Pioglitazone-Loaded PLGA Nanoparticles: Towards the Most Reliable Synthesis Method

Recent findings have proved the benefits of Pioglitazone (PGZ) against atherosclerosis and type 2 diabetes. Since the systematic and controllable release of this drug is of significant importance, encapsulation of this drug in nanoparticles (NPs) can minimize uncontrolled issues. In this context, drug delivery approaches based on several poly(lactic-co-glycolic acid) (PLGA) nanoparticles have been rising in popularity due to their promising capabilities. However, a fully reliable and reproducible synthetic methodology is still lacking. In this work, we present a rational optimization of the most critical formulation parameters for the production of PGZ-loaded PLGA NPs by the single emulsification-solvent evaporation or nanoprecipitation methods. We examined the influence of several variables (e.g., component concentrations, phases ratio, injection flux rate) on the synthesis of the PGZ-NPs. In addition, a comparison of these synthetic methodologies in terms of nanoparticle size, polydispersity index (PDI), zeta potential (ζp), drug loading (DL%), entrapment efficiency (EE%), and stability is offered. According to the higher entrapment efficiency content, enhanced storage time and suitable particle size, the nanoprecipitation approach appears to be the simplest, most rapid and most reliable synthetic pathway for these drug nanocarriers, and we demonstrated a very slow drug release in PBS for the best formulation obtained by this synthesis.Recent findings have proved the benefits of Pioglitazone (PGZ) against atherosclerosis and type 2 diabetes. Since the systematic and controllable release of this drug is of significant importance, encapsulation of this drug in nanoparticles (NPs) can minimize uncontrolled issues. In this context, drug delivery approaches based on several poly(lactic-co-glycolic acid) (PLGA) nanoparticles have been rising in popularity due to their promising capabilities. However, a fully reliable and reproducible synthetic methodology is still lacking. In this work, we present a rational optimization of the most critical formulation parameters for the production of PGZ-loaded PLGA NPs by the single emulsification-solvent evaporation or nanoprecipitation methods. We examined the influence of several variables (e.g., component concentrations, phases ratio, injection flux rate) on the synthesis of the PGZ-NPs. In addition, a comparison of these synthetic methodologies in terms of nanoparticle size, polydispersity index (PDI), zeta potential (ζp), drug loading (DL%), entrapment efficiency (EE%), and stability is offered. According to the higher entrapment efficiency content, enhanced storage time and suitable particle size, the nanoprecipitation approach appears to be the simplest, most rapid and most reliable synthetic pathway for these drug nanocarriers, and we demonstrated a very slow drug release in PBS for the best formulation obtained by this synthesis

Metamorphosis of a Quantum Hall Bilayer State into a Composite Fermion Metal

Composite fermion metal states emerge in quantum Hall bilayers at total Landau level filling factor $\nu_T$=1 when the tunneling gap collapses by application of in-plane components of the external magnetic field. Evidence of this transformation is found in the continua of spin excitations observed by inelastic light scattering below the spin-wave mode at the Zeeman energy. The low-lying spin modes are interpreted as quasiparticle excitations with simultaneous changes in spin orientation and composite fermion Landau level index.Comment: 4 pages 4 figure

Observation of collapse of pseudospin order in bilayer quantum Hall ferromagnets

The Hartree-Fock paradigm of bilayer quantum Hall states with finite tunneling at filling factor $\nu$=1 has full pseudospin ferromagnetic order with all the electrons in the lowest symmetric Landau level. Inelastic light scattering measurements of low energy spin excitations reveal major departures from the paradigm at relatively large tunneling gaps. The results indicate the emergence of a novel correlated quantum Hall state at $\nu$=1 characterized by reduced pseudospin order. Marked anomalies occur in spin excitations when pseudospin polarization collapses by application of in-plane magnetic fields.Comment: ReVTeX4, 4 pages, 3 EPS figure

Soft Magnetorotons and Broken-Symmetry States in Bilayer Quantum Hall Ferromagnets

The recent report on the observation of soft magnetorotons in the dispersion of charge-density excitations across the tunneling gap in coupled bilayers at total Landau level filling factor $\nu_T=1$ is reviewed. The inelastic light scattering experiments take advantage of the breakdown of wave-vector conservation that occurs under resonant excitation. The results offer evidence that in the quantum Hall state there is a roton that softens and sharpens markedly when the phase boundary for transitions to highly-correlated compressible states is approached. These findings are interpreted with Hartree-Fock evaluations of the dynamic structure factor. The model includes the effect of disorder in the breakdown of wave-vector conservation and resonance enhancement profiles within a phenomenological approach. These results link the softening of magnetorotons to enhanced excitonic Coulomb interactions in the ferromagnetic bilayers.Comment: 6 pages, 5 figures; conference: EP2DS-1

Green fluorescent Protein-based pH indicators for in vivo use: a review

Green fluorescent protein (GFP) and its variants have been used as fluorescent reporters in a variety of applications for monitoring dynamic processes in cells and organisms, including gene expression, protein localization, and intracellular dynamics. GFP fluorescence is stable, species-independent, and can be monitored noninvasively in living cells by fluorescence microscopy, flow cytometry, or macroscopic imaging techniques. Owing to the presence of a phenol group on the chromophore, most GFP variants display pH-sensitive absorption and fluorescence bands. Such behavior has been exploited to genetically engineer encodable pH indicators for studies of pH regulation within specific intracellular compartments that cannot be probed using conventional pH-sensitive dyes. These pH indicators contributed to shedding light on a number of cell functions for which intracellular pH is an important modulator. In this review we discuss the photophysical properties that make GFPs so special as pH indicators for in vivo use and we describe the probes that are utilized most by the scientific community.Green fluorescent protein (GFP) and its variants have been used as fluorescent reporters in a variety of applications for monitoring dynamic processes in cells and organisms, including gene expression, protein localization, and intracellular dynamics. GFP fluorescence is stable, species-independent, and can be monitored noninvasively in living cells by fluorescence microscopy, flow cytometry, or macroscopic imaging techniques. Owing to the presence of a phenol group on the chromophore, most GFP variants display pH-sensitive absorption and fluorescence bands. Such behavior has been exploited to genetically engineer encodable pH indicators for studies of pH regulation within specific intracellular compartments that cannot be probed using conventional pH-sensitive dyes. These pH indicators contributed to shedding light on a number of cell functions for which intracellular pH is an important modulator. In this review we discuss the photophysical properties that make GFPs so special as pH indicators for in vivo use and we describe the probes that are utilized most by the scientific community. © 2008 Springer-Verlag

Intersubband absorption linewidth in GaAs quantum wells due to scattering by interface roughness, phonons, alloy disorder, and impurities

We calculate the intersubband absorption linewidth in quantum wells (QWs) due to scattering by interface roughness, LO phonons, LA phonons, alloy disorder, and ionized impurities, and compare it with the transport energy broadening that corresponds to the transport relaxation time related to electron mobility. Numerical calculations for GaAs QWs clarify the different contributions of each individual scattering mechanism to absorption linewidth and transport broadening. Interface roughness scattering contributes about an order of magnitude more to linewidth than to transport broadening, because the contribution from the intrasubband scattering in the first excited subband is much larger than that in the ground subband. On the other hand, LO phonon scattering (at room temperature) and ionized impurity scattering contribute much less to linewidth than to transport broadening. LA phonon scattering makes comparable contributions to linewidth and transport broadening, and so does alloy disorder scattering. The combination of these contributions with significantly different characteristics makes the absolute values of linewidth and transport broadening very different, and leads to the apparent lack of correlation between them when a parameter, such as temperature or alloy composition, is changed. Our numerical calculations can quantitatively explain the previously reported experimental results.Comment: 17 pages, including 15 figure

Observation of soft magnetorotons in bilayer quantum Hall ferromagnets

Inelastic light scattering measurements of low-lying collective excitations of electron double layers in the quantum Hall state at total filling nu_T=1 reveal a deep magnetoroton in the dispersion of charge-density excitations across the tunneling gap. The roton softens and sharpens markedly when the phase boundary for transitions to highly correlated compressible states is approached. The findings are interpreted with Hartree-Fock evaluations that link soft magnetorotons to enhanced excitonic Coulomb interactions and to quantum phase transitions in the ferromagnetic bilayers.Comment: ReVTeX4, 4 pages, 4 EPS figure

Spectroscopy of soft modes and quantum phase transitions in coupled electron bilayers

Strongly-correlated two-dimensional electrons in coupled semiconductor bilayers display remarkable broken symmetry many-body states under accessible and controllable experimental conditions. In the cases of continuous quantum phase transitions soft collective modes drive the transformations that link distinct ground states of the electron double layers. In this paper we consider results showing that resonant inelastic light scattering methods detect soft collective modes of the double layers and probe their evolution with temperature and magnetic field. The light scattering experiments offer venues of research of fundamental interactions and continuous quantum phase transitions in low-dimensional electron liquids.Comment: 10 pages, 7 figure

Graphene promotes axon elongation through local stall of Nerve Growth Factor signaling endosomes

Several works reported increased differentiation of neuronal cells grown on graphene; however, the molecular mechanism driving axon elongation on this material has remained elusive. Here, we study the axonal transport of nerve growth factor (NGF), the neurotrophin supporting development of peripheral neurons, as a key player in the time course of axonal elongation of dorsal root ganglion neurons on graphene. We find that graphene drastically reduces the number of retrogradely transported NGF vesicles in favor of a stalled population in the first two days of culture, in which the boost of axon elongation is observed. This correlates with a mutual charge redistribution, observed via Raman spectroscopy and electrophysiological recordings. Furthermore, ultrastructural analysis indicates a reduced microtubule distance and an elongated axonal topology. Thus, both electrophysiological and structural effects can account for graphene action on neuron development. Unraveling the molecular players underneath this interplay may open new avenues for axon regeneration applications