits without Causing Renal Damage

Green tea or its concentrated extract is coveted for its health promoting catechin-like polyphenols, especially epigallocatechin-3-gallate (EGCG). However, its amicable efficacy is now being doubted considering the recent occurrence of several cases of hepato- and nephrotoxicity, after the ingestion of EGCG-fortified (≥85–90%) nutritional supplements. Therefore, the current study was carried out to ascertain the effect of green tea leaves extract (GTE), having low EGCG content (73.8%), on liver and kidney functions of male Wistar rats using various in vivo experiments and in vitro radical scavenging activity. In terms of acute toxicity, GTE was observed to be safe when delivered at a dosage of 2000 mg kg-1 body weight (BW). Oral delivery of GTE for 28 days at a concentration of 200 mg kg-1 BW/day did not trigger sub-acute toxicity to the liver and kidneys, as per serum biochemical analyses and histopathological examination. In contrast, GTE counteracted the effects of carbon tetrachloride (a potent hepato-degenerative compound) on the liver. Furthermore, increase in high-density lipoprotein-cholesterol with concomitant lowering of serum triglycerides and low-density lipoprotein-cholesterol were noticed in GTE-treated rats. These findings suggest that low EGCG containing GTE, with appreciable antioxidant activity (IC50 = 53.18–71.28 µgmL-1), can serve as a hepatoprotective, hypolipidemic, and hypocholesterolemic ingredient

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Not AvailableThe Arunachali breed of yak is reared by the Monpa pastoral community of Arunachal Pradesh in India for their livelihood and nutritional security on highland pastures. In addition to fulfilling the basic needs of highlanders, this animal is associated with poor production potential linked to its inherent reproductive problems, including silent oestrus, seasonality, and inbreeding, making yak rearing a less-profitable venture. In the recent past, efforts have been made to improve reproductive efficiency of Arunachali yak through assisted reproductive techniques. However, a few regimens for superovulation have been tried to date with limited success in terms of superovulatory response and embryo recovery. Therefore, the present study was undertaken to evaluate the efficacy of two different doses and regimes of superovulation: Stimufol (400 and 200 µg per animal) and Folligon (1500 and 1000 IU per animal) in Arunachali yak. Twenty-four yaks were allotted in 4 groups (of 6 animals each) and subjected to respective superovulation treatment. Thereafter, the treatment response was assessed in terms of expression of oestrus, interval between treatment and oestrus onset, duration of oestrous, number of corpora lutea and embryos recovered, and analysed using two-way statistical analysis between treatment groups. The results (Table 1) showed a significant (P < 0.01) variation in onset of oestrus after the treatment, whereas other responses did not differ among the groups. Further, the recovered embryos were categorized as excellent, good, fair, and poor according to cell size, shape, texture, and number of vesicles present in the blastomeres; 58.33, 20.83, 8.33, and 12.5% embryos in these categories were recovered, respectively. The overall embryo recovery rate was 30.77%, ranging from 10.53 to 52.38% in different treatment groups. The numbers of embryos recorded were less than the numbers of CL present, showing the possibility of non-availability of all ovulated oocytes for fertilization in the oviduct due to untrapping by the fimbriae. The effectiveness of treatments depended upon the precision of oestrus detection and the time of ovulation. In our study, the variation in onset of oestrus might be associated with poor expression of behavioural oestrus in the treated animals. Based on the present findings, we conclude that all the treatments are effective in superovulation and embryo recovery. However, the use of Folligon at 1500 IU seemed to be comparatively more effective in Arunachali yak.Not Availabl

Catechin and Catechin Fractions as Biochemical Markers to Study the Diversity of Indian Tea (Camellia sinensis (L.) O. KUNTZE) Germplasm

The heterogeneous Indian tea germplasm includes 'China', 'Assam', 'Cambod', and their hybrids which were evaluated using biochemical markers viz., total catechin and their fractions, for varietal identification and characterization. Principal component analysis (PCA) of biochemical characters showed that the total catechin and trihydroxylated catechin has higher eigenvalues. The first two principal components (PCs) could differentiate more than 90% of the clones studied. This grouping based on first two principal component matrices differentiated 'China', and their hybrids with 'Assam' and 'Cambod' variety. Morphologically indistinct large-leaved 'Cambod' variety and 'Assam' varieties could not be differentiated using biochemical markers, since both varietal types taxonomically belong to a single species. Clones of 'China' type showed low total catechin content and catechin ratio which are distinctly grouped. The 'China-Assam' and 'China-Cambod' hybrids formed intermediate groups between 'China' PC group and 'Cambod'/'Assam' PC groups, providing evidence for genetic control of catechin ratio variation. Tea clones which are differentially positioned in the PC group could be explained based on the genetic contribution by other varietal type as parents. This biochemical characterization will be a useful tool in the development of quality-tea clones with different proportion of total catechin and their fractions

A GMMA-CPS-Based Vaccine for Non-Typhoidal <i>Salmonella</i>

Non-typhoidal Salmonella are a major cause of gastroenteritis worldwide, as well as causing bloodstream infections in sub-Saharan Africa with a high fatality rate. No vaccine is currently available for human use. Current vaccine development strategies are focused on capsular polysaccharides (CPS) present on the surface of non-typhoidal Salmonella. This study aimed to boost the amount of CPS purified from S. Typhimurium for immunization trials. Random mutagenesis with Tn10 transposon increased the production of CPS colanic acid, by 10-fold compared to wildtype. Immunization with colanic acid or colanic acid conjugated to truncated glycoprotein D or inactivated diphtheria toxin did not induce a protective immune response in mice. However, immunization with Generalized Modules for Membrane Antigens (GMMAs) isolated from colanic acid overproducing isolates reduced Salmonella colonization in mice. Our results support the development of a GMMA-CPS-based vaccine against non-typhoidal Salmonella