Populations of doubled haploids for genetic mapping in hexaploid winter triticale.

To create a framework for genetic dissection of hexaploid triticale, six populations of doubled haploid (DH) lines were developed from pairwise hybrids of high-yielding winter triticale cultivars. The six populations comprise between 97 and 231 genotyped DH lines each, totaling 957 DH lines. A consensus genetic map spans 4593.9 cM is composed of 1576 unique DArT markers. The maps reveal several structural rearrangements in triticale genomes. In preliminary tests of the populations and maps, markers specific to wheat segments of the engineered rye chromosome 1R (RM1B) were identified. Example QTL mapping of days to heading in cv. Krakowiak revealed loci on chromosomes 2BL and 2R responsible for extended vernalization requirement, and candidate genes were identified. The material is available to all parties interested in triticale genetics

Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x Triticosecale Wittmack)

BACKGROUND: While the genetic transformation of the major cereal crops has become relatively routine, to date only a few reports were published on transgenic triticale, and robust data on T-DNA integration and segregation have not been available in this species. RESULTS: Here, we present a comprehensive analysis of stable transgenic winter triticale cv. Bogo carrying the selectable marker gene HYGROMYCIN PHOSPHOTRANSFERASE (HPT) and a synthetic green fluorescent protein gene (gfp). Progeny of four independent transgenic plants were comprehensively investigated with regard to the number of integrated T-DNA copies, the number of plant genomic integration loci, the integrity and functionality of individual T-DNA copies, as well as the segregation of transgenes in T(1) and T(2) generations, which also enabled us to identify homozygous transgenic lines. The truncation of some integrated T-DNAs at their left end along with the occurrence of independent segregation of multiple T-DNAs unintendedly resulted in a single-copy segregant that is selectable marker-free and homozygous for the gfp gene. The heritable expression of gfp driven by the maize UBI-1 promoter was demonstrated by confocal laser scanning microscopy. CONCLUSIONS: The used transformation method is a valuable tool for the genetic engineering of triticale. Here we show that comprehensive molecular analyses are required for the correct interpretation of phenotypic data collected from the transgenic plants

Renormalization Group Flow Equations and the Phase Transition in O(N)-models

We derive and solve flow equations for a general O(N)-symmetric effective potential including wavefunction renormalization corrections combined with a heat-kernel regularization. We investigate the model at finite temperature and study the nature of the phase transition in detail. Beta functions, fixed points and critical exponents \beta, \nu, \delta and \eta for various N are independently calculated which allow for a verification of universal scaling relations.Comment: 34 pages, 3 tables, 11 postscript figures, LaTe

The expression of B7-H1 and B7-H4 molecules on immature myeloid and lymphoid dendritic cells in cord blood of healthy neonates.

The aim of our study was to estimate both B7-H1 and B7-H4 molecules on immature myeloid and lymphoid dendritic cells in umbilical cord blood of healthy neonates in comparison with peripheral blood of healthy adults. Thirty nine healthy full-term neonates from physiological single pregnancies and 27 healthy adults were included in the study. The expression of B7-H1 and B7-H4 was revealed using the immunofluorescence method. Statistical analysis was performed using a non-parametric test (Mann-Whitney U-Test). The percentages of BDCA-1+ dendritic cells with B7-H1 and B7-H4 expressions were significantly higher in peripheral blood of healthy adults (

On the Convergence of the Expansion of Renormalization Group Flow Equation

We compare and discuss the dependence of a polynomial truncation of the effective potential used to solve exact renormalization group flow equation for a model with fermionic interaction (linear sigma model) with a grid solution. The sensitivity of the results on the underlying cutoff function is discussed. We explore the validity of the expansion method for second and first-order phase transitions.Comment: 12 pages with 10 EPS figures included; revised versio

On the Connection Between Momentum Cutoff and Operator Cutoff Regularizations

Operator cutoff regularization based on the original Schwinger's proper-time formalism is examined. By constructing a regulating smearing function for the proper-time integration, we show how this regularization scheme simulates the usual momentum cutoff prescription yet preserves gauge symmetry even in the presence of the cutoff scales. Similarity between the operator cutoff regularization and the method of higher (covariant) derivatives is also observed. The invariant nature of the operator cutoff regularization makes it a promising tool for exploring the renormalization group flow of gauge theories in the spirit of Wilson-Kadanoff blocking transformation.Comment: 28 pages in plain TeX, no figures. revised and expande

Symmetry preserving regularization with a cutoff

A Lorentz and gauge symmetry preserving regularization method is proposed in 4 dimension based on momentum cutoff. We use the conditions of gauge invariance or freedom of shift of the loop-momentum to define the evaluation of the terms carrying Lorentz indices, e.g. proportional to k_{\mu}k_{\nu}. The remaining scalar integrals are calculated with a four dimensional momentum cutoff. The finite terms (independent of the cutoff) are unambiguous and agree with the result of dimensional regularization.Comment: 12 pages, 1 figure, v2 references adde

Perturbative and non-perturbative aspects of the proper time renormalization group

The renormalization group flow equation obtained by means of a proper time regulator is used to calculate the two loop beta function and anomalous dimension eta of the field for the O(N) symmetric scalar theory. The standard perturbative analysis of the flow equation does not yield the correct results for both beta and eta. We also show that it is still possible to extract the correct beta and eta from the flow equation in a particular limit of the infrared scale. A modification of the derivation of the Exact Renormalization Group flow, which involves a more general class of regulators, to recover the proper time renormalization group flow is analyzed.Comment: 26 pages.Latex.Version accepted for publicatio

Completeness and consistency of renormalisation group flows

We study different renormalisation group flows for scale dependent effective actions, including exact and proper-time renormalisation group flows. These flows have a simple one loop structure. They differ in their dependence on the full field-dependent propagator, which is linear for exact flows. We investigate the inherent approximations of flows with a non-linear dependence on the propagator. We check explicitly that standard perturbation theory is not reproduced. We explain the origin of the discrepancy by providing links to exact flows both in closed expressions and in given approximations. We show that proper-time flows are approximations to Callan-Symanzik flows. Within a background field formalism, we provide a generalised proper-time flow, which is exact. Implications of these findings are discussed.Comment: 33 pages, 15 figures, revtex, typos corrected, to be published in Phys.Rev.