Cloning and expression of Aujeszky's disease virus glycoprotein E (gE) in a baculovirus system.

Projeto n. 03.03.23.20

Dermatophylosis in Santa Inês sheep from Distrito Federal

Relataram-se quatro casos de dermatofilose em ovinos da raça Santa Inês, no período de um ano. Microscopicamente observaram-se filamentos na forma de "trilho de bonde" e zoósporos nos quatro casos. A tentativa do isolamento do microrganismo foi realizada por meio do método de Haalstra e em apenas um caso obteve-se sucesso, observando-se colônias de aparência lisa, formato circular, cor amarelada e hemolítica em ágar sangue. O exame direto com coloração de Gram mostrou-se um método bastante eficiente na confirmação da presença do microrganismo afetando a epiderme em razão da morfologia típica do agente.Four cases of dermatophylosis were reported in Santa Inês sheep in a study period of one year. Microscopically, septate filaments and coccoid forms zoospores were observed. Attempts to isolate the microorganisms were accomplished using Haastra's method and it was successful in only one case. Dermatophilus congolensis samples have grown on blood agar, colonies where hemolytic, small, round and pigmentation vary from yellow to orange. The gram staining method was efficient to confirm the presence of the microorganism affecting the epidermis due to typical morphology of the agent

Genes de enterotoxinas e perfil antimicrobiano de Escherichia coli isoladas de suínos hígidos no Distrito Federal

Um total de 127 cepas de Escherichia coli foi isolado de suínos no Distrito Federal, testado para a presença de genes de enterotoxinas (STa, LT-I, LT-II, Stx1 e Stx2) e para resistência antimicrobiana. Das cepas isoladas, oito (6,3%) possuíam genes para enterotoxinas, sendo quatro (3,2%) positivas somente para LT-I, três (2,4%) somente para STa e uma (0,8%) positiva para STa e LT-I. Nenhuma das cepas isoladas apresentou genes para LT-II, Stx1 ou Stx2. Quanto ao perfil de resistência antimicrobiano, os antibióticos com maiores porcentagens de resistência foram lincomicina (100%), sulfonamidas (74,8%) e tetraciclina (70,1%), enquanto os maiores índices de sensibilidade foram observados na norfloxacina (82,7%), gentamicina (75,6%) e sulfametoxazol + trimetoprim (63%). Esses resultados demonstraram a presença de genes de enterotoxinas e altas taxas de resistência antimicrobiana em E. coli isoladas de suínos hígidos no DF

Seroprevalence of hepatitis E virus infection in domestic pigs in the Federal District, Brazil

ABSTRACT Hepatitis E is caused by the hepatitis E virus (HEV) which is currently known to be a zoonotic pathogen transmitted by pigs. In Brazil, there is no information about the circulation of HEV in the swine herd of the Federal District. Therefore, a cross-sectional study was performed with sera from 449 domestic pigs, provided by the Secretary of Agriculture of the Federal District. Blood samples were collected between June and September 2014. A commercially available ELISA kit was used for the detection of IgG antibodies. High seroprevalence of antibodies to HEV was found, since 304 animals showed anti-HEV positive reactions (67.7%; 95% CI = 63.2%, 71.9%). The seropositivity presented no difference by gender or age. The results suggest that HEV circulates among domestic pigs in the Federal District and it can serve as a warning to the local public health system due to their possible involvement in human infections

Antimicrobial resistance of bacterial strains isolated from avian cellulitis

Avian cellulitis is an inflammatory process in the subcutaneous tissue, mainly located in the abdomen and thighs. This problem is commonly observed in poultry at slaughter and it is considered one of the major causes of condemnation of carcasses in Brazil. The aim of this study was to perform the microbial isolation of lesions of avian cellulitis from a processing plant located in the State of Goiás in order to analyze antimicrobial resistance by antibiogram test and to detect resistance genes by polymerase chain reaction. A total of 25 samples of avian cellulitis lesions were analyzed, from which 30 bacterial strains were isolated. There were eleven (44%) strains of Escherichia coli, nine (36%) strains of Staphylococcus epidermidis, seven (28%) strains of Proteus mirabilis and three (12%) strains of Manheimiahaemolytica. The antibiogram test showed that all strains were resistant to at least one antimicrobial. The gene of antimicrobial resistance tetB was detected in E. coli, S. epidermidis and P. mirabilis strains, and was the most frequently observed gene. The gene of antimicrobial resistance Sul1 was detected in all bacterial species, while tetA was found in E. coli and S. epidermidis strains, SHV in E. coli strains, S. epidermidis and P. mirabilis,and cat1 in one P. mirabilis strain. The results suggest a potential public health hazard due to the ability of these microorganisms to transmit antimicrobial resistancegenes to other microorganisms present in the intestinal tract of humans and animals, which may affect clinical-medical usage of these drugs

Seroprevalence of hepatitis E virus infection in domestic pigs in the Federal District, Brazil

<div><p>ABSTRACT Hepatitis E is caused by the hepatitis E virus (HEV) which is currently known to be a zoonotic pathogen transmitted by pigs. In Brazil, there is no information about the circulation of HEV in the swine herd of the Federal District. Therefore, a cross-sectional study was performed with sera from 449 domestic pigs, provided by the Secretary of Agriculture of the Federal District. Blood samples were collected between June and September 2014. A commercially available ELISA kit was used for the detection of IgG antibodies. High seroprevalence of antibodies to HEV was found, since 304 animals showed anti-HEV positive reactions (67.7%; 95% CI = 63.2%, 71.9%). The seropositivity presented no difference by gender or age. The results suggest that HEV circulates among domestic pigs in the Federal District and it can serve as a warning to the local public health system due to their possible involvement in human infections.</p></div