Case report: a unique pediatric case of a primary CD8 expressing ALK-1 positive anaplastic large cell lymphoma of skeletal muscle

Primary involvement of skeletal muscle is a very rare event in ALK-1 positive anaplastic large cell lymphoma (ALCL). We describe a case of a 10-year old boy presenting with a three week history of pain and a palpable firm swelling at the dorsal aspect of the left thigh. Histological examination of the lesion revealed a tumoral and diffuse polymorphic infiltration of the muscle by large lymphoid cells. Tumor cells displayed eccentric, lobulated "horse shoe" or "kidney-shape" nuclei. The cells showed immunohistochemical positivity for CD30, ALK-1, CD2, CD3, CD7, CD8, and Perforin. Fluorescence in situ hybridization analysis revealed a characteristic rearrangement of the ALK-1 gene in 2p23 leading to the diagnosis of ALK-1 positive ALCL. Chemotherapy according to the ALCL-99-NHL-BFM protocol was initiated and resulted in a complete remission after two cycles. This case illustrates the unusual presentation of a pediatric ALCL in soft tissue with a good response to chemotherapy

Precursor lesions of early onset pancreatic cancer

Early onset pancreatic cancer (EOPC) constitutes less than 5% of all newly diagnosed cases of pancreatic cancer (PC). Although histopathological characteristics of EOPC have been described, no detailed reports on precursor lesions of EOPC are available. In the present study, we aimed to describe histopathological picture of extratumoral parenchyma in 23 cases of EOPCs (definition based on the threshold value of 45 years of age) with particular emphasis on two types of precursor lesions of PC: pancreatic intraepithelial neoplasia (PanIN) and intraductal papillary mucinous neoplasms (IPMNs). The types, grades, and densities of precursor lesions of PC were compared in patients with EOPCs, in young patients with neuroendocrine neoplasms (NENs), and in older (at the age of 46 or more) patients with PC. PanINs were found in 95.6% of cases of EOPCs. PanINs-3 were found in 39.1% of EOPC cases. Densities of all PanIN grades in EOPC cases were larger than in young patients with NENs. Density of PanINs-1A in EOPC cases was larger than in older patients with PC, but densities of PanINs of other grades were comparable. IPMN was found only in a single patient with EOPC but in 20% of older patients with PC. PanINs are the most prevalent precursor lesions of EOPC. IPMNs are rarely precursor lesions of EOPC. Relatively high density of low-grade PanINs-1 in extratumoral parenchyma of patients with EOPC may result from unknown multifocal genetic alterations in pancreatic tissue in patients with EOPCs

Resistance to antimicrobials in poultry production chain isolated bacteria

Bacterial resistance to antimicrobials is a global problem. A possible cause of the emergence of resistance\nis their use as growth promoters. This can cause therapeutic failures and increase the transmission of\nresistant bacteria. The objective of this work was to determine the sensitivity to antimicrobials of isolated\nbacteria from poultry and products for human consumption. Sixty samples were studied and Escherichia\ncoli, Salmonella spp. and Enterococcus faecalis were isolated. Around 80 % of the farm samples showed\ncounts of >103 UFC/g for E. coli and 95 % for E. faecalis; 70 % of the refrigerated samples showed counts\nof >103 UFC/g for E. coli, and 85 % of the market samples showed counts of > 103 CFU /g for E. faecalis, 15 % for E. coli; and 5 % developed Salmonella spp. Of the 53 E. coli isolations, 47.1 % were resistant to fluoroquinolones, 9.4 % to third-generation cephalosporins, and 3.8 % to colistin. Of the 39 E. faecalis isolated strains, one was resistant to ciprofloxacin, and none to vancomycin. The isolated Salmonella spp. was resistant to ciprofloxacin. Our results reveal the need to intensify sanitary and hygiene controls poultry manipulation chain and implement improvements to pathogens reduction.Fil: López, V. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Tandil, ArgentinaFil: Guerriero, L. Fares Taie Instituto de Análisis. Mar del Plata, ArgentinaFil: Elorza, V. Fares Taie Instituto de Análisis. Mar del Plata, ArgentinaFil: Krüger, A. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Tandil, ArgentinaFil: Krüger, A. CONICET. Centro de Investigación Veterinaria de Tandil (CIVETAN). Tandil, ArgentinaFil: Colello, R. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Tandil, ArgentinaFil: Colello, R. CONICET. Centro de Investigación Veterinaria de Tandil (CIVETAN). Tandil, ArgentinaFil: Medici, S. Centro de Alimentos y Medio Ambiente Fares Taie Instituto de Análisis. Mar del Plata, ArgentinaFil: Espinosa, M. Centro de Alimentos y Medio Ambiente Fares Taie Instituto de Análisis. Mar del Plata, ArgentinaFil: Casado, P. Centro de Alimentos y Medio Ambiente Fares Taie Instituto de Análisis. Mar del Plata, ArgentinaFil: Recavarren, M. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Tandil, ArgentinaFil: Recavarren, M. Fares Taie Instituto de Análisis. Mar del Plata, ArgentinaFil: Keller, L. Fares Taie Instituto de Análisis. Mar del Plata, ArgentinaLa resistencia bacteriana a los antimicrobianos es un problema global. Una posible causa de aparición de\nresistencia es su uso como promotores de crecimiento en aves. Esto puede producir fallos terapéuticos e\nincrementar la transmisión de bacterias resistentes al hombre. El objetivo de este trabajo fue determinar\nla sensibilidad a antimicrobianos en bacterias aisladas de aves y subproductos para consumo humano. Se\nestudiaron 60 muestras, aislándose Escherichia coli, Salmonella spp. y Enterococcus faecalis. Un 80 % de las muestras de granja presentaron recuentos >103 UFC/g para E. coli y 95 % para E. faecalis; 70 % de las de frigorífico tuvieron recuentos >103 UFC/g de E. coli y 85 % de las de supermercado presentaron recuentos\n>103 UFC/g de E. faecalis, 15% de E. coli; y 5 % presentó desarrollo de Salmonella spp. De 53 aislamientos de E. coli, 47,1% fueron resistentes a fluoroquinolonas, 9,4 % resistentes a cefalosporinas de tercera generación, y 3,8 % a colistina. De 39 cepas de E. faecalis, una fue resistente a ciprofloxacina, y ninguna a\nvancomicina. El aislamiento de Salmonella spp. fue resistente a ciprofloxacina. Nuestros resultados revelarían la necesidad de intensificar los controles higiénico-sanitarios en la cadena de manipulación de aves, e implementar mejoras para la reducción de patógenos